Anti-Shoc2 / Sur8 antibody (ab229805)
Key features and details
- Rabbit polyclonal to Shoc2 / Sur8
- Suitable for: WB, ICC/IF, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Shoc2 / Sur8 antibody
See all Shoc2 / Sur8 primary antibodies -
Description
Rabbit polyclonal to Shoc2 / Sur8 -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IF, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Chicken, Cow, Orangutan -
Immunogen
Recombinant fragment corresponding to Human Shoc2/ Sur8 aa 1-100.
Database link: Q9UQ13 -
Positive control
- WB: MCF7, Jurkat and HepG2 whole cell lysates. IHC-P: Human lung and brain tissues. ICC/IF: HeLa cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol (glycerin, glycerine), PBS -
Concentration information loading...
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Purity
Protein G purified -
Purification notes
Purity >95%. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab229805 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/5000. Detects a band of approximately 65 kDa (predicted molecular weight: 65 kDa).
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ICC/IF |
1/50 - 1/200.
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IHC-P |
1/20 - 1/200.
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Notes |
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WB
1/500 - 1/5000. Detects a band of approximately 65 kDa (predicted molecular weight: 65 kDa). |
ICC/IF
1/50 - 1/200. |
IHC-P
1/20 - 1/200. |
Target
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Function
Regulatory subunit of protein phosphatase 1 (PP1c) that acts as a M-Ras/MRAS effector and participates in MAPK pathway activation. Upon M-Ras/MRAS activation, targets PP1c to specifically dephosphorylate the 'Ser-259' inhibitory site of RAF1 kinase and stimulate RAF1 activity at specialized signaling complexes. -
Involvement in disease
Defects in SHOC2 are the cause of Noonan syndrome-like with loose anagen hair (NSLAH) [MIM:607721]. NSLAH children display macrocephaly, high forehead, hypertelorism, palpebral ptosis, low-set and posteriorly rotated ears, short and webbed neck and pectus anomalies. Affected subjects also have easily pluckable, sparse, thin and slow-growing hair. -
Sequence similarities
Belongs to the SHOC2 family.
Contains 20 LRR (leucine-rich) repeats. -
Cellular localization
Cytoplasm. Nucleus. Translocates from cytoplasm to nucleus upon growth factor stimulation. - Information by UniProt
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Database links
- Entrez Gene: 423894 Chicken
- Entrez Gene: 511417 Cow
- Entrez Gene: 8036 Human
- Entrez Gene: 56392 Mouse
- Entrez Gene: 100173707 Orangutan
- Entrez Gene: 309548 Rat
- Omim: 602775 Human
- SwissProt: Q1L8Y7 Chicken
see all -
Alternative names
- KIAA0862 antibody
- Leucine-rich repeat protein SHOC-2 antibody
- Protein soc-2 homolog antibody
see all
Images
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All lanes : Anti-Shoc2 / Sur8 antibody (ab229805) at 1/500 dilution
Lane 1 : MCF7 (human breast adenocarcinoma cell line) whole cell lysate
Lane 2 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Lane 3 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate
Secondary
All lanes : Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 65 kDa
Observed band size: 65 kDa -
Paraffin-embedded human lung tissue stained for Shoc2 / Sur8 with ab229805 at 1/100 dilution in immunohistochemical analysis.
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Paraffin-embedded human brain tissue stained for Shoc2 / Sur8 with ab229805 at 1/100 dilution in immunohistochemical analysis.
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HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for Shoc2 / Sur8 (green) using ab229805 at 1/100 dilution in ICC/IF, followed by Alexa Fluor 488® congugated Goat Anti-Rabbit IgG (H+L).
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab229805 has not yet been referenced specifically in any publications.