Anti-SHMT2/SHMT antibody (ab88664)
Key features and details
- Mouse polyclonal to SHMT2/SHMT
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-SHMT2/SHMT antibody
See all SHMT2/SHMT primary antibodies -
Description
Mouse polyclonal to SHMT2/SHMT -
Host species
Mouse -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant full length protein within Human SHMT2/SHMT. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: NP_005403.2 -
Positive control
- Human spleen tissue lysate. Hela cell lysate. SHMT2/SHMT transfected 293T cell lysate. HeLa cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.40
Constituent: 100% PBS -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab88664 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (2) |
Use a concentration of 1 µg/ml. Predicted molecular weight: 56 kDa.
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ICC/IF |
Use a concentration of 10 µg/ml.
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Notes |
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WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 56 kDa. |
ICC/IF
Use a concentration of 10 µg/ml. |
Target
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Function
Contributes to the de novo mitochondrial thymidylate biosynthesis pathway. Required to prevent uracil accumulation in mtDNA. Interconversion of serine and glycine. Associates with mitochondrial DNA. -
Pathway
One-carbon metabolism; tetrahydrofolate interconversion. -
Sequence similarities
Belongs to the SHMT family. -
Cellular localization
Mitochondrion. Mitochondrion matrix > mitochondrion nucleoid. Mitochondrion inner membrane. - Information by UniProt
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Database links
- Entrez Gene: 6472 Human
- Omim: 138450 Human
- SwissProt: P34897 Human
- Unigene: 741179 Human
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Alternative names
- Epididymis secretory sperm binding protein Li 51e antibody
- GLY A+ antibody
- GLYA antibody
see all
Images
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Anti-SHMT2/SHMT antibody (ab88664) at 1 µg/ml + human spleen tissue lysate at 50 µg
Predicted band size: 56 kDa
Observed band size: 56 kDa -
Anti-SHMT2/SHMT antibody (ab88664) at 1 µg/ml + Hela cell lysate at 50 µg
Predicted band size: 56 kDa
Observed band size: 56 kDa
Additional bands at: 42 kDa. We are unsure as to the identity of these extra bands. -
All lanes : Anti-SHMT2/SHMT antibody (ab88664) at 1 µg/ml
Lane 1 : SHMT2/SHMT transfected 293T cell lysate
Lane 2 : Non transfected 293T cell lysate
Lysates/proteins at 25 µg per lane.
Predicted band size: 56 kDa
Observed band size: 50 kDa why is the actual band size different from the predicted? -
ab88664, at 10 µg/ml, staining SHMT2/SHMT in Hela cells by Immunofluorescence.
Protocols
Datasheets and documents
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Datasheet download
References (1)
ab88664 has been referenced in 1 publication.
- Zhang L et al. Prognostic and therapeutic value of mitochondrial serine hydroxyl-methyltransferase 2 as a breast cancer biomarker. Oncol Rep 36:2489-2500 (2016). PubMed: 27666119