Overview

  • Product name

    Anti-Shoc2 / Sur8 antibody
    See all Shoc2 / Sur8 primary antibodies
  • Description

    Rabbit polyclonal to Shoc2 / Sur8
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    A 21 amino acid peptide near the N terminus of Human Shoc2/ Sur8 (NP_031399).

  • Positive control

    • Jurkat cell lysate; Human spleen tissue

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    Preservative: 0.02% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab106430 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 65 kDa.
IHC-P Use a concentration of 5 µg/ml.

Target

  • Function

    Regulatory subunit of protein phosphatase 1 (PP1c) that acts as a M-Ras/MRAS effector and participates in MAPK pathway activation. Upon M-Ras/MRAS activation, targets PP1c to specifically dephosphorylate the 'Ser-259' inhibitory site of RAF1 kinase and stimulate RAF1 activity at specialized signaling complexes.
  • Involvement in disease

    Defects in SHOC2 are the cause of Noonan syndrome-like with loose anagen hair (NSLAH) [MIM:607721]. NSLAH children display macrocephaly, high forehead, hypertelorism, palpebral ptosis, low-set and posteriorly rotated ears, short and webbed neck and pectus anomalies. Affected subjects also have easily pluckable, sparse, thin and slow-growing hair.
  • Sequence similarities

    Belongs to the SHOC2 family.
    Contains 20 LRR (leucine-rich) repeats.
  • Cellular localization

    Cytoplasm. Nucleus. Translocates from cytoplasm to nucleus upon growth factor stimulation.
  • Information by UniProt
  • Database links

  • Alternative names

    • KIAA0862 antibody
    • Leucine-rich repeat protein SHOC-2 antibody
    • Protein soc-2 homolog antibody
    • Protein Sur-8 homolog antibody
    • Ras-binding protein Sur-8 antibody
    • SHOC2 antibody
    • SHOC2_HUMAN antibody
    see all

Images

  • Lane 1 : Anti-Shoc2 / Sur8 antibody (ab106430) at 1 µg/ml
    Lane 2 : Anti-Shoc2 / Sur8 antibody (ab106430) at 2 µg/ml

    All lanes : Jurkat cell lysate

    Lysates/proteins at 15 µg per lane.

    Predicted band size: 65 kDa

  • ab106430, at 5 µg/ml, staining Shoc2 / Sur8 in paraffin embedded Human spleen tissue by Immunohistochemistry.

References

This product has been referenced in:

  • Xie CM  et al. The FBXW7-SHOC2-Raptor Axis Controls the Cross-Talks between the RAS-ERK and mTORC1 Signaling Pathways. Cell Rep 26:3037-3050.e4 (2019). Read more (PubMed: 30865892) »
See 1 Publication for this product

Customer reviews and Q&As

Answer

Gracias por ponerte en contacto con nosotros y alertarnos del problema experimentado con nuestros productos.

Cualquier comentario o queja procedente de nuestros clientes es muy importante para mejorar y monitorizar la calidad de nuestros productos.

La información que me facilitas es muy útil para poder entender por qué el anticuerpo no ha funcionado, pero nos ayudaría mucho que nos ampliaras la información referente al protocolo seguido.

Te envío un cuestionario que contiene todas algunas preguntas, y si pudieras, además del cuestionario completo, enviarnos una imagen nos seria de gran utilidad para intentar resolver el problema.

Nuestra garantía Abpromise garantiza que los productos funcionan tal y como se especifica en su ficha técnica, y en caso contrario, estaremos encantados de ofrecer soporte científico que ayude a mejorar los resultados. Si aun así el producto sigue sin funcionar correctamente, y éste se adquirió en los últimos seis meses, estaremos encantados de reemplazar o reembolsar el valor del anticuerpo.

Gracias por tu comprensión, y quedo a la espera de tu respuesta.

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