Overview

  • Product name

  • Description

    Rabbit polyclonal to Shugoshin
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Recombinant fragment corresponding to Human Shugoshin aa 260-439.
    Database link: Q5FBB7

  • Positive control

    • WB: MCF7 and HeLa whole cell lysates; Mouse brain tissue lysate. ICC/IF: HeLa cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab225948 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/5000. Detects a band of approximately 65 kDa (predicted molecular weight: 60,64 kDa).
ICC/IF 1/50 - 1/200.

Target

  • Function

    Plays a central role in chromosome cohesion during mitosis by preventing premature dissociation of cohesin complex from centromeres after prophase, when most of cohesin complex dissociates from chromosomes arms. May act by preventing phosphorylation of the STAG2 subunit of cohesin complex at the centromere, ensuring cohesin persistence at centromere until cohesin cleavage by ESPL1/separase at anaphase. Essential for proper chromosome segregation during mitosis and this function requires interaction with PPP2R1A. Its phosphorylated form is necessary for chromosome congression and for the proper attachment of spindle microtubule to the kinetochore. Necessary for kinetochore localization of PLK1 and CENPF. May play a role in the tension sensing mechanism of the spindle-assembly checkpoint by regulating PLK1 kinetochore affinity. Isoform 3 plays a role in maintaining centriole cohesion involved in controlling spindle pole integrity.
  • Tissue specificity

    Widely expressed. Highly expressed in testis. Expressed in lung, small intestine, breast, liver and placenta. Strongly overexpressed in 90% of breast cancers tested.
  • Sequence similarities

    Belongs to the shugoshin family.
  • Developmental stage

    Appears in prophase cells and remains present until metaphase. Strongly decreases at the onset of anaphase and completely disapears at telophase. Not present in interphase cells (at protein level).
  • Domain

    The KEN box and D-box 3 are required for its ubiquitination and degradation.
  • Post-translational
    modifications

    Ubiquitinated and degraded during mitotic exit by APC/C-Cdh1.
    Phosphorylation by NEK2 is essential for chromosome congression in mitosis and for the proper attachment of spindle microtubule to the kinetochore. Phosphorylated by PLK1 and AUKRB.
  • Cellular localization

    Nucleus. Chromosome > centromere. Chromosome > centromere > kinetochore. Cytoplasm > cytoskeleton > spindle pole. Cytoplasm > cytoskeleton > centrosome. Localizes to the inner centromere throughout prophase until metaphase and disappears at anaphase. During prometaphase, it localizes to a single focus, while at metaphase, it localizes to 2 spots corresponding to the 2 centromeres. Centromeric localization requires the presence of BUB1 and the interaction with PPP2R1A. Localizes to the inner kinetochore from prophase to early metaphase. Co-localizes with NEK2 and SS18L1 at the kinetochore. Phosphorylation by AUKRB and the presence of BUB1 are required for localization to the kinetochore. Isoform 1 primarily localizes to kinetochores during G2 phase and mitotic prophase, metaphase, and anaphase and does not appear to be associated with kinetochores during late mitosis. Isoform 3 is found at the centrosome in interphase and at spindle poles in mitosis and its spindle pole localization is PLK1 dependent. Isoform 3 does not localize to kinetochores during any stages of the cell cycle.
  • Information by UniProt
  • Database links

  • Alternative names

    • hSgo 1 antibody
    • hSgo1 antibody
    • NY BR 85 antibody
    • Serologically defined breast cancer antigen NY BR 85 antibody
    • Serologically defined breast cancer antigen NY-BR-85 antibody
    • SGO 1 antibody
    • SGO antibody
    • SGO L1 antibody
    • SGO1 antibody
    • SGOL 1 antibody
    • SGOL1 antibody
    • SGOL1_HUMAN antibody
    • Shugoshin 1AB protein antibody
    • Shugoshin 1CD protein antibody
    • Shugoshin 1EFprotein antibody
    • Shugoshin 1GH protein antibody
    • Shugoshin 1KL protein antibody
    • Shugoshin like 1 (S. pombe) antibody
    • Shugoshin like 1 antibody
    • Shugoshin-like 1 antibody
    see all

Images

  • All lanes : Anti-Shugoshin antibody (ab225948) at 1/500 dilution

    Lane 1 : MCF7 (human breast adenocarcinoma cell line) whole cell lysate
    Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
    Lane 3 : Mouse brain tissue lysate

    Secondary
    All lanes : Goat polyclonal to rabbit IgG at 1/50000 dilution

    Developed using the ECL technique.

    Predicted band size: 60,64 kDa
    Observed band size: 65 kDa
    why is the actual band size different from the predicted?

  • HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for Shugoshin (green) using ab225948 at 1/100 dilution in ICC/IF, followed by Alexa Fluor® 488-conjugated Goat Anti-Rabbit IgG (H+L).

References

ab225948 has not yet been referenced specifically in any publications.

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