Product nameAnti-SHP1 (phospho Y536) antibody
See all SHP1 primary antibodies
DescriptionRabbit polyclonal to SHP1 (phospho Y536)
SpecificityPhospho-Tyr536-SHP1 specific. The antibody detects a 68 kDa band on SDS-PAGE immunoblots of human Jurkat cells treated with pervanadate (not observed in control cells).
Tested applicationsSuitable for: WB, ELISA, ICC/IFmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat
Synthetic phospho-Tyr536-SHP1 peptide (coupled to carrier protein) corresponding to amino acids around tyrosine 536 in human SHP1. The sequence is highly conserved in rat and mouse SHP1.
- This antibody gave a positive result when used in the following methanol fixed cell lines: HepG2
Storage instructionsShipped at 4°C. Store at 4°C (up to 6 months). Store at -20°C.
Storage bufferPreservative: 0.05% Sodium azide
Constituents: PBS, 50% Glycerol, 0.1% BSA
Concentration information loading...
PurityImmunogen affinity purified
Purification notesThis antibody was cross-adsorbed to a non specific phospho-tyrosine peptide then affinity purified using phospho-Tyr536-SHP1 peptide (immunogen).
Our Abpromise guarantee covers the use of ab41437 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000. Detects a band of approximately 68 kDa (predicted molecular weight: 68 kDa).|
FunctionPlays a key role in hematopoiesis. This PTPase activity may directly link growth factor receptors and other signaling proteins through protein-tyrosine phosphorylation. The SH2 regions may interact with other cellular components to modulate its own phosphatase activity against interacting substrates. Together with MTUS1, induces UBE2V2 expression upon angiotensin II stimulation.
Tissue specificityIsoform 1 is expressed in hematopoietic cells. Isoform 2 is expressed in non-hematopoietic cells.
Sequence similaritiesBelongs to the protein-tyrosine phosphatase family. Non-receptor class 2 subfamily.
Contains 2 SH2 domains.
Contains 1 tyrosine-protein phosphatase domain.
modificationsPhosphorylated on serine and tyrosine residues.
Cellular localizationCytoplasm. Nucleus. In neurons, translocates into the nucleus after treatment with angiotensin II.
- Information by UniProt
- 70Z-SHP antibody
- EC 18.104.22.168 antibody
- HCP antibody
ICC/IF image of ab41437 stained HepG2 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab41437 at 1/200 dilution overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Lanes 1-2 : anti-SHP1 (C-terminal)
Lanes 3-5 : Anti-SHP1 (phospho Y536) antibody (ab41437) at 1/1000 dilution
Lanes 1 & 3 : human Jurkat cells treated with 1 mM pervanadate for 30 min.
Lanes 2 & 4 : human Jurkat cells treated with 1 mM pervanadate for 30 min, blot exposed to alkaline phosphatase before probing
Lane 5 : human Jurkat cells treated with 1 mM pervanadate for 30 min.
with phospho-Tyr536-SHP1 peptide
Predicted band size: 68 kDa
Observed band size: 68 kDa
Membrane was incubated with diluted antibody in 5% non-fat milk, PBS, 0.04% Tween20 for 1 hour at room temperature.
This product has been referenced in:
- Zhou X et al. Leishmania infantum-chagasi activates SHP-1 and reduces NFAT5/TonEBP activity in the mouse kidney inner medulla. Am J Physiol Renal Physiol 307:F516-24 (2014). Read more (PubMed: 24990897) »
- Kim KA et al. Calpain-dependent cleavage of SHP-1 and SHP-2 is involved in the dephosphorylation of Jurkat T cells induced by Entamoeba histolytica. Parasite Immunol 32:176-83 (2010). WB ; Human . Read more (PubMed: 20398180) »