Key features and details
- Rabbit polyclonal to SHMT2/SHMT
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Product nameAnti-SHMT2/SHMT antibody
See all SHMT2/SHMT primary antibodies
DescriptionRabbit polyclonal to SHMT2/SHMT
Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
Species reactivityReacts with: Mouse, Rat, Human
- WB: SK-MEL-30, NIH/3T3 and NBT-II cell lysates. IHC-P: Human lymph node, kidney, liver, colon, and skin tissue. ICC/IF: U-2 OS cells.
This product was previously labelled as SHMT2
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab224427 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 56 kDa.|
|IHC-P||1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|ICC/IF||Use a concentration of 0.25 - 2 µg/ml.
Fixation/Permeabilization: PFA/Triton X-100.
FunctionContributes to the de novo mitochondrial thymidylate biosynthesis pathway. Required to prevent uracil accumulation in mtDNA. Interconversion of serine and glycine. Associates with mitochondrial DNA.
PathwayOne-carbon metabolism; tetrahydrofolate interconversion.
Sequence similaritiesBelongs to the SHMT family.
Cellular localizationMitochondrion. Mitochondrion matrix > mitochondrion nucleoid. Mitochondrion inner membrane.
- Information by UniProt
- Epididymis secretory sperm binding protein Li 51e antibody
- GLY A+ antibody
- GLYA antibody
Paraffin-embedded human skin tissue stained for SHMT2/SHMT using ab224427 at 1/200 dilution in immunohistochemical analysis.
Paraffin-embedded human colon tissue stained for SHMT2/SHMT using ab224427 at 1/200 dilution in immunohistochemical analysis.
Paraffin-embedded human liver node tissue stained for SHMT2/SHMT using ab224427 at 1/200 dilution in immunohistochemical analysis.
Paraffin-embedded human kidney tissue stained for SHMT2/SHMT using ab224427 at 1/200 dilution in immunohistochemical analysis.
Anti-SHMT2/SHMT antibody (ab224427) at 1/100 dilution + SK-MEL-30 cell lysate
Predicted band size: 56 kDa
Paraffin-embedded human lymph node tissue stained for SHMT2/SHMT using ab224427 at 1/200 dilution in immunohistochemical analysis.
All lanes : Anti-SHMT2/SHMT antibody (ab224427) at 1/100 dilution
Lane 1 : NIH/3T3 (mouse embryo fibroblast cell line) cell lysate
Lane 2 : NBT-II cell lysate
Predicted band size: 56 kDa
PFA-fixed, Triton X-100 permeabilized U-2 OS (human bone osteosarcoma epithelial cell line) cells stained for SHMT2/SHMT (green) using ab224427 at 4 µg/ml in ICC/IF.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab224427 has not yet been referenced specifically in any publications.