Overview

  • Product name
    Anti-Shigella antibody (HRP)
    See all Shigella primary antibodies
  • Description
    Rabbit polyclonal to Shigella (HRP)
  • Host species
    Rabbit
  • Conjugation
    HRP
  • Specificity
    Rabbit polyclonal to Shigella (HRP)(ab19952) is polyvalent for Shigella antigens. It reacts with Shigella species including sonnei. Antiserum is not absorbed.
  • Tested applications
    Suitable for: ICC/IFmore details
  • Species reactivity
    Reacts with related Enterobacteriaceae. Not yet tested in other species.
  • Immunogen

    Mixture of S. boydii (ATCC #8700), S. flexneri (ATCC #29903) and S. dysenteriae (ATCC #13313)

  • General notes

    Rabbit polyclonal to Shigella (HRP) consists of the purified IgG fraction covalently coupled with highly purified preparation of horseradish peroxidase (RZ>3). Care is taken to ensure adequate conjugation while preserving maximum enzyme activity. Estimated molar HRP: IgG substitution is 2-3.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C.
  • Storage buffer
    pH: 7.20
    Preservative: 0.002% Thimerosal (merthiolate)
    Constituents: 0.0268% PBS, 1% BSA
  • Concentration information loading...
  • Purity
    Protein A purified
  • Purification notes
    Free horseradish peroxidase enzyme is not present.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab19952 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF
  • Application notes
    IF: Use at an assay dependent dilution.
    Acetone fixation of the antigen source is recommended prior to staining. Spot suspension of bacteria onto slide. Air dry. Fix slide with 100% Acetone for 5 minutes at room temperature. Air dry. Stain.
    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Relevance
      Shigella is a genus of Gram-negative, non-motile, non-spore forming rod-shaped bacteria closely related to Escherichia coli and Salmonella. The causative agent of human shigellosis, Shigella also cause disease in other primates, but not in other mammals. Shigella species are classified by four serogroups: * Serogroup A: S. dysenteriae (12 serotypes) * Serogroup B: S. flexneri (6 serotypes) * Serogroup C: S. boydii (23 serotypes) * Serogroup D: S. sonnei (1 serotype) Shigella infection causes dysentery that results in the destruction of the epithelial cells of the intestinal mucosa in the cecum and rectum. Some strains produce enterotoxin and Shiga toxin, similar to the verotoxin of E. coli O157:H7.
    • Alternative names
      • S. boydii antibody
      • S. dysenteriae antibody
      • S. flexneri antibody
      • S. sonnei antibody
      • Shigella Boydii antibody
      • Shigella Dysenteriae antibody
      • Shigella Flexneri antibody
      • Shigella Sonnei antibody
      see all

    References

    ab19952 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    1-2 of 2 Abreviews or Q&A

    Answer

    Thank you for contacting us.

    I am sorry to hear that you have been experiencing issues when using this product in ELISA. As stated on the datasheet of this product; Shigella is a genus of Gram-negative, non-motile, non-spore forming rod-shaped bacteria closely related to Escherichia coli and Salmonella. The product has not been cross absorbed to reduce E.coli reactivity and as such it is very likely that reactivity against E.coli will occur.

    This product is covered by our Abpromise guarantee for detection of Shigella in ELISA. We are happy provide scientific support at any time. If you are using the product in species and applications listed on the datasheet and contact us within six months of purchase, we are also happy to replace or refund the product should we not be able to help you to resolve the issue. More information on our Abpromise may be found at the following link:

    https://www.abcam.com/index.html?pageconfig=abpromise

    I hope that this information is helpful. Please let me know if you have any questions or there are other ways that Abcam may help you meet your research goals.

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    Question
    Answer

    Thank you for contacting Abcam.

    Please find attached the Certificate of Compliances for the 6 antibodies that you purchased recently.

    If there is anything else I can help you with, please let me know.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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