Key features and details
- Rabbit polyclonal to SHMT2/SHMT
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human, Pig
- Isotype: IgG
Product nameAnti-SHMT2/SHMT antibody
See all SHMT2/SHMT primary antibodies
DescriptionRabbit polyclonal to SHMT2/SHMT
Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
Species reactivityReacts with: Human, Pig
Predicted to work with: Rabbit, Cow
Recombinant full length protein (His-T7-tag) corresponding to Human SHMT2/SHMT aa 30-504. Mature chain. Two N-terminal tags. Expressed in E.coli.
NAAQTQTGEANRGWTGQESLSDSDPEMWELLQREKDRQCRGLELIASENF CSRAALEALGSCLNNKYSEGYPGKRYYGGAEVVDEIELLCQRRALEAFDL DPAQWGVNVQPYSGSPANLAVYTALLQPHDRIMGLDLPDGGHLTHGYMSD VKRISATSIFFESMPYKLNPKTGLIDYNQLALTARLFRPRLIIAGTSAYA RLIDYARMREVCDEVKAHLLADMAHISGLVAAKVIPSPFKHADIVTTTTH KTLRGARSGLIFYRKGVKAVDPKTGREIPYTFEDRINFAVFPSLQGGPHN HAIAAVAVALKQACTPMFREYSLQVLKNARAMADALLERGYSLVSGGTDN HLVLVDLRPKGLDGARAERVLELVSITANKNTCPGDRSAITPGGLRLGAP ALTSRQFREDDFRRVVDFIDEGVNIGLEVKSKTAKLQDFKSFLLKDSETS QRLANLRQRVEQFARAFPMPGFDEH
Database link: P34897
- WB: Recombinant human SHMT2/SHMT protein; Human lung lysate; HEK-293T and HeLa cell lysates; Pig pancreas lysate. IHC-P: Human breast cancer, stomach, stomach cancer, rectum cancer, breast cancer, prostate and cerebrum tissue. ICC/IF: HeLa cells.
This product was previously labelled as SHMT2
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Purification notesAntigen-specific affinity chromatography followed by Protein A affinity chromatography.
Our Abpromise guarantee covers the use of ab233430 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.5 - 2 µg/ml. Predicted molecular weight: 56 kDa.|
|IHC-P||Use a concentration of 5 - 20 µg/ml.|
|ICC/IF||Use a concentration of 5 - 20 µg/ml.|
FunctionContributes to the de novo mitochondrial thymidylate biosynthesis pathway. Required to prevent uracil accumulation in mtDNA. Interconversion of serine and glycine. Associates with mitochondrial DNA.
PathwayOne-carbon metabolism; tetrahydrofolate interconversion.
Sequence similaritiesBelongs to the SHMT family.
Cellular localizationMitochondrion. Mitochondrion matrix > mitochondrion nucleoid. Mitochondrion inner membrane.
- Information by UniProt
- Epididymis secretory sperm binding protein Li 51e antibody
- GLY A+ antibody
- GLYA antibody
Anti-SHMT2/SHMT antibody (ab233430) at 1 µg/ml + Recombinant human SHMT2/SHMT protein
HRP-Linked Guinea pig anti-Rabbit at 1/2000 dilution
Predicted band size: 56 kDa
All lanes : Anti-SHMT2/SHMT antibody (ab233430) at 1 µg/ml
Lane 1 : Human lung lysate
Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate
Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate
Lane 4 : Pig pancreas lysate
All lanes : HRP-Linked Guinea pig anti-Rabbit at 1/2000 dilution
Predicted band size: 56 kDa
Formalin-fixed, paraffin-embedded human breast cancer tissue stained for SHNT2 using ab233430 at 20 μg/ml in immunohistochemical analysis. DAB staining.
Formalin-fixed, paraffin-embedded human stomach tissue stained for SHNT2 using ab233430 at 20 μg/ml in immunohistochemical analysis. DAB staining.
Formalin-fixed, paraffin-embedded human stomach cancer tissue stained for SHNT2 using ab233430 at 20 μg/ml in immunohistochemical analysis. DAB staining.
Formalin-fixed, paraffin-embedded human colorectal cancer tissue stained for SHNT2 using ab233430 at 20 μg/ml in immunohistochemical analysis. DAB staining.
Formalin-fixed, paraffin-embedded human prostate tissue stained for SHNT2 using ab233430 at 20 μg/ml in immunohistochemical analysis. DAB staining.
Formalin-fixed, paraffin-embedded human cerebrum tissue stained for SHNT2 using ab233430 at 20 μg/ml in immunohistochemical analysis. DAB staining.
HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for SHMT2/SHMT (green) using ab233430 at 20 μg/ml in ICC/IF. FITC staining.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab233430 has not yet been referenced specifically in any publications.