Recombinant
RabMAb

Recombinant Anti-SHIP-1 antibody [EP378Y] - BSA and Azide free (ab190551)

Overview

  • Product name

    Anti-SHIP-1 antibody [EP378Y] - BSA and Azide free
    See all SHIP-1 primary antibodies
  • Description

    Rabbit monoclonal [EP378Y] to SHIP-1 - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, Flow Cyt, IHC-P, ICC/IF, IPmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide within Human SHIP-1 aa 1100 to the C-terminus (C terminal). The exact sequence is proprietary.

  • Positive control

    • Human spleen tissue, Daudi cell lysate.
  • General notes

    ab190551 is a PBS-only buffer format of ab45142. Please refer to ab45142 for recommended dilutions, protocols, and image data.

    Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab190551 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
Flow Cyt
IHC-P
ICC/IF
IP
  • Application notes
    Flow Cyt: 1/20.
    IHC-P: 1/100 - 1/500. (Antigen retrieval is recommended).
    IP: 1/50.
    WB: 1/50000. Detects a band of approximately 140 kDa (predicted molecular weight: 133 kDa).

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function

      Phosphatidylinositol (PtdIns) phosphatase that specifically hydrolyzes the 5-phosphate of phosphatidylinositol-3,4,5-trisphosphate (PtdIns(3,4,5)P3) to produce PtdIns(3,4)P2, thereby negatively regulating the PI3K (phosphoinositide 3-kinase) pathways. Acts as a negative regulator of B-cell antigen receptor signaling. Mediates signaling from the FC-gamma-RIIB receptor (FCGR2B), playing a central role in terminating signal transduction from activating immune/hematopoietic cell receptor systems. Acts as a negative regulator of myeloid cell proliferation/survival and chemotaxis, mast cell degranulation, immune cells homeostasis, integrin alpha-IIb/beta-3 signaling in platelets and JNK signaling in B-cells. Regulates proliferation of osteoclast precursors, macrophage programming, phagocytosis and activation and is required for endotoxin tolerance. Involved in the control of cell-cell junctions, CD32a signaling in neutrophils and modulation of EGF-induced phospholipase C activity. Key regulator of neutrophil migration, by governing the formation of the leading edge and polarization required for chemotaxis. Modulates FCGR3/CD16-mediated cytotoxicity in NK cells. Mediates the activin/TGF-beta-induced apoptosis through its Smad-dependent expression. May also hydrolyze PtdIns(1,3,4,5)P4, and could thus affect the levels of the higher inositol polyphosphates like InsP6.
    • Tissue specificity

      Specifically expressed in immune and hematopoietic cells. Expressed in bone marrow and blood cells. Levels vary considerably within this compartment. Present in at least 74% of immature CD34+ cells, whereas within the more mature population of CD33+ cells, it is present in only 10% of cells. Present in the majority of T-cells, while it is present in a minority of B-cells (at protein level).
    • Sequence similarities

      Belongs to the inositol-1,4,5-trisphosphate 5-phosphatase family.
      Contains 1 SH2 domain.
    • Domain

      The SH2 domain interacts with tyrosine phosphorylated forms of proteins such as SHC1 or PTPN11/SHP-2. It competes with that of GRB2 for binding to phosphorylated SHC1 to inhibit the Ras pathway. It is also required for tyrosine phosphorylation.
      The NPXY sequence motif found in many tyrosine-phosphorylated proteins is required for the specific binding of the PID domain.
    • Post-translational
      modifications

      Tyrosine phosphorylated by the members of the SRC family after exposure to a diverse array of extracellular stimuli such as cytokines, growth factors, antibodies, chemokines, integrin ligands and hypertonic and oxidative stress. Phosphorylated upon IgG receptor FCGR2B-binding.
    • Cellular localization

      Cytoplasm. Membrane. Translocates to the plasma membrane when activated, translocation is probably due to different mechanisms depending on the stimulus and cell type. Partly translocated via its SH2 domain which mediates interaction with tyrosine phosphorylated receptors such as the FC-gamma-RIIB receptor (FCGR2B) or CD16/FCGR3. Tyrosine phosphorylation may also participate to membrane localization.
    • Information by UniProt
    • Database links

    • Alternative names

      • Inositol polyphosphate 5 phosphatase of 145kDa antibody
      • 4 antibody
      • 5-trisphosphate 5-phosphatase 1 antibody
      • hp51CN antibody
      • Inositol polyphosphate 5 phosphatase 145kDa antibody
      • Inositol polyphosphate 5 phosphatase antibody
      • Inositol polyphosphate-5-phosphatase of 145 kDa antibody
      • INPP5D antibody
      • MGC104855 antibody
      • MGC142140 antibody
      • MGC142142 antibody
      • p150Ship antibody
      • Phosphatidylinositol 3,4,5 trisphosphate 5 phosphatase 1 antibody
      • Phosphatidylinositol-3 antibody
      • SH2 containing inositol phosphatase isoform b antibody
      • SH2 domain containing inositol 5' phosphatase 1 antibody
      • SH2 domain containing inositol phosphatase 1 antibody
      • SH2 domain-containing inositol phosphatase 1 antibody
      • SH2 domain-containing inositol-5''-phosphatase 1 antibody
      • SHIP antibody
      • SHIP-1 antibody
      • SHIP1 antibody
      • SHIP1_HUMAN antibody
      • Signaling inositol polyphosphate 5 phosphatase SIP 145 antibody
      • SIP-145 antibody
      • SIP145 antibody
      see all

    References

    ab190551 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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