Anti-SHP2 (phospho Y542) antibody [EP508(2)Y] (ab62322)


  • Product name
    Anti-SHP2 (phospho Y542) antibody [EP508(2)Y]
    See all SHP2 primary antibodies
  • Description
    Rabbit monoclonal [EP508(2)Y] to SHP2 (phospho Y542)
  • Host species
  • Specificity
    ab62322 detects SHP2 phosphorylated at Tyrosine 542.
  • Tested applications
    Suitable for: ICC/IF, WB, IPmore details
    Unsuitable for: Flow Cyt
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic phosphopeptide corresponding to residues surrounding Tyrosine 542 of human SHP2.

  • Positive control
    • NIH/3T3 cell lysate.
  • General notes

    Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.


    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.



Our Abpromise guarantee covers the use of ab62322 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/100 - 1/250.
WB 1/50000 - 1/100000. Detects a band of approximately 68 kDa (predicted molecular weight: 68 kDa).
IP 1/40.
  • Application notes
    Is unsuitable for Flow Cyt.
  • Target

    • Function
      Acts downstream of various receptor and cytoplasmic protein tyrosine kinases to participate in the signal transduction from the cell surface to the nucleus.
    • Tissue specificity
      Widely expressed, with highest levels in heart, brain, and skeletal muscle.
    • Involvement in disease
      Defects in PTPN11 are the cause of LEOPARD syndrome type 1 (LEOPARD1) [MIM:151100]. It is an autosomal dominant disorder allelic with Noonan syndrome. The acronym LEOPARD stands for lentigines, electrocardiographic conduction abnormalities, ocular hypertelorism, pulmonic stenosis, abnormalities of genitalia, retardation of growth, and deafness.
      Defects in PTPN11 are the cause of Noonan syndrome type 1 (NS1) [MIM:163950]. Noonan syndrome (NS) is a disorder characterized by dysmorphic facial features, short stature, hypertelorism, cardiac anomalies, deafness, motor delay, and a bleeding diathesis. Some patients with Noonan syndrome type 1 develop multiple giant cell lesions of the jaw or other bony or soft tissues, which are classified as pigmented villomoduolar synovitis (PVNS) when occurring in the jaw or joints. Note=Mutations in PTPN11 account for more than 50% of the cases. Rarely, NS is associated with juvenile myelomonocytic leukemia (JMML). NS1 inheritance is autosomal dominant.
      Defects in PTPN11 are a cause of juvenile myelomonocytic leukemia (JMML) [MIM:607785]. JMML is a pediatric myelodysplastic syndrome that constitutes approximately 30% of childhood cases of myelodysplastic syndrome (MDS) and 2% of leukemia. It is characterized by leukocytosis with tissue infiltration and in vitro hypersensitivity of myeloid progenitors to granulocyte-macrophage colony stimulating factor.
      Defects in PTPN11 are a cause of metachondromatosis (MC) [MIM:156250]. It is a skeletal disorder with radiologic fetarures of both multiple exostoses and Ollier disease, characterized by the presence of multiple enchondromas and osteochondroma-like lesions.
    • Sequence similarities
      Belongs to the protein-tyrosine phosphatase family. Non-receptor class 2 subfamily.
      Contains 2 SH2 domains.
      Contains 1 tyrosine-protein phosphatase domain.
    • Domain
      The SH2 domains repress phosphatase activity. Binding of these domains to phosphotyrosine-containing proteins relieves this auto-inhibition, possibly by inducing a conformational change in the enzyme.
    • Post-translational
      Phosphorylated on Tyr-546 and Tyr-584 upon receptor protein tyrosine kinase activation; which creates a binding site for GRB2 and other SH2-containing proteins.
    • Cellular localization
    • Information by UniProt
    • Database links
    • Alternative names
      • BPTP3 antibody
      • CFC antibody
      • JMML antibody
      • METCDS antibody
      • MGC14433 antibody
      • NS1 antibody
      • OTTHUMP00000166107 antibody
      • OTTHUMP00000166108 antibody
      • Protein tyrosine phosphatase 2 antibody
      • Protein tyrosine phosphatase 2C antibody
      • Protein tyrosine phosphatase non receptor type 11 antibody
      • Protein-tyrosine phosphatase 1D antibody
      • Protein-tyrosine phosphatase 2C antibody
      • PTN11_HUMAN antibody
      • PTP-1D antibody
      • PTP-2C antibody
      • PTP1D antibody
      • PTP2C antibody
      • PTPN11 antibody
      • SAP2 antibody
      • SH-PTP2 antibody
      • SH-PTP3 antibody
      • SH2 domain containing protein tyrosine phosphatase 2 antibody
      • SHP 2 antibody
      • SHP-2 antibody
      • Shp2 antibody
      • SHPTP2 antibody
      • SHPTP3 antibody
      • Syp antibody
      • Tyrosine-protein phosphatase non-receptor type 11 antibody
      see all


    • Ab62322 staining SHP2 in THP-1 cells ( Human monocytic leukaemia cell line) by ICC/IF (immunocytochemistry/immunofluorescence). Cells were fixed with 4% Paraformaldehyde and permeabilized with 0.1% TritonX-100. Samples were incubated with purified ab62322 at 8.8 μg/ml. Secondary antibody used was AlexaFluor®488 Goat anti-Rabbit (ab150077) at 2 μg/ml. Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594)( ab195889) used as counterstain at 2.5 μg/ml . DAPI was used as nuclear counterstain. Confocal image showing the expression was increased after treatment with TPA 200nM for 24h and PDGF 50ng/ml for 30min, the signal decreased after treatment with Lambda Protein Phosphatase 31℃ for 2h.

    • All lanes : Anti-SHP2 (phospho Y542) antibody [EP508(2)Y] (ab62322) at 1/5000 dilution

      Lane 1 : THP-1 (Human monocytic leukemia monocyte) whole cell lysates
      Lane 2 : THP-1 (Human monocytic leukemia monocyte) treated with Phorbol-12-myristate-13-acetate and platelet-derived growth factor.
      Lane 3 : THP-1 (Human monocytic leukemia monocyte) treated with Phorbol-12-myristate-13-acetate and platelet-derived growth factor.Then the membrane was incubated with phosphatase.

      Lysates/proteins at 15 µg per lane.

      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 68 kDa
      Observed band size: 68 kDa

      Blocking and diluting buffer used was 5% NFDM/TBST.

      Purified batch of ab62322 was used.

    • All lanes : Anti-SHP2 (phospho Y542) antibody [EP508(2)Y] (ab62322) at 1/1000 dilution

      Lane 1 : SHP2 (pY542) phospho peptide
      Lane 2 : SHP2 non-phospho peptide

      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

      Predicted band size: 68 kDa

      Exposure time: 3 minutes

      Blocking and diluting buffer used was 5% NFDM /TBST.

      Purified ab62322 was used.

    • All lanes : Anti-SHP2 (phospho Y542) antibody [EP508(2)Y] (ab62322) at 1/50000 dilution

      Lane 1 : NIH/3T3 cell lysates; untreated
      Lane 2 : NIH/3T3 cell lysates; treated with PDGF

      Lysates/proteins at 10 µg per lane.

      All lanes : goat anti-rabbit HRP conjugated, at 1/2000 dilution

      Predicted band size: 68 kDa
      Observed band size: 68 kDa

      Beta Tubulin has been included as a loading control.


    This product has been referenced in:
    • Klaesson A  et al. Improved efficiency of in situ protein analysis by proximity ligation using UnFold probes. Sci Rep 8:5400 (2018). Read more (PubMed: 29599435) »
    • Guo W  et al. Tyrosine phosphatase SHP2 negatively regulates NLRP3 inflammasome activation via ANT1-dependent mitochondrial homeostasis. Nat Commun 8:2168 (2017). Read more (PubMed: 29255148) »

    See all 5 Publications for this product

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