Western blot abreview for Anti-SHOX2 antibody

Average
Abreviews
Application
Western blot
Sample
Mouse Tissue lysate - whole (Heart)
Gel Running Conditions
Reduced Denaturing (12%)
Loading amount
250 µg
Specification
Heart
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Other product details

Incubation time
18 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: PBS + 0.05% Tween-20 + 5% NFDM
Concentration
1 µg/ml

Secondary antibody

Dilution
1/5000
Name
Non-Abcam antibody was used: 2¯ Goat anti-Rabbit HRP Ab
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase

Detection

Detection method
ECL
Negative control
Apex of P28 mouse heart.
Exposure
10 second(s)
Bands
Specific: 35 kDa

Additional data

Additional Notes
Antibody did not work for IF - mouse heart cryosection.

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Submitted May 09 2017

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