Overview

  • Product name

    Anti-SHP1 (phospho Y536) antibody
    See all SHP1 primary antibodies
  • Description

    Rabbit polyclonal to SHP1 (phospho Y536)
  • Host species

    Rabbit
  • Specificity

    ab131500 detects endogenous levels of SHP1 only when phosphorylated at tyrosine 536.
  • Tested applications

    Suitable for: IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic phosphopeptide conjugated to KLH, derived from around the phosphorylation site of tyrosine 536 (SEYpGN) of Human SHP1 (NP_002822.2).

  • Positive control

    • HeLa cells; Human breast carcinoma tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab131500 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/100.
ICC/IF 1/100 - 1/200.

Target

  • Function

    Plays a key role in hematopoiesis. This PTPase activity may directly link growth factor receptors and other signaling proteins through protein-tyrosine phosphorylation. The SH2 regions may interact with other cellular components to modulate its own phosphatase activity against interacting substrates. Together with MTUS1, induces UBE2V2 expression upon angiotensin II stimulation.
  • Tissue specificity

    Isoform 1 is expressed in hematopoietic cells. Isoform 2 is expressed in non-hematopoietic cells.
  • Sequence similarities

    Belongs to the protein-tyrosine phosphatase family. Non-receptor class 2 subfamily.
    Contains 2 SH2 domains.
    Contains 1 tyrosine-protein phosphatase domain.
  • Post-translational
    modifications

    Phosphorylated on serine and tyrosine residues.
  • Cellular localization

    Cytoplasm. Nucleus. In neurons, translocates into the nucleus after treatment with angiotensin II.
  • Information by UniProt
  • Database links

  • Alternative names

    • 70Z-SHP antibody
    • EC 3.1.3.48 antibody
    • HCP antibody
    • HCPH antibody
    • Hematopoietic cell phosphatase antibody
    • Hematopoietic cell protein tyrosine phosphatase antibody
    • Hematopoietic cell protein-tyrosine phosphatase antibody
    • HPTP1C antibody
    • Protein tyrosine phosphatase 1C antibody
    • Protein tyrosine phosphatase non receptor type 6 antibody
    • Protein tyrosine phosphatase SHP1 antibody
    • Protein-tyrosine phosphatase 1C antibody
    • protein-tyrosine phosphatase SHP 1 antibody
    • Protein-tyrosine phosphatase SHP-1 antibody
    • PTN6_HUMAN antibody
    • PTP 1C antibody
    • PTP-1C antibody
    • PTP1C antibody
    • Ptpn6 antibody
    • SH PTP 1 antibody
    • SH PTP1 antibody
    • SH-PTP1 antibody
    • SHP 1 antibody
    • SHP 1L antibody
    • SHP1 antibody
    • SHP1L antibody
    • tyrosine protein phosphatase non receptor type 6 antibody
    • Tyrosine-protein phosphatase non-receptor type 6 antibody
    see all

Images

  • Immunofluorescent analysis of methanol-fixed HeLa cells labelling SHP1 (phospho Y536) with ab131500 at 1/100 dilution.
  • Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue labelling SHP1 (phospho Y536) with ab131500 at 1/50 dilution (left) or ab131500 preincubated with blocking peptide (right).

References

ab131500 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Answer

Thank you foryour reply.

The buffers for these product do not contain stabilizing proteins.



Ab51171: Preservative: 0.02% Sodium Azide

Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4



ab131500: Preservative: 0.02% Sodium azide

Constituents: 49% PBS, 50% Glycerol, 0.88% Sodium chloride, pH: 7.4

Note: PBS without Mg2+ and Ca2+



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