The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IF: Use at an assay dependent dilution. Recommended for use with avidin and streptavidin amplification systems for fluorescence microscopy. Acetone fixation of the antigen source is recommended prior to staining. Spot suspension of bacteria onto slide. Air dry. Fix slide with 100% acetone for 5 minutes at room temperature. Air dry. Stain.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Shigella is a genus of Gram-negative, non-motile, non-spore forming rod-shaped bacteria closely related to Escherichia coli and Salmonella. The causative agent of human shigellosis, Shigella also cause disease in other primates, but not in other mammals.
Shigella species are classified by four serogroups:
* Serogroup A: S. dysenteriae (12 serotypes)
* Serogroup B: S. flexneri (6 serotypes)
* Serogroup C: S. boydii (23 serotypes)
* Serogroup D: S. sonnei (1 serotype)
Shigella infection causes dysentery that results in the destruction of the epithelial cells of the intestinal mucosa in the cecum and rectum. Some strains produce enterotoxin and Shiga toxin, similar to the verotoxin of E. coli O157:H7.