Liver tissue were homogenized with RIPA buffer (RIPA buffer pH 8.6 containing 150 mM NaCI, 1% NP — 40, 0.5% sodium deoxycholate, 0.1% SDS, 50 mM Tris, 5 mM EDTA, 1 mM EGTA, 10 mM sodium fluoride, 20 mM DTT, 1 mM benzamidine HCI, 1mMPMSF, 1 µM aprotinin, pepstatin A and okadaic acid.
All lanes are loaded with rat liver tissue lysates from different high fat diet treatment groups.
Nitrocellulose membrane and CL – Xposure films were from Amersham;
ECL: WestPico Super Signal.
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Submitted Nov 17 2016
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