Key features and details
- Rabbit polyclonal to SHP1 (phospho S591)
- Suitable for: WB, ELISA
- Reacts with: Human
- Isotype: IgG
Product nameAnti-SHP1 (phospho S591) antibody
See all SHP1 primary antibodies
DescriptionRabbit polyclonal to SHP1 (phospho S591)
SpecificityPhospho-Ser591-SHP1 specific. This antibody detects a 68 kDa band on SDS-PAGE immunoblots of human Jurkat and A431 cells treated with pervanadate, as well as A431 cells treated with EGF.
Tested applicationsSuitable for: WB, ELISAmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat
Synthetic phospho-Ser591-SHP1 peptide (coupled to carrier protein) corresponding to amino acids around serine 591 in human SHP1. The sequence is highly conserved in rat and mouse SHP1.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.05% Sodium azide
Constituents: PBS, 50% Glycerol, 0.1% BSA
Concentration information loading...
PurityImmunogen affinity purified
Purification notesThis antibody was cross-adsorbed to a non specific phospho-serine peptide then affinity purified using phospho-Ser591-SHP1 peptide (immunogen).
Our Abpromise guarantee covers the use of ab41436 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000. Detects a band of approximately 68 kDa (predicted molecular weight: 68 kDa).|
FunctionPlays a key role in hematopoiesis. This PTPase activity may directly link growth factor receptors and other signaling proteins through protein-tyrosine phosphorylation. The SH2 regions may interact with other cellular components to modulate its own phosphatase activity against interacting substrates. Together with MTUS1, induces UBE2V2 expression upon angiotensin II stimulation.
Tissue specificityIsoform 1 is expressed in hematopoietic cells. Isoform 2 is expressed in non-hematopoietic cells.
Sequence similaritiesBelongs to the protein-tyrosine phosphatase family. Non-receptor class 2 subfamily.
Contains 2 SH2 domains.
Contains 1 tyrosine-protein phosphatase domain.
modificationsPhosphorylated on serine and tyrosine residues.
Cellular localizationCytoplasm. Nucleus. In neurons, translocates into the nucleus after treatment with angiotensin II.
- Information by UniProt
- 70Z-SHP antibody
- EC 188.8.131.52 antibody
- HCP antibody
Lanes 1-2 : anti-SHP1 (C-terminal)
Lanes 3-6 : Anti-SHP1 (phospho S591) antibody (ab41436) at 1/1000 dilution
Lanes 1 & 3 : human Jurkat cells treated with 1 mM pervanadate for 30 min.
Lanes 2 & 4 : human Jurkat cells treated with 1 mM pervanadate for 30 min, blot exposed to lambda phosphatase before probing
Lane 5 : human Jurkat cells treated with 1 mM pervanadate for 30 min. with phospho-Ser591-SHP1 peptide
Lane 6 : human Jurkat cells treated with 1 mM pervanadate for 30 min. with non specific phospho-serine peptide
Predicted band size: 68 kDa
Observed band size: 68 kDa
Membrane was incubated with diluted antibody in 5% non-fat milk, PBS, 0.04% Tween20 for 1 hour at room temperature.
ab41436 has been referenced in 1 publication.
- Mkaddem SB et al. Lyn and Fyn function as molecular switches that control immunoreceptors to direct homeostasis or inflammation. Nat Commun 8:246 (2017). PubMed: 28811476