Recombinant Anti-SHP1 antibody [EPR5519] (ab124942)
- Datasheet
- References (3)
- Protocols
Overview
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Product name
Anti-SHP1 antibody [EPR5519]
See all SHP1 primary antibodies -
Description
Rabbit monoclonal [EPR5519] to SHP1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, Flow Cyt, ICC/IFmore details -
Species reactivity
Reacts with: Human
Does not react with: Mouse, Rat -
Immunogen
Synthetic peptide within Human SHP1 aa 1-100. The exact sequence is proprietary.
Database link: P29350 -
Positive control
- Human tonsil tissue; Jurkat, Raji and lymph node lysates
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General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Dissociation constant (KD)
KD = 4.95 x 10 -11 M Learn more about KD -
Storage buffer
pH: 7.40
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR5519 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab124942 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000 - 1/10000. Predicted molecular weight: 68 kDa. | |
| IHC-P | 1/1000 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. | |
| Flow Cyt | 1/10 - 1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
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| ICC/IF | 1/250 - 1/500. |
Target
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Function
Plays a key role in hematopoiesis. This PTPase activity may directly link growth factor receptors and other signaling proteins through protein-tyrosine phosphorylation. The SH2 regions may interact with other cellular components to modulate its own phosphatase activity against interacting substrates. Together with MTUS1, induces UBE2V2 expression upon angiotensin II stimulation. -
Tissue specificity
Isoform 1 is expressed in hematopoietic cells. Isoform 2 is expressed in non-hematopoietic cells. -
Sequence similarities
Belongs to the protein-tyrosine phosphatase family. Non-receptor class 2 subfamily.
Contains 2 SH2 domains.
Contains 1 tyrosine-protein phosphatase domain. -
Post-translational
modificationsPhosphorylated on serine and tyrosine residues. -
Cellular localization
Cytoplasm. Nucleus. In neurons, translocates into the nucleus after treatment with angiotensin II. - Information by UniProt
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Database links
- Entrez Gene: 5777 Human
- Omim: 176883 Human
- SwissProt: P29350 Human
- Unigene: 63489 Human
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Alternative names
- 70Z-SHP antibody
- EC 3.1.3.48 antibody
- HCP antibody
see all
Images
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![Western blot - Anti-SHP1 antibody [EPR5519] (ab124942)](https://www.abcam.com/ps/products/124/ab124942/Images/ab124942-138985-anti-shp1-antibody-epr5519-western-blot.jpg)
Western blot - Anti-SHP1 antibody [EPR5519] (ab124942)All lanes : Anti-SHP1 antibody [EPR5519] (ab124942) at 1/1000 dilution
Lane 1 : Jurkat cell lysate
Lane 2 : Raji cell lysate
Lane 3 : lymph node lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : standard HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 68 kDa -
![Immunocytochemistry/ Immunofluorescence - Anti-SHP1 antibody [EPR5519] (ab124942)](https://www.abcam.com/ps/products/124/ab124942/Images/ab124942-261740-anti-shp1-antibody-epr5519-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-SHP1 antibody [EPR5519] (ab124942)Immunocytochemistry/Immunofluorescence analysis of Raji (human Burkitt's lymphoma) cells labelling SHP1 with purified ab124942 at a dilution of 1/500. Cells were fixed with 4% Paraformaldehyde and permeabilised with 0.1% tritonX-100. An Alexa Fluor® 488-conjugated goat anti-rabbit IgG (ab150077) at dilution of 1/1000 was used as the secondary antibody. Nuclei counterstained with DAPI (blue).
Secondary Only Control: PBS was used instead of the primary antibody as the negative control.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SHP1 antibody [EPR5519] (ab124942)](https://www.abcam.com/ps/products/124/ab124942/Images/ab124942-138984-anti-shp1-antibody-epr5519-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SHP1 antibody [EPR5519] (ab124942)ab124942, at a 1/1000 dilution, staining SHP1 in paraffin-embedded Human tonsil tissue by immunohistochemistry -
![Other - Anti-SHP1 antibody [EPR5519] (ab124942)](https://www.abcam.com/ps/products/124/ab124942/Images/ab124942-138983-anti-shp1-antibody-epr5519.jpg)
Other - Anti-SHP1 antibody [EPR5519] (ab124942)
Protocols
References
This product has been referenced in:
- Koh JS et al. Inhibition of STAT3 in gastric cancer: role of pantoprazole as SHP-1 inducer. Cell Biosci 8:50 (2018). Read more (PubMed: 30202514) »
- Gladkikh AA et al. Comparison of the mRNA expression profile of B-cell receptor components in normal CD5-high B-lymphocytes and chronic lymphocytic leukemia: a key role of ZAP70. Cancer Med 6:2984-2997 (2017). Read more (PubMed: 29125235) »
