Overview

  • Product name
  • Description
    Mouse monoclonal to SHOX2
  • Host species
    Mouse
  • Tested applications
    Suitable for: WB, IHC-Frmore details
  • Species reactivity
    Reacts with: Rat, Human
  • Immunogen

    Recombinant fragment: SPELKDRKDD AKGMEDEGQT KIKQRRSRTN FTLEQLNELE RLFDETHYPD AFMREELSQR LGLSEARVQV WFQNRRAKCR KQENQLHK, corresponding to amino acids 117-205 of Human SHOX2

Properties

Applications

Our Abpromise guarantee covers the use of ab55740 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 35 kDa.
IHC-Fr 1/500.

Target

  • Function
    May be a growth regulator and have a role in specifying neural systems involved in processing somatosensory information, as well as in face and body structure formation.
  • Tissue specificity
    Expressed in heart, skeletal muscle, liver, lung, bone marrow fibroblast, pancreas and placenta.
  • Sequence similarities
    Belongs to the paired homeobox family. Bicoid subfamily.
    Contains 1 homeobox DNA-binding domain.
  • Developmental stage
    Expressed during cranofacial development as well as in heart.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Homeobox protein Og12X antibody
    • OG 12 antibody
    • OG 12X antibody
    • OG12 antibody
    • OG12X antibody
    • OGI 2X antibody
    • OGI2X antibody
    • Paired related homeobox protein SHOT antibody
    • Paired-related homeobox protein SHOT antibody
    • Short stature homeobox 2 antibody
    • Short stature homeobox homolog antibody
    • Short stature homeobox protein 2 antibody
    • SHOT antibody
    • SHOX 2 antibody
    • SHOX homologous gene on chromosome 3 antibody
    • SHOX2 antibody
    • SHOX2_HUMAN antibody
    see all

Images

  • ab55740 staining SHOX2 in mouse embryonic spinal cord tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with paraformaldehyde and blocked with 10% serum for 30 minutes at 20°C. Samples were incubated with primary antibody (1/500 in PBS + 10% horse serum + 0.1% Triton X-100 in PBS) for 16 hours at 4°C. An Alexa Fluor® 488-conjugated donkey anti-mouse IgG polyclonal (1/1000) was used as the secondary antibody.

    See Abreview

  • SHOX2 antibody (ab55740) at 1ug/lane + PC-12 cell lysate at 25ug/lane.

References

This product has been referenced in:
See 1 Publication for this product

Customer reviews and Q&As

Application
Immunohistochemistry (Frozen sections)
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 20°C
Sample
Mouse Tissue sections (Mouse embryonic spinal cord (cryosections))
Specification
Mouse embryonic spinal cord (cryosections)
Permeabilization
No
Fixative
Paraformaldehyde

Dr. Frederic Clotman

Verified customer

Submitted Jan 27 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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