Key features and details
- Rabbit polyclonal to Shoc2 / Sur8
- Suitable for: WB, ICC/IF, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-Shoc2 / Sur8 antibody
See all Shoc2 / Sur8 primary antibodies
DescriptionRabbit polyclonal to Shoc2 / Sur8
Tested applicationsSuitable for: WB, ICC/IF, IHC-Pmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Chicken, Cow, Orangutan
- WB: MCF7, Jurkat and HepG2 whole cell lysates. IHC-P: Human lung and brain tissues. ICC/IF: HeLa cells.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Constituents: 50% Glycerol (glycerin, glycerine), PBS, 0.03% Proclin 300
Concentration information loading...
PurityProtein G purified
Purification notesPurity >95%.
Our Abpromise guarantee covers the use of ab229805 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/5000. Detects a band of approximately 65 kDa (predicted molecular weight: 65 kDa).|
|ICC/IF||1/50 - 1/200.|
|IHC-P||1/20 - 1/200.|
FunctionRegulatory subunit of protein phosphatase 1 (PP1c) that acts as a M-Ras/MRAS effector and participates in MAPK pathway activation. Upon M-Ras/MRAS activation, targets PP1c to specifically dephosphorylate the 'Ser-259' inhibitory site of RAF1 kinase and stimulate RAF1 activity at specialized signaling complexes.
Involvement in diseaseDefects in SHOC2 are the cause of Noonan syndrome-like with loose anagen hair (NSLAH) [MIM:607721]. NSLAH children display macrocephaly, high forehead, hypertelorism, palpebral ptosis, low-set and posteriorly rotated ears, short and webbed neck and pectus anomalies. Affected subjects also have easily pluckable, sparse, thin and slow-growing hair.
Sequence similaritiesBelongs to the SHOC2 family.
Contains 20 LRR (leucine-rich) repeats.
Cellular localizationCytoplasm. Nucleus. Translocates from cytoplasm to nucleus upon growth factor stimulation.
- Information by UniProt
- KIAA0862 antibody
- Leucine-rich repeat protein SHOC-2 antibody
- Protein soc-2 homolog antibody
All lanes : Anti-Shoc2 / Sur8 antibody (ab229805) at 1/500 dilution
Lane 1 : MCF7 (human breast adenocarcinoma cell line) whole cell lysate
Lane 2 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Lane 3 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate
All lanes : Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 65 kDa
Observed band size: 65 kDa
Paraffin-embedded human lung tissue stained for Shoc2 / Sur8 with ab229805 at 1/100 dilution in immunohistochemical analysis.
Paraffin-embedded human brain tissue stained for Shoc2 / Sur8 with ab229805 at 1/100 dilution in immunohistochemical analysis.
HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for Shoc2 / Sur8 (green) using ab229805 at 1/100 dilution in ICC/IF, followed by Alexa Fluor 488® congugated Goat Anti-Rabbit IgG (H+L).
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab229805 has not yet been referenced specifically in any publications.