Overview

  • Product name

    Anti-SHP1 antibody [M160]
    See all SHP1 primary antibodies
  • Description

    Mouse monoclonal [M160] to SHP1
  • Host species

    Mouse
  • Specificity

    ab76202 does not cross-react with SHP2.
  • Tested applications

    Suitable for: WB, IP, ELISA, ICCmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Recombinant protein containing amino acids in the C terminal region of human SHP1.

  • Positive control

    • Jurkat cells treated with pervanadate (1 mM) for 30 min.

Properties

Applications

Our Abpromise guarantee covers the use of ab76202 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500. Detects a band of approximately 68 kDa (predicted molecular weight: 68 kDa).
IP 1/100.
ELISA 1/2000.
ICC 1/100.

Target

  • Function

    Plays a key role in hematopoiesis. This PTPase activity may directly link growth factor receptors and other signaling proteins through protein-tyrosine phosphorylation. The SH2 regions may interact with other cellular components to modulate its own phosphatase activity against interacting substrates. Together with MTUS1, induces UBE2V2 expression upon angiotensin II stimulation.
  • Tissue specificity

    Isoform 1 is expressed in hematopoietic cells. Isoform 2 is expressed in non-hematopoietic cells.
  • Sequence similarities

    Belongs to the protein-tyrosine phosphatase family. Non-receptor class 2 subfamily.
    Contains 2 SH2 domains.
    Contains 1 tyrosine-protein phosphatase domain.
  • Post-translational
    modifications

    Phosphorylated on serine and tyrosine residues.
  • Cellular localization

    Cytoplasm. Nucleus. In neurons, translocates into the nucleus after treatment with angiotensin II.
  • Information by UniProt
  • Database links

  • Alternative names

    • 70Z-SHP antibody
    • EC 3.1.3.48 antibody
    • HCP antibody
    • HCPH antibody
    • Hematopoietic cell phosphatase antibody
    • Hematopoietic cell protein tyrosine phosphatase antibody
    • Hematopoietic cell protein-tyrosine phosphatase antibody
    • HPTP1C antibody
    • Protein tyrosine phosphatase 1C antibody
    • Protein tyrosine phosphatase non receptor type 6 antibody
    • Protein tyrosine phosphatase SHP1 antibody
    • Protein-tyrosine phosphatase 1C antibody
    • protein-tyrosine phosphatase SHP 1 antibody
    • Protein-tyrosine phosphatase SHP-1 antibody
    • PTN6_HUMAN antibody
    • PTP 1C antibody
    • PTP-1C antibody
    • PTP1C antibody
    • Ptpn6 antibody
    • SH PTP 1 antibody
    • SH PTP1 antibody
    • SH-PTP1 antibody
    • SHP 1 antibody
    • SHP 1L antibody
    • SHP1 antibody
    • SHP1L antibody
    • tyrosine protein phosphatase non receptor type 6 antibody
    • Tyrosine-protein phosphatase non-receptor type 6 antibody
    see all

Images

  • All lanes : Anti-SHP1 antibody [M160] (ab76202) at 1/500 dilution

    Lane 1 : Jurkat cells treated with
    pervanadate (1 mM) for 30 min
    Lane 2 : Jurkat cells treated with
    pervanadate (1 mM) for 30 min and treated with lambda phosphatase

    Predicted band size: 68 kDa
    Observed band size: 68 kDa

References

ab76202 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Western blot
Sample
Mouse Cell lysate - whole cell (Differentiated 3T3-L1 adipocytes)
Gel Running Conditions
Reduced Denaturing (8.5%)
Loading amount
30 µg
Treatment
Transfected with scramble or Shp1 shRNA
Specification
Differentiated 3T3-L1 adipocytes
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C

Abcam user community

Verified customer

Submitted Aug 30 2016

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up