Product nameAnti-Shigella antibody (HRP)
See all Shigella primary antibodies
DescriptionRabbit polyclonal to Shigella (HRP)
SpecificityRabbit polyclonal to Shigella (HRP)(ab19952) is polyvalent for Shigella antigens. It reacts with Shigella species including sonnei. Antiserum is not absorbed.
Tested applicationsSuitable for: ICC/IFmore details
Species reactivityReacts with related Enterobacteriaceae. Not yet tested in other species.
Mixture of S. boydii (ATCC #8700), S. flexneri (ATCC #29903) and S. dysenteriae (ATCC #13313)
Rabbit polyclonal to Shigella (HRP) consists of the purified IgG fraction covalently coupled with highly purified preparation of horseradish peroxidase (RZ>3). Care is taken to ensure adequate conjugation while preserving maximum enzyme activity. Estimated molar HRP: IgG substitution is 2-3.
Storage instructionsShipped at 4°C. Store at +4°C.
Storage bufferpH: 7.20
Preservative: 0.002% Thimerosal (merthiolate)
Constituents: 0.0268% PBS, 1% BSA
Concentration information loading...
PurityProtein A purified
Purification notesFree horseradish peroxidase enzyme is not present.
- TMB ELISA Substrate (Highest Sensitivity) (ab171522)
- TMB ELISA Substrate (High Sensitivity) (ab171523)
- TMB ELISA Substrate (Fast Kinetic Rate) (ab171524)
- TMB ELISA Substrate (Slow Kinetic Rate) (ab171525)
- TMB ELISA Substrate (Slower Kinetic Rate) (ab171526)
- TMB ELISA Substrate (Slowest Kinetic Rate) (ab171527)
- 450 nm Stop Solution for TMB Substrate (ab171529)
- 650 nm Stop Solution for TMB Substrate (ab171531)
- Immunoassay Blocking Buffer (ab171534)
- Immunoassay Blocking (BSA Free) (ab171535)
Our Abpromise guarantee covers the use of ab19952 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Acetone fixation of the antigen source is recommended prior to staining. Spot suspension of bacteria onto slide. Air dry. Fix slide with 100% Acetone for 5 minutes at room temperature. Air dry. Stain.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
RelevanceShigella is a genus of Gram-negative, non-motile, non-spore forming rod-shaped bacteria closely related to Escherichia coli and Salmonella. The causative agent of human shigellosis, Shigella also cause disease in other primates, but not in other mammals. Shigella species are classified by four serogroups: * Serogroup A: S. dysenteriae (12 serotypes) * Serogroup B: S. flexneri (6 serotypes) * Serogroup C: S. boydii (23 serotypes) * Serogroup D: S. sonnei (1 serotype) Shigella infection causes dysentery that results in the destruction of the epithelial cells of the intestinal mucosa in the cecum and rectum. Some strains produce enterotoxin and Shiga toxin, similar to the verotoxin of E. coli O157:H7.
- S. boydii antibody
- S. dysenteriae antibody
- S. flexneri antibody
ab19952 has not yet been referenced specifically in any publications.