Key features and details
- Rabbit polyclonal to SHKBP1
- Suitable for: IP
- Reacts with: Human
- Isotype: IgG
Product nameAnti-SHKBP1 antibody
See all SHKBP1 primary antibodies
DescriptionRabbit polyclonal to SHKBP1
Tested applicationsSuitable for: IPmore details
Unsuitable for: WB
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Cow
Synthetic peptide within Human SHKBP1 aa 75-125. The exact sequence is proprietary.
Database link: Q8TBC3
- WB: SHKBP1 IP in Jurkat whole cell lysate.
ab241965 has not performed satisfactorily when used for WB of SHKBP1 in crude preparations (e.g. whole cell lysate). This antibody can be used for WB of enriched (e.g. immunoprecipitated) sources of SHKBP1.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8
Concentration information loading...
PurityImmunogen affinity purified
Purification notesAntibody was affinity purified using an epitope specific to SH3KBP1 Binding Protein 1/SHKBP1 immobilized on solid support.
Our Abpromise guarantee covers the use of ab241965 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at 2-10 µg/mg of lysate.|
FunctionInhibits CBL-SH3KBP1 complex mediated down-regulation of EGFR signaling by sequestration of SH3KBP1. Binds to SH3KBP1 and prevents its interaction with CBL and inhibits translocation of SH3KBP1 to EGFR containing vesicles upon EGF stimulation.
Sequence similaritiesBelongs to the KCTD3 family.
Contains 1 BTB (POZ) domain.
Contains 8 WD repeats.
- Information by UniProt
- PP203 antibody
- Sb1 antibody
- SH3KBP1 binding protein 1 antibody
SHKBP1 was immunoprecipitated from Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate (1 mg per IP reaction; 20% of IP loaded) prepared using NETN lysis buffer.
ab241965 used for IP at 6 µg per reaction. For WB 1 µg/ml.
Lane 1: ab241965 IP in Jurkat whole cell lysate.
Lane 2: Control IgG.
Chemiluminescence detection: 30 seconds.
ab241965 has not yet been referenced specifically in any publications.