Overview

  • Product name

  • Description

    Mouse polyclonal to SHMT2/SHMT
  • Host species

    Mouse
  • Tested applications

    Suitable for: WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Full length protein corresponding to Human SHMT2/SHMT.
    Database link: NP_005403.2

  • Positive control

    • Human spleen tissue lysate. Hela cell lysate. SHMT2/SHMT transfected 293T cell lysate. HeLa cells.
  • General notes

     This product was previously labelled as SHMT2

     

Properties

Applications

Our Abpromise guarantee covers the use of ab88664 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 56 kDa.
ICC/IF Use a concentration of 10 µg/ml.

Target

  • Function

    Contributes to the de novo mitochondrial thymidylate biosynthesis pathway. Required to prevent uracil accumulation in mtDNA. Interconversion of serine and glycine. Associates with mitochondrial DNA.
  • Pathway

    One-carbon metabolism; tetrahydrofolate interconversion.
  • Sequence similarities

    Belongs to the SHMT family.
  • Cellular localization

    Mitochondrion. Mitochondrion matrix > mitochondrion nucleoid. Mitochondrion inner membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • Epididymis secretory sperm binding protein Li 51e antibody
    • GLY A+ antibody
    • GLYA antibody
    • glycine auxotroph A, human complement for hamster antibody
    • Glycine hydroxymethyltransferase antibody
    • GLYM_HUMAN antibody
    • HEL S 51e antibody
    • mitochondrial antibody
    • Serine hydroxymethyltransferase 2 (mitochondrial) antibody
    • Serine hydroxymethyltransferase antibody
    • Serine hydroxymethyltransferase mitochondrial antibody
    • Serine methylase antibody
    • SHMT 2 antibody
    • SHMT antibody
    • SHMT2 antibody
    see all

Images

  • Anti-SHMT2/SHMT antibody (ab88664) at 1 µg/ml + human spleen tissue lysate at 50 µg

    Predicted band size: 56 kDa
    Observed band size: 56 kDa

  • Anti-SHMT2/SHMT antibody (ab88664) at 1 µg/ml + Hela cell lysate at 50 µg

    Predicted band size: 56 kDa
    Observed band size: 56 kDa
    Additional bands at: 42 kDa. We are unsure as to the identity of these extra bands.

  • All lanes : Anti-SHMT2/SHMT antibody (ab88664) at 1 µg/ml

    Lane 1 : SHMT2/SHMT transfected 293T cell lysate
    Lane 2 : Non transfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.

    Predicted band size: 56 kDa
    Observed band size: 50 kDa
    why is the actual band size different from the predicted?

  • ab88664, at 10 µg/ml, staining SHMT2/SHMT in Hela cells by Immunofluorescence.

References

This product has been referenced in:

  • Zhang L  et al. Prognostic and therapeutic value of mitochondrial serine hydroxyl-methyltransferase 2 as a breast cancer biomarker. Oncol Rep 36:2489-2500 (2016). Read more (PubMed: 27666119) »
See 1 Publication for this product

Customer reviews and Q&As

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Read More
Application
Western blot
Sample
Human Cell lysate - whole cell (Jurkat)
Gel Running Conditions
Reduced Denaturing
Loading amount
20 µg
Specification
Jurkat
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 25°C

Abcam user community

Verified customer

Submitted May 02 2012

Application
Western blot
Sample
Human Cell lysate - whole cell (HeLa cells)
Gel Running Conditions
Reduced Denaturing
Loading amount
30 µg
Specification
HeLa cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C

Dr. Alfredo Castello

Verified customer

Submitted Apr 30 2010

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