Key features and details
- Mouse monoclonal [M163] to SHP2
- Suitable for: WB
- Knockout validated
- Reacts with: Mouse, Human
- Isotype: IgG1
Product nameAnti-SHP2 antibody [M163]
See all SHP2 primary antibodies
DescriptionMouse monoclonal [M163] to SHP2
Specificityab76285 does not cross-react with SHP1.
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat
Recombinant fragment corresponding to Human SHP2 (N terminal).
- Human A431 and Jurkat cells, and adult mouse brain lysates.
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferPreservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 50% Glycerol, PBS
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab76285 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000. Detects a band of approximately 72 kDa (predicted molecular weight: 68 kDa).|
FunctionActs downstream of various receptor and cytoplasmic protein tyrosine kinases to participate in the signal transduction from the cell surface to the nucleus.
Tissue specificityWidely expressed, with highest levels in heart, brain, and skeletal muscle.
Involvement in diseaseDefects in PTPN11 are the cause of LEOPARD syndrome type 1 (LEOPARD1) [MIM:151100]. It is an autosomal dominant disorder allelic with Noonan syndrome. The acronym LEOPARD stands for lentigines, electrocardiographic conduction abnormalities, ocular hypertelorism, pulmonic stenosis, abnormalities of genitalia, retardation of growth, and deafness.
Defects in PTPN11 are the cause of Noonan syndrome type 1 (NS1) [MIM:163950]. Noonan syndrome (NS) is a disorder characterized by dysmorphic facial features, short stature, hypertelorism, cardiac anomalies, deafness, motor delay, and a bleeding diathesis. Some patients with Noonan syndrome type 1 develop multiple giant cell lesions of the jaw or other bony or soft tissues, which are classified as pigmented villomoduolar synovitis (PVNS) when occurring in the jaw or joints. Note=Mutations in PTPN11 account for more than 50% of the cases. Rarely, NS is associated with juvenile myelomonocytic leukemia (JMML). NS1 inheritance is autosomal dominant.
Defects in PTPN11 are a cause of juvenile myelomonocytic leukemia (JMML) [MIM:607785]. JMML is a pediatric myelodysplastic syndrome that constitutes approximately 30% of childhood cases of myelodysplastic syndrome (MDS) and 2% of leukemia. It is characterized by leukocytosis with tissue infiltration and in vitro hypersensitivity of myeloid progenitors to granulocyte-macrophage colony stimulating factor.
Defects in PTPN11 are a cause of metachondromatosis (MC) [MIM:156250]. It is a skeletal disorder with radiologic fetarures of both multiple exostoses and Ollier disease, characterized by the presence of multiple enchondromas and osteochondroma-like lesions.
Sequence similaritiesBelongs to the protein-tyrosine phosphatase family. Non-receptor class 2 subfamily.
Contains 2 SH2 domains.
Contains 1 tyrosine-protein phosphatase domain.
DomainThe SH2 domains repress phosphatase activity. Binding of these domains to phosphotyrosine-containing proteins relieves this auto-inhibition, possibly by inducing a conformational change in the enzyme.
modificationsPhosphorylated on Tyr-546 and Tyr-584 upon receptor protein tyrosine kinase activation; which creates a binding site for GRB2 and other SH2-containing proteins.
- Information by UniProt
- BPTP3 antibody
- CFC antibody
- JMML antibody
Lane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: SHP2 knockout HAP1 cell lysate (20 µg)
Lane 3: A431 cell lysate (20 µg)
Lane 4: Jurkat cell lysate (20 µg)
Lanes 1 to 4: Merged signal (red and green). Green - ab76285 observed at 68 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab76285 was shown to specifically react with SHP2 when SHP2 knockout samples were used. Wild-type and SHP2 knockout samples were subjected to SDS-PAGE. ab76285 and ab8245 (loading control to GAPDH) were both diluted 1/1000 and 1/10000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10000 dilution for 1 h at room temperature before imaging.
Lane 1 : Anti-SHP2 antibody [M163] (ab76285) at 1/250 dilution
Lane 2 : Anti-SHP2 antibody [M163] (ab76285) at 1/500 dilution
Lane 3 : Anti-SHP2 antibody [M163] (ab76285) at 1/1000 dilution
Lane 4 : Anti-SHP2 antibody [M163] (ab76285) at 1/2000 dilution
All lanes : adult mouse brain lysates
Predicted band size: 68 kDa
Observed band size: 72 kDa why is the actual band size different from the predicted?
ab76285 has been referenced in 3 publications.
- Wang S et al. YB1 protects cardiac myocytes against H2O2-induced injury via suppression of PIAS3 mRNA and phosphorylation of STAT3. Mol Med Rep 19:4579-4588 (2019). PubMed: 30942400
- Bowen ME et al. SHP2 regulates chondrocyte terminal differentiation, growth plate architecture and skeletal cell fates. PLoS Genet 10:e1004364 (2014). WB ; Mouse . PubMed: 24875294
- Kikkawa N et al. miR-489 is a tumour-suppressive miRNA target PTPN11 in hypopharyngeal squamous cell carcinoma (HSCC). Br J Cancer 103:877-84 (2010). WB ; Human . PubMed: 20700123