Western blot abreview for Anti-SHOX2 antibody

Good
Abreviews
Application
Western blot
Sample
Mouse Cell lysate - whole cell (ATDC5 cell lines-chondrocyte)
Gel Running Conditions
Reduced Denaturing (4-12% Bis-Tris)
Loading amount
25 µg
Specification
ATDC5 cell lines-chondrocyte
Blocking step
Odyssey Blocking Buffer (PBS) as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 50% · Temperature: 25°C

Other product details

Incubation time
20 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 1/2 Odyssey Blocking Buffer
Concentration
1 µg/ml

Secondary antibody

Dilution
1/10000
Name
Non-Abcam antibody was used: Donkey Anti-Rabbit IgG Polyclonal Antibody (IRDye®
Host species: Donkey
Clonality: Polyclonal
Conjugation: IRDye® 800CW

Detection

Detection method
Licor-Odyssey Imaging System
Exposure
5 second(s)
Bands
Specific: 45 kDa Non-specific: 14 kDa
Positive control
SHOX2 overexpression by Lenti-virus.

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Submitted Apr 24 2019

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