Western blot abreview for Anti-SHPRH antibody

Excellent
Abreviews
Application
Western blot
Loading amount
50 µg
Gel Running Conditions
Reduced Denaturing (10%)
Sample
Human Cell lysate - whole cell (HEK293)
Specification
HEK293
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C

Other product details

Incubation time
18 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 1x TBS-T + 3% BSA
Dilution
1/1000

Secondary antibody

Name
Non-Abcam antibody was used: Anti Rabbit HRP
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/10000

Detection

Detection method
ECL+
Bands
Specific: 175 kDa
Exposure
5 minute(s) and 0 second(s)
Positive control
n/A

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Submitted Jan 30 2014

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