Anti-SIA8C antibody - C-terminal (ab174958)

Overview

  • Product name
    Anti-SIA8C antibody - C-terminal
    See all SIA8C primary antibodies
  • Description
    Rabbit polyclonal to SIA8C - C-terminal
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, Flow Cytmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic peptide within Human SIA8C aa 319-348 (C terminal) conjugated to Keyhole Limpet Haemocyanin (KLH). The exact sequence is proprietary.
    Database link: O43173

  • Positive control
    • HL60 cells

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.09% Sodium azide
    Constituent: 99% PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab174958 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/100 - 1/500. Predicted molecular weight: 44 kDa.
Flow Cyt 1/10 - 1/50.

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

 

Target

  • Function
    May transfer sialic acid through alpha-2,8-linkages to alpha-2,3-linked and alpha-2,8-linked sialic acid of N-linked oligosaccharides of glycoproteins and glycolipids. It can form polysialic acid in vitro directly on alpha-2,3-, alpha-2,6-, or alpha-2,8-linked sialic acid.
  • Tissue specificity
    Expressed in fetal and adult brain and fetal liver.
  • Pathway
    Protein modification; protein glycosylation.
  • Sequence similarities
    Belongs to the glycosyltransferase 29 family.
  • Cellular localization
    Golgi apparatus membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • 3-Gal-beta-1 antibody
    • 4-GlcNAc-R:alpha 2 antibody
    • 8-sialyltransferase 3 antibody
    • 8-sialyltransferase 8C antibody
    • 8-sialyltransferase antibody
    • 8-sialyltransferase III antibody
    • Alpha-2 antibody
    • Sia-alpha-2 antibody
    • SIA8C_HUMAN antibody
    • Sialyltransferase 8C antibody
    • Sialyltransferase St8Sia III antibody
    • SIAT8-C antibody
    • ST8 alpha-N-acetyl-neuraminide alpha-2 antibody
    • St8sia3 antibody
    • ST8SiaIII antibody
    see all

Images

  • Anti-SIA8C antibody - C-terminal (ab174958) at 1/100 dilution + HL60 cell lysate at 15 µg

    Predicted band size: 44 kDa

  • Flow cytometric analysis of HL60 cells (right histogram) labeling SIA8C using ab174958 at 1/10 dilution, compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

References

ab174958 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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