• Product name
  • Description
    Rabbit polyclonal to Shugoshin
  • Host species
  • Specificity
    There are 7 isoforms of Shugoshin with the following sizes: 64kDa, 58kDa, 34kDa, 32kD, 30kDa, 28kDa, and 24kDa. The immunogen used for this antibody is present in all isoforms. This antibody interacts with several proteins in the unsynchronized cell lines tested, with the most prominent bands in the range of 30-34kDa. The cell samples may need to be synchronized to detect the larger isoforms (see PLoS Biol. 2005 Mar;3(3):e86). These bands are competed away by addition of the immunizing peptide.
  • Tested applications
    Suitable for: WB, ICC/IF, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human Shugoshin.

    Read Abcam's proprietary immunogen policy (Peptide available as ab23438.)

  • Positive control
    • This antibody gave a positive signal in HeLa Whole Cell lysate.



Our Abpromise guarantee covers the use of ab21633 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 64 kDa (predicted molecular weight: 64 kDa).
ICC/IF 1/1000. PubMed: 18772885
IHC-P 1/350. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.


  • Function
    Plays a central role in chromosome cohesion during mitosis by preventing premature dissociation of cohesin complex from centromeres after prophase, when most of cohesin complex dissociates from chromosomes arms. May act by preventing phosphorylation of the STAG2 subunit of cohesin complex at the centromere, ensuring cohesin persistence at centromere until cohesin cleavage by ESPL1/separase at anaphase. Essential for proper chromosome segregation during mitosis and this function requires interaction with PPP2R1A. Its phosphorylated form is necessary for chromosome congression and for the proper attachment of spindle microtubule to the kinetochore. Necessary for kinetochore localization of PLK1 and CENPF. May play a role in the tension sensing mechanism of the spindle-assembly checkpoint by regulating PLK1 kinetochore affinity. Isoform 3 plays a role in maintaining centriole cohesion involved in controlling spindle pole integrity.
  • Tissue specificity
    Widely expressed. Highly expressed in testis. Expressed in lung, small intestine, breast, liver and placenta. Strongly overexpressed in 90% of breast cancers tested.
  • Sequence similarities
    Belongs to the shugoshin family.
  • Developmental stage
    Appears in prophase cells and remains present until metaphase. Strongly decreases at the onset of anaphase and completely disapears at telophase. Not present in interphase cells (at protein level).
  • Domain
    The KEN box and D-box 3 are required for its ubiquitination and degradation.
  • Post-translational
    Ubiquitinated and degraded during mitotic exit by APC/C-Cdh1.
    Phosphorylation by NEK2 is essential for chromosome congression in mitosis and for the proper attachment of spindle microtubule to the kinetochore. Phosphorylated by PLK1 and AUKRB.
  • Cellular localization
    Nucleus. Chromosome > centromere. Chromosome > centromere > kinetochore. Cytoplasm > cytoskeleton > spindle pole. Cytoplasm > cytoskeleton > centrosome. Localizes to the inner centromere throughout prophase until metaphase and disappears at anaphase. During prometaphase, it localizes to a single focus, while at metaphase, it localizes to 2 spots corresponding to the 2 centromeres. Centromeric localization requires the presence of BUB1 and the interaction with PPP2R1A. Localizes to the inner kinetochore from prophase to early metaphase. Co-localizes with NEK2 and SS18L1 at the kinetochore. Phosphorylation by AUKRB and the presence of BUB1 are required for localization to the kinetochore. Isoform 1 primarily localizes to kinetochores during G2 phase and mitotic prophase, metaphase, and anaphase and does not appear to be associated with kinetochores during late mitosis. Isoform 3 is found at the centrosome in interphase and at spindle poles in mitosis and its spindle pole localization is PLK1 dependent. Isoform 3 does not localize to kinetochores during any stages of the cell cycle.
  • Information by UniProt
  • Database links
  • Alternative names
    • hSgo 1 antibody
    • hSgo1 antibody
    • NY BR 85 antibody
    • Serologically defined breast cancer antigen NY BR 85 antibody
    • Serologically defined breast cancer antigen NY-BR-85 antibody
    • SGO 1 antibody
    • SGO antibody
    • SGO L1 antibody
    • SGO1 antibody
    • SGOL 1 antibody
    • SGOL1 antibody
    • SGOL1_HUMAN antibody
    • Shugoshin 1AB protein antibody
    • Shugoshin 1CD protein antibody
    • Shugoshin 1EFprotein antibody
    • Shugoshin 1GH protein antibody
    • Shugoshin 1KL protein antibody
    • Shugoshin like 1 (S. pombe) antibody
    • Shugoshin like 1 antibody
    • Shugoshin-like 1 antibody
    see all


  • Anti-Shugoshin antibody (ab21633) at 1 µg/ml + HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg


    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Performed under reducing conditions.

    Predicted band size: 64 kDa
    Observed band size: 64 kDa
    Additional bands at: 34 kDa, 37 kDa, 40 kDa. We are unsure as to the identity of these extra bands.

    Exposure time: 20 minutes
  • Image courtesy of Human Protein Atlas

    ab21633 staining Shugoshin in Human hippocampus. Paraffin embedded human hippocampus tissue was incubated with ab21633 (1/350 dilution) for 30 mins at room temperature. Antigen retrieval was performed by heat induction in citrate buffer pH 6.

    ab21633 was tested in a tissue microarray (TMA) containing a wide range of normal and cancer tissues as well as a cell microarray consisting of a range of commonly used, well characterised human cell lines. Further results for this antibody can be found at www.proteinatlas.org


This product has been referenced in:
  • Murakami-Tonami Y  et al. SGO1 is involved in the DNA damage response in MYCN-amplified neuroblastoma cells. Sci Rep 6:31615 (2016). Mouse . Read more (PubMed: 27539729) »
  • Lee MY  et al. Differential expression of centrosome regulators in Her2+ breast cancer cells versus non-tumorigenic MCF10A cells. Cell Div 9:3 (2014). Read more (PubMed: 25278993) »
See all 9 Publications for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Immunocytochemistry/ Immunofluorescence
Human Cell (HeLa)
Yes - 0.1% Trition
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 25°C

Ms. Chelsea Backer

Verified customer

Submitted Oct 24 2008

Abcam has not validated the combination of species/application used in this Abreview.
Human Cell (HeLa)

Dr. Kirk Mcmanus

Verified customer

Submitted Jun 07 2006


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