Overview

  • Product name
    Anti-SHP1 (phospho Y536) antibody
    See all SHP1 primary antibodies
  • Description
    Rabbit polyclonal to SHP1 (phospho Y536)
  • Host species
    Rabbit
  • Specificity
    ab51171 detects endogenous levels of SHP1 only when phosphorylated at tyrosine 536.
  • Tested applications
    Suitable for: ELISA, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic phosphopeptide derived from human SHP1 around the phosphorylation site of tyrosine 536 (S-E-YP-G-N).

  • Positive control
    • Human breast carcinoma tissue and RAW264.7 cell extracts treated with EGF (200ng/ml, 5min).

Properties

Applications

Our Abpromise guarantee covers the use of ab51171 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/10000.
IHC-P Use at an assay dependent concentration.
WB 1/300 - 1/1000. Predicted molecular weight: 68 kDa.

Target

  • Function
    Plays a key role in hematopoiesis. This PTPase activity may directly link growth factor receptors and other signaling proteins through protein-tyrosine phosphorylation. The SH2 regions may interact with other cellular components to modulate its own phosphatase activity against interacting substrates. Together with MTUS1, induces UBE2V2 expression upon angiotensin II stimulation.
  • Tissue specificity
    Isoform 1 is expressed in hematopoietic cells. Isoform 2 is expressed in non-hematopoietic cells.
  • Sequence similarities
    Belongs to the protein-tyrosine phosphatase family. Non-receptor class 2 subfamily.
    Contains 2 SH2 domains.
    Contains 1 tyrosine-protein phosphatase domain.
  • Post-translational
    modifications
    Phosphorylated on serine and tyrosine residues.
  • Cellular localization
    Cytoplasm. Nucleus. In neurons, translocates into the nucleus after treatment with angiotensin II.
  • Information by UniProt
  • Database links
  • Alternative names
    • 70Z-SHP antibody
    • EC 3.1.3.48 antibody
    • HCP antibody
    • HCPH antibody
    • Hematopoietic cell phosphatase antibody
    • Hematopoietic cell protein tyrosine phosphatase antibody
    • Hematopoietic cell protein-tyrosine phosphatase antibody
    • HPTP1C antibody
    • Protein tyrosine phosphatase 1C antibody
    • Protein tyrosine phosphatase non receptor type 6 antibody
    • Protein tyrosine phosphatase SHP1 antibody
    • Protein-tyrosine phosphatase 1C antibody
    • protein-tyrosine phosphatase SHP 1 antibody
    • Protein-tyrosine phosphatase SHP-1 antibody
    • PTN6_HUMAN antibody
    • PTP 1C antibody
    • PTP-1C antibody
    • PTP1C antibody
    • Ptpn6 antibody
    • SH PTP 1 antibody
    • SH PTP1 antibody
    • SH-PTP1 antibody
    • SHP 1 antibody
    • SHP 1L antibody
    • SHP1 antibody
    • SHP1L antibody
    • tyrosine protein phosphatase non receptor type 6 antibody
    • Tyrosine-protein phosphatase non-receptor type 6 antibody
    see all

Images

  • ab51171 at 1/50 dilution staining SHP1 in human breast carcinoma by Immunohistochemistry, Paraffin embedded tissue, in the absence or presence of the immunising peptide.
  • Western blot analysis of extracts from RAW264.7 cells treated with EGF (200ng/ml, 5 minutes) Western blot analysis of extracts from RAW264.7 cells treated with EGF (200ng/ml, 5 minutes)

References

This product has been referenced in:
  • Savola P  et al. Somatic mutations in clonally expanded cytotoxic T lymphocytes in patients with newly diagnosed rheumatoid arthritis. Nat Commun 8:15869 (2017). Read more (PubMed: 28635960) »
  • Chen G  et al. PD-L1 inhibits acute and chronic pain by suppressing nociceptive neuron activity via PD-1. Nat Neurosci 20:917-926 (2017). Read more (PubMed: 28530662) »
See all 7 Publications for this product

Customer reviews and Q&As

1-4 of 4 Abreviews or Q&A

Application
Flow Cytometry
Sample
Human Cell (Jurkat)
Specification
Jurkat
Preparation
Cell harvesting/tissue preparation method: human Jurkat cells treated with pervanadate (1 mM) for 30 min
Sample buffer: complete RPMI media
Fixation
Paraformaldehyde
Permeabilization
Yes - methanol
Gating Strategy
Jurkat

Abcam user community

Verified customer

Submitted Feb 18 2013

Answer

Thank you foryour reply.

The buffers for these product do not contain stabilizing proteins.



Ab51171: Preservative: 0.02% Sodium Azide

Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4



ab131500: Preservative: 0.02% Sodium azide

Constituents: 49% PBS, 50% Glycerol, 0.88% Sodium chloride, pH: 7.4

Note: PBS without Mg2+ and Ca2+



I am very happy to hear that you would like to participate in the AbTrial program. The codes which are issues corespond to the cost of the antibody tested, so for ab51171 the code would have a value of $337. These codes expire four months after they are dreated. Would you like me to issue codes for both of these products now or would you like to test one and then after that, test the other? As we have already spoken about the codes, you do not need to wait for these to arrive before making your order.

You can receive any RabMAb free with order of a primary antibody this week. Please follow the banner ad on our website for more information.


I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Free Rabbit monoclonal antibody with any purchase of a primary antibody, while stocks last! Quote “RABMAB-XBSMG” in your next primary antibody order. For more information, visit the following link: https://www.abcam.com/index.html?pageconfig=resource&rid=15447

Read More

Answer

Thank you for your reply.

Unfortunately none of these products has yet been tested in flow cytometry. While we do try and test as many products as we can in a large number of applications, it does seem as this group has not yet been though FC tests.

In regard to your sample size inquiry, we do not currently offer small or sample sizes for our products. This is partly a matter of logistics as we now have over 100,000 products on the catalog but is also because of our Abpromise guarantee in which we offer support, refund or replacement for any product which does not perform as stated on the datasheet. While the anti-SHP1 phospho specific antibodies would not be covered in flow cytometry by that guarantee, we do offer the AbTrial program instead.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Free Rabbit monoclonal antibody with any purchase of a primary antibody, while stocks last! Quote “RABMAB-XBSMG” in your next primary antibody order. For more information, visit the following link: https://www.abcam.com/index.html?pageconfig=resource&rid=15447

Read More

Question
Answer

Thank you for your phone call. As I mentioned, because these are phospho-specific antibodies, we recommend using BSA as a blocking agent instead of milk. If this does not help to improve your results, please let me knowyour original order or PO numberand I will be happy to offer you a free of charge replacement with the antibody of your choice.

Read More

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