Overview

  • Product name
    Anti-SHP1 antibody [EPR5519]
    See all SHP1 primary antibodies
  • Description
    Rabbit monoclonal [EPR5519] to SHP1
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-P, Flow Cyt, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Does not react with: Mouse, Rat
  • Immunogen

    Synthetic peptide within Human SHP1 aa 1-100. The exact sequence is proprietary.

  • Positive control
    • Human tonsil tissue; Jurkat, Raji and lymph node lysates
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab124942 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 68 kDa.
IHC-P 1/1000 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 
ICC/IF 1/250 - 1/500.

Target

  • Function
    Plays a key role in hematopoiesis. This PTPase activity may directly link growth factor receptors and other signaling proteins through protein-tyrosine phosphorylation. The SH2 regions may interact with other cellular components to modulate its own phosphatase activity against interacting substrates. Together with MTUS1, induces UBE2V2 expression upon angiotensin II stimulation.
  • Tissue specificity
    Isoform 1 is expressed in hematopoietic cells. Isoform 2 is expressed in non-hematopoietic cells.
  • Sequence similarities
    Belongs to the protein-tyrosine phosphatase family. Non-receptor class 2 subfamily.
    Contains 2 SH2 domains.
    Contains 1 tyrosine-protein phosphatase domain.
  • Post-translational
    modifications
    Phosphorylated on serine and tyrosine residues.
  • Cellular localization
    Cytoplasm. Nucleus. In neurons, translocates into the nucleus after treatment with angiotensin II.
  • Information by UniProt
  • Database links
    • Alternative names
      • 70Z-SHP antibody
      • EC 3.1.3.48 antibody
      • HCP antibody
      • HCPH antibody
      • Hematopoietic cell phosphatase antibody
      • Hematopoietic cell protein tyrosine phosphatase antibody
      • Hematopoietic cell protein-tyrosine phosphatase antibody
      • HPTP1C antibody
      • Protein tyrosine phosphatase 1C antibody
      • Protein tyrosine phosphatase non receptor type 6 antibody
      • Protein tyrosine phosphatase SHP1 antibody
      • Protein-tyrosine phosphatase 1C antibody
      • protein-tyrosine phosphatase SHP 1 antibody
      • Protein-tyrosine phosphatase SHP-1 antibody
      • PTN6_HUMAN antibody
      • PTP 1C antibody
      • PTP-1C antibody
      • PTP1C antibody
      • Ptpn6 antibody
      • SH PTP 1 antibody
      • SH PTP1 antibody
      • SH-PTP1 antibody
      • SHP 1 antibody
      • SHP 1L antibody
      • SHP1 antibody
      • SHP1L antibody
      • tyrosine protein phosphatase non receptor type 6 antibody
      • Tyrosine-protein phosphatase non-receptor type 6 antibody
      see all

    Images

    • Western blot - Anti-SHP1 antibody [EPR5519] (ab124942)
      Western blot - Anti-SHP1 antibody [EPR5519] (ab124942)
      All lanes : Anti-SHP1 antibody [EPR5519] (ab124942) at 1/1000 dilution

      Lane 1 : Jurkat cell lysate
      Lane 2 : Raji cell lysate
      Lane 3 : lymph node lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : standard HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 68 kDa

    • Immunocytochemistry/ Immunofluorescence - Anti-SHP1 antibody [EPR5519] (ab124942)
      Immunocytochemistry/ Immunofluorescence - Anti-SHP1 antibody [EPR5519] (ab124942)

      Immunocytochemistry/Immunofluorescence analysis of Raji (human Burkitt's lymphoma) cells labelling SHP1 with purified ab124942 at a dilution of 1/500. Cells were fixed with 4% Paraformaldehyde and permeabilised with 0.1% tritonX-100. An Alexa Fluor® 488-conjugated goat anti-rabbit IgG (ab150077) at dilution of 1/1000 was used as the secondary antibody. Nuclei counterstained with DAPI (blue).

      Secondary Only Control: PBS was used instead of the primary antibody as the negative control.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SHP1 antibody [EPR5519] (ab124942)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SHP1 antibody [EPR5519] (ab124942)
      ab124942, at a 1/1000 dilution, staining SHP1 in paraffin-embedded Human tonsil tissue by immunohistochemistry
    • Other - Anti-SHP1 antibody [EPR5519] (ab124942)
      Other - Anti-SHP1 antibody [EPR5519] (ab124942)
      Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References

    This product has been referenced in:
    • Hagberg N  et al. Systemic lupus erythematosus immune complexes increase the expression of SLAM family members CD319 (CRACC) and CD229 (LY-9) on plasmacytoid dendritic cells and CD319 on CD56(dim) NK cells. J Immunol 191:2989-98 (2013). Human . Read more (PubMed: 23956418) »

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