Overview

  • Product name
    Anti-SHP1 (phospho S591) antibody
    See all SHP1 primary antibodies
  • Description
    Rabbit polyclonal to SHP1 (phospho S591)
  • Host species
    Rabbit
  • Specificity
    Phospho-Ser591-SHP1 specific. This antibody detects a 68 kDa band on SDS-PAGE immunoblots of human Jurkat and A431 cells treated with pervanadate, as well as A431 cells treated with EGF.
  • Tested applications
    Suitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic phospho-Ser591-SHP1 peptide (coupled to carrier protein) corresponding to amino acids around serine 591 in human SHP1. The sequence is highly conserved in rat and mouse SHP1.

Properties

Applications

Our Abpromise guarantee covers the use of ab41436 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 68 kDa (predicted molecular weight: 68 kDa).
ELISA 1/2000.

Target

  • Function
    Plays a key role in hematopoiesis. This PTPase activity may directly link growth factor receptors and other signaling proteins through protein-tyrosine phosphorylation. The SH2 regions may interact with other cellular components to modulate its own phosphatase activity against interacting substrates. Together with MTUS1, induces UBE2V2 expression upon angiotensin II stimulation.
  • Tissue specificity
    Isoform 1 is expressed in hematopoietic cells. Isoform 2 is expressed in non-hematopoietic cells.
  • Sequence similarities
    Belongs to the protein-tyrosine phosphatase family. Non-receptor class 2 subfamily.
    Contains 2 SH2 domains.
    Contains 1 tyrosine-protein phosphatase domain.
  • Post-translational
    modifications
    Phosphorylated on serine and tyrosine residues.
  • Cellular localization
    Cytoplasm. Nucleus. In neurons, translocates into the nucleus after treatment with angiotensin II.
  • Information by UniProt
  • Database links
  • Alternative names
    • 70Z-SHP antibody
    • EC 3.1.3.48 antibody
    • HCP antibody
    • HCPH antibody
    • Hematopoietic cell phosphatase antibody
    • Hematopoietic cell protein tyrosine phosphatase antibody
    • Hematopoietic cell protein-tyrosine phosphatase antibody
    • HPTP1C antibody
    • Protein tyrosine phosphatase 1C antibody
    • Protein tyrosine phosphatase non receptor type 6 antibody
    • Protein tyrosine phosphatase SHP1 antibody
    • Protein-tyrosine phosphatase 1C antibody
    • protein-tyrosine phosphatase SHP 1 antibody
    • Protein-tyrosine phosphatase SHP-1 antibody
    • PTN6_HUMAN antibody
    • PTP 1C antibody
    • PTP-1C antibody
    • PTP1C antibody
    • Ptpn6 antibody
    • SH PTP 1 antibody
    • SH PTP1 antibody
    • SH-PTP1 antibody
    • SHP 1 antibody
    • SHP 1L antibody
    • SHP1 antibody
    • SHP1L antibody
    • tyrosine protein phosphatase non receptor type 6 antibody
    • Tyrosine-protein phosphatase non-receptor type 6 antibody
    see all

Images

  • Lanes 1-2 : anti-SHP1 (C-terminal)

    Lanes 3-6 : Anti-SHP1 (phospho S591) antibody (ab41436) at 1/1000 dilution

    Lanes 1 & 3 : human Jurkat cells treated with 1 mM pervanadate for 30 min.
    Lanes 2 & 4 : human Jurkat cells treated with 1 mM pervanadate for 30 min, blot exposed to lambda phosphatase before probing

    Lane 5 : human Jurkat cells treated with 1 mM pervanadate for 30 min. with phospho-Ser591-SHP1 peptide

    Lane 6 : human Jurkat cells treated with 1 mM pervanadate for 30 min. with non specific phospho-serine peptide

    Predicted band size: 68 kDa
    Observed band size: 68 kDa



    Membrane was incubated with diluted antibody in 5% non-fat milk, PBS, 0.04% Tween20 for 1 hour at room temperature.

References

This product has been referenced in:
  • Mkaddem SB  et al. Lyn and Fyn function as molecular switches that control immunoreceptors to direct homeostasis or inflammation. Nat Commun 8:246 (2017). Read more (PubMed: 28811476) »
See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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