Overview

  • Product name

    Anti-Shroom 3 antibody
  • Description

    Rabbit polyclonal to Shroom 3
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment, corresponding to amino acids 1413-1509 of Human Shroom 3 (UniProt Q8TF72).

  • Positive control

    • Human rectum tissue.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer

    pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 59% PBS, 40% Glycerol
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab151009 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    Controls cell shape changes in the neuroepithelium during neural tube closure. Induces apical constriction in epithelial cells by promoting the apical accumulation of F-actin and myosin II, and probably by bundling stress fibers. Induces apicobasal cell elongation by redistributing gamma-tubulin and directing the assembly of robust apicobasal microtubule arrays.
  • Sequence similarities

    Belongs to the shroom family.
    Contains 1 ASD1 domain.
    Contains 1 ASD2 domain.
    Contains 1 PDZ (DHR) domain.
  • Domain

    The ASD1 domain mediates F-actin binding.
    The ASD2 domain is required for apical constriction induction.
  • Cellular localization

    Cell junction > adherens junction. Cytoplasm > cytoskeleton. Colocalizes with F-actin in stress fibers and adherens junctions.
  • Information by UniProt
  • Database links

  • Alternative names

    • APXL3 antibody
    • hShrmL antibody
    • MSTP013 antibody
    • Protein Shroom3 antibody
    • Shrm antibody
    • Shrm3 antibody
    • SHRM3_HUMAN antibody
    • ShrmL antibody
    • Shroom family member 3 antibody
    • Shroom-related protein antibody
    • shroom3 antibody
    see all

Images

  • Immunohistochemical analysis of Paraffin-embedded Human rectum tissue labeling Shroom 3 with ab151009 at 1/50 dilution.

References

ab151009 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate buffer
Sample
Mouse Tissue sections (colon)
Specification
colon
Permeabilization
No
Fixative
Paraformaldehyde

Prof. F Van Roy

Verified customer

Submitted Sep 24 2013

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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