Recombinant
RabMAb

Anti-SIAH Interacting Protein antibody [EPR12374] (ab171972)

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Overview

  • Product name
    Anti-SIAH Interacting Protein antibody [EPR12374]
    See all SIAH Interacting Protein primary antibodies
  • Description
    Rabbit monoclonal [EPR12374] to SIAH Interacting Protein
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IF, IP, Flow Cytmore details
  • Species reactivity
    Reacts with: Human
    Does not react with: Mouse, Rat
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human SIAH Interacting Protein aa 200 to the C-terminus (Cysteine residue). The exact sequence is proprietary.
    Database link: Q9HB71

  • Positive control
    • Human kidney, gastric carcinoma and gliocytoma tissues; MCF7, HeLa, 293T and PC3 cell lysates; MCF7 and 293T cells.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
  • Purity
    Tissue culture supernatant
  • Clonality
    Monoclonal
  • Clone number
    EPR12374
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab171972 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Predicted molecular weight: 26 kDa.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF 1/100 - 1/250.
IP 1/10 - 1/100.
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

Target

  • Function
    May be involved in calcium-dependent ubiquitination and subsequent proteasomal degradation of target proteins. Probably serves as a molecular bridge in ubiquitin E3 complexes. Participates in the ubiquitin-mediated degradation of beta-catenin (CTNNB1).
  • Sequence similarities
    Contains 1 CS domain.
    Contains 1 SGS domain.
  • Post-translational
    modifications
    Phosphorylated on serine residues. Phosphorylated upon induction by RA or at high calcium concentrations.
  • Cellular localization
    Nucleus. Cytoplasm. Cytoplasmic at low calcium concentrations. In neuroblastoma cells, after a retinoic acid (RA) induction and calcium increase, it localizes in both the nucleus and cytoplasm. The nuclear fraction may be phosphorylated.
  • Information by UniProt
  • Database links
    • Alternative names
      • CacyBP antibody
      • Calcyclin binding protein antibody
      • Calcyclin-binding protein antibody
      • CYBP_HUMAN antibody
      • GIG 5 antibody
      • GIG5 antibody
      • Growth inhibiting gene 5 antibody
      • Growth inhibiting gene 5 protein antibody
      • hCacyBP antibody
      • MGC87971 antibody
      • PNAS 107 antibody
      • PNAS107 antibody
      • RP1 102G20.6 antibody
      • S100A6 binding protein antibody
      • S100A6-binding protein antibody
      • S100A6BP antibody
      • Siah-interacting protein antibody
      • SIP antibody
      see all

    Images

    • Western blot - Anti-SIAH Interacting Protein antibody [EPR12374] (ab171972)
      Western blot - Anti-SIAH Interacting Protein antibody [EPR12374] (ab171972)
      All lanes : Anti-SIAH Interacting Protein antibody [EPR12374] (ab171972) at 1/10000 dilution

      Lane 1 : MCF7 cell lysate
      Lane 2 : HeLa cell lysate
      Lane 3 : 293T cell lysate
      Lane 4 : PC3 cell lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 26 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SIAH Interacting Protein antibody [EPR12374] (ab171972)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SIAH Interacting Protein antibody [EPR12374] (ab171972)

      Immunohistochemical analysis of paraffin-embedded Human Kidney tissue labeling SIAH Interacting Protein with ab171972 at 1/100 dilution.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SIAH Interacting Protein antibody [EPR12374] (ab171972)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SIAH Interacting Protein antibody [EPR12374] (ab171972)

      Immunohistochemical analysis of paraffin-embedded Human gliocytoma tissue labeling SIAH Interacting Protein with ab171972 at 1/100 dilution.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SIAH Interacting Protein antibody [EPR12374] (ab171972)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SIAH Interacting Protein antibody [EPR12374] (ab171972)

      Immunohistochemical analysis of paraffin-embedded Human gastric carcinoma tissue labeling SIAH Interacting Protein with ab171972 at 1/100 dilution.

    • Immunocytochemistry/ Immunofluorescence - Anti-SIAH Interacting Protein antibody [EPR12374] (ab171972)
      Immunocytochemistry/ Immunofluorescence - Anti-SIAH Interacting Protein antibody [EPR12374] (ab171972)

      Immunofluorescent analysis of MCF7 cells labeling SIAH Interacting Protein with ab171972 at 1/100 dilution.  DAPI nuclear staining (blue).

    • Immunoprecipitation - Anti-SIAH Interacting Protein antibody [EPR12374] (ab171972)
      Immunoprecipitation - Anti-SIAH Interacting Protein antibody [EPR12374] (ab171972)

      Western blot analysis on immunoprecipitation pellet from 293T cell lysate (lane 1) labeling SIAH Interacting Protein with ab171972 at 1/10 dilution or 1X PBS (lane 2) negative control.  HRP-conjugated anti-Rabbit IgG preferentially detecting the non-reduced form of Rabbit IgG.

    • Flow Cytometry - Anti-SIAH Interacting Protein [EPR12374] antibody (ab171972)
      Flow Cytometry - Anti-SIAH Interacting Protein [EPR12374] antibody (ab171972)

      Flow cytometric analysis of permeabilized 293T cells labeling SIAH Interacting Protein with ab171972 at 1/10 dilution (red) or  Rabbit IgG (negative) (green).

    References

    ab171972 has not yet been referenced specifically in any publications.

    Publishing research using ab171972? Please let us know so that we can cite the reference in this datasheet.

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