Key features and details
- Rabbit polyclonal to SHK
- Suitable for: IP
- Reacts with: Human
- Isotype: IgG
Product nameAnti-SHK antibody
See all SHK primary antibodies
DescriptionRabbit polyclonal to SHK
Tested applicationsSuitable for: IPmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse
Synthetic peptide within Human SHK aa 350-400. The exact sequence is proprietary.
Database link: Q9UHJ6
- IP: HEK-293T whole cell lysate.
Previously labelled as CARKL.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 6.8
Preservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab228422 was affinity purified using an epitope specific to SHK immobilized on solid support.
Our Abpromise guarantee covers the use of ab228422 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at an assay dependent concentration.
Use at 8 - 15 μl/mg lysate.
Tissue specificityStrongly expressed in liver, kidney and pancreas. Expressed at lower levels in placenta and heart. Very weakly expressed in lung and brain.
Sequence similaritiesBelongs to the FGGY kinase family.
- Information by UniProt
- Carbohydrate kinase like antibody
- Carbohydrate kinase like protein antibody
- Carbohydrate kinase-like protein antibody
SHK was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab228422 at 8 µl per reaction. Western blot was performed from the immunoprecipitate using ab228422 at 1/400 dilution.
Lane 1: ab228422 IP in HEK-293T whole cell lysate.
Lane 2: Control IgG IP in HEK-293T whole cell lysate.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab228422 has not yet been referenced specifically in any publications.