Anti-SHP1 antibody (ab227503)

Rabbit polyclonal SHP1 antibody. Validated in WB, IP, IHC and tested in Mouse, Rat, Human. Immunogen corresponding to recombinant fragment.

Overview

  • Product name

  • Description

    Rabbit polyclonal to SHP1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IP, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
    Predicted to work with: Cow
  • Immunogen

    Recombinant fragment within Human SHP1 (internal sequence). The exact sequence is proprietary.
    Database link: P29350

  • Positive control

    • WB: Rat liver extract; HeLa S3, HepG2 and NIH/3T3 whole cell lysates. IHC-P: OVCA xenograft tissue. IP: HeLa whole cell lysate (ab150035).

Properties

Applications

Our Abpromise guarantee covers the use of ab227503 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 68 kDa.
IP 1/100 - 1/500.
IHC-P 1/100 - 1/1000.

Target

  • Function

    Plays a key role in hematopoiesis. This PTPase activity may directly link growth factor receptors and other signaling proteins through protein-tyrosine phosphorylation. The SH2 regions may interact with other cellular components to modulate its own phosphatase activity against interacting substrates. Together with MTUS1, induces UBE2V2 expression upon angiotensin II stimulation.
  • Tissue specificity

    Isoform 1 is expressed in hematopoietic cells. Isoform 2 is expressed in non-hematopoietic cells.
  • Sequence similarities

    Belongs to the protein-tyrosine phosphatase family. Non-receptor class 2 subfamily.
    Contains 2 SH2 domains.
    Contains 1 tyrosine-protein phosphatase domain.
  • Post-translational
    modifications

    Phosphorylated on serine and tyrosine residues.
  • Cellular localization

    Cytoplasm. Nucleus. In neurons, translocates into the nucleus after treatment with angiotensin II.
  • Information by UniProt
  • Database links

  • Alternative names

    • 70Z-SHP antibody
    • EC 3.1.3.48 antibody
    • HCP antibody
    • HCPH antibody
    • Hematopoietic cell phosphatase antibody
    • Hematopoietic cell protein tyrosine phosphatase antibody
    • Hematopoietic cell protein-tyrosine phosphatase antibody
    • HPTP1C antibody
    • Protein tyrosine phosphatase 1C antibody
    • Protein tyrosine phosphatase non receptor type 6 antibody
    • Protein tyrosine phosphatase SHP1 antibody
    • Protein-tyrosine phosphatase 1C antibody
    • protein-tyrosine phosphatase SHP 1 antibody
    • Protein-tyrosine phosphatase SHP-1 antibody
    • PTN6_HUMAN antibody
    • PTP 1C antibody
    • PTP-1C antibody
    • PTP1C antibody
    • Ptpn6 antibody
    • SH PTP 1 antibody
    • SH PTP1 antibody
    • SH-PTP1 antibody
    • SHP 1 antibody
    • SHP 1L antibody
    • SHP1 antibody
    • SHP1L antibody
    • tyrosine protein phosphatase non receptor type 6 antibody
    • Tyrosine-protein phosphatase non-receptor type 6 antibody
    see all

Images

  • Anti-SHP1 antibody (ab227503) at 1/1000 dilution + Rat liver extract at 50 µg

    Secondary
    HRP-conjugated anti-rabbit IgG

    Predicted band size: 68 kDa



    7.5% SDS-PAGE gel.

  • Paraffin-embedded OVCA xenograft tissue stained for SHP1 using ab227503 at 1/100 dilution in immunohistochemical analysis.

  • All lanes : Anti-SHP1 antibody (ab227503) at 1/500 dilution

    Lane 1 : HeLa S3 whole cell lysate
    Lane 2 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Secondary
    All lanes : HRP-conjugated anti-rabbit IgG

    Predicted band size: 68 kDa



    7.5% SDS-PAGE gel.

  • Anti-SHP1 antibody (ab227503) at 1/1000 dilution + NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate at 30 µg

    Secondary
    HRP-conjugated anti-rabbit IgG

    Predicted band size: 68 kDa



    7.5% SDS-PAGE gel.

  • SHP1 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate with 3 µg ab227503. Western blot was performed from the immunoprecipitate using ab227503 at 1/1000 dilution. Anti-Rabbit IgG was used as a secondary reagent.

    Lane 1: HeLa whole cell lysate.

    Lane 2: Control IP in HeLa whole cell lysate with 3 μg of pre-immune rabbit IgG .

    Lane 3: ab227503 IP in HeLa whole cell lysate.

References

ab227503 has not yet been referenced specifically in any publications.

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