Key features and details
- Rabbit polyclonal to SHP1 (phospho Y564)
- Suitable for: WB, ELISA
- Reacts with: Human
- Isotype: IgG
Product nameAnti-SHP1 (phospho Y564) antibody
See all SHP1 primary antibodies
DescriptionRabbit polyclonal to SHP1 (phospho Y564)
Specificityab79294 detects endogenous levels of SHP1 only when phosphorylated at tyrosine 564.
Tested applicationsSuitable for: WB, ELISAmore details
Species reactivityReacts with: Human
Syntheticphosphopeptide derived from human SHP1 around the phosphorylation site of tyrosine 564 (DVYPEN).
- Extracts from COS7 cells, treated with EGF (200ng/ml, 30mins)
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol, 0.87% Sodium chloride
Without Mg2+ and Ca2+
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab79294 is purified from rabbit antiserum by chromatography using an epitope specific phosphopeptide. The antibody against non phosphopeptide was removed by chromatography using non phosphopeptide corresponding to the phosphorylation site.
Our Abpromise guarantee covers the use of ab79294 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/1000. Predicted molecular weight: 68 kDa.|
FunctionPlays a key role in hematopoiesis. This PTPase activity may directly link growth factor receptors and other signaling proteins through protein-tyrosine phosphorylation. The SH2 regions may interact with other cellular components to modulate its own phosphatase activity against interacting substrates. Together with MTUS1, induces UBE2V2 expression upon angiotensin II stimulation.
Tissue specificityIsoform 1 is expressed in hematopoietic cells. Isoform 2 is expressed in non-hematopoietic cells.
Sequence similaritiesBelongs to the protein-tyrosine phosphatase family. Non-receptor class 2 subfamily.
Contains 2 SH2 domains.
Contains 1 tyrosine-protein phosphatase domain.
modificationsPhosphorylated on serine and tyrosine residues.
Cellular localizationCytoplasm. Nucleus. In neurons, translocates into the nucleus after treatment with angiotensin II.
- Information by UniProt
- 70Z-SHP antibody
- EC 188.8.131.52 antibody
- HCP antibody
All lanes : Anti-SHP1 (phospho Y564) antibody (ab79294) at 1/1000 dilution
Lane 1 : extracts from COS7 cells, treated with EGF (200 ng/ml, 30 mins)
Lane 2 : extracts from COS7 cells, treated with EGF (200 ng/ml, 30 mins) with immunising peptide at 10 µg
Lysates/proteins at 30 µg per lane.
Predicted band size: 68 kDa
ab79294 has not yet been referenced specifically in any publications.