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ab47282 and ab51171 give no signal in WB on human cells.
Asked on Sep 14 2012
Thank you for your phone call. As I mentioned, because these are phospho-specific antibodies, we recommend using BSA as a blocking agent instead of milk. If this does not help to improve your results, please let me knowyour original order or PO numberand I will be happy to offer you a free of charge replacement with the antibody of your choice.
Answered on Sep 14 2012