Western blot abreview for Anti-SHPRH antibody

Good
Abreviews
Application
Western blot
Sample
Human Cell lysate - whole cell (HCT116 cell lines)
Gel Running Conditions
Reduced Denaturing (4-12%)
Loading amount
30 µg
Specification
HCT116 cell lines
Blocking step
Milk as blocking agent for 45 minute(s) · Concentration: 2% · Temperature: 37°C

Other product details

Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C
Dilution
1/1000

Secondary antibody

Dilution
1/2000
Name
Non-Abcam antibody was used: goat anti rabbit
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase

Detection

Detection method
ecl
Exposure
30 second(s)
Bands
Specific: ~175-180 kDa Non-specific: ~100 kDa

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Submitted Jul 14 2015

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