Western blot abreview for Anti-SHP1 antibody [Y476]

Good
Abreviews
Application
Western blot
Sample
Mouse Cell lysate - whole cell (CD4+ splenocytes)
Loading amount
100000 cells
Specification
CD4+ splenocytes
Treatment
anti-CD3 anti-CD28 antibody-coated beads
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1%

Other product details

Dilution
1/1500
Incubation time
2 hour(s) and 0 minute(s)

Secondary antibody

Detection

Detection method
ECL
Exposure
30 second(s)
Bands
Specific: approx. 68 kDa Non-specific: 160, 50 kDa
Positive control
CD4+ TCR transgenic murine splenocytes

Additional data

Additional Notes
legend for image: TCR transgenic murine CD4+ splenocytes stimulated for 48 h with anti-CD3 and anti-CD28-coated latex beads.

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Submitted Nov 27 2006

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