Overview

  • Product name

  • Description

    Rabbit polyclonal to SI1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide within Human SI1 aa 398-427 (internal sequence) conjugated to keyhole limpet haemocyanin. The exact sequence is proprietary.
    Database link: Q9P2N6

  • Positive control

    • IHC-P: Human breast tissue. WB: HepG2, MDA-MB-453 and HEK-293 cell lysate.
  • General notes

     This product was previously labelled as KIAA1310

     

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab230550 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Predicted molecular weight: 96 kDa.
IHC-P 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Target

Images

  • All lanes : Anti-SI1 antibody (ab230550) at 1/1000 dilution

    Lane 1 : HepG2 (human liver hepatocellular carcinoma cell line) cell lysate
    Lane 2 : MDA-MB-453 cell lysate
    Lane 3 : HEK-293 (human epithelial cell line from embryonic kidney) cell lysate

    Lysates/proteins at 35 µg per lane.

    Developed using the ECL technique.

    Predicted band size: 96 kDa

  • Formalin-fixed, paraffin-embedded human breast tissue stained for SI1 with ab230550 at 1/100 dilution in immunohistochemical analysis.

References

ab230550 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab230550.
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