Key features and details
- Goat polyclonal to SHP1
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Product nameAnti-SHP1 antibody
See all SHP1 primary antibodies
DescriptionGoat polyclonal to SHP1
SpecificityThis antibody is expected to recognise both reported isoforms (NP_536858.1and NP_002822.2). Please note this antibody was designed using the mouse sequence, which differs by 1 amino acid from the human sequence.
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Mouse, Rat, Human
- WB: Human Liver lysates, Mouse and Rat thymus tissue lysates; IHC-P: Human Thyroid tissue.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% Tris buffered saline, 0.5% BSA
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab60268 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||Use a concentration of 0.01 - 0.3 µg/ml. Detects a band of approximately 68 kDa (predicted molecular weight: 68 kDa).
A 1 hour primary incubation is recommended for this product.
FunctionPlays a key role in hematopoiesis. This PTPase activity may directly link growth factor receptors and other signaling proteins through protein-tyrosine phosphorylation. The SH2 regions may interact with other cellular components to modulate its own phosphatase activity against interacting substrates. Together with MTUS1, induces UBE2V2 expression upon angiotensin II stimulation.
Tissue specificityIsoform 1 is expressed in hematopoietic cells. Isoform 2 is expressed in non-hematopoietic cells.
Sequence similaritiesBelongs to the protein-tyrosine phosphatase family. Non-receptor class 2 subfamily.
Contains 2 SH2 domains.
Contains 1 tyrosine-protein phosphatase domain.
modificationsPhosphorylated on serine and tyrosine residues.
Cellular localizationCytoplasm. Nucleus. In neurons, translocates into the nucleus after treatment with angiotensin II.
- Information by UniProt
- 70Z-SHP antibody
- EC 126.96.36.199 antibody
- HCP antibody
Anti-SHP1 antibody (ab60268) at 0.1 µg/ml + Human Liver lysate (35µg protein in RIPA buffer)
Predicted band size: 68 kDa
Observed band size: 68 kDa
Primary incubation was 1 hour. Detected by chemiluminescence.
ab60268 (5 µg/ml) staining of paraffin embedded Human Thyroid. Steamed antigen retrieval with citrate buffer pH 6.
Lane 1 : Anti-SHP1 antibody (ab60268) at 0.01 µg/ml
Lane 2 : Anti-SHP1 antibody (ab60268) at 0.1 µg/ml
Lane 1 : Mouse Thymus lysate (35µg protein in RIPA buffer)
Lane 2 : Rat Thymus lysate (35µg protein in RIPA buffer)
Predicted band size: 68 kDa
ab60268 has been referenced in 1 publication.
- Liu HP et al. Association of supervillin with KIR2DL1 regulates the inhibitory signaling of natural killer cells. Cell Signal 23:487-96 (2011). WB, ICC/IF ; Human . PubMed: 21070852