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Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human SIAH Interacting Protein aa 200 to the C-terminus (Cysteine residue). The exact sequence is proprietary.
Database link: Q9HB71
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
This product is a recombinant rabbit monoclonal antibody.
Our Abpromise guarantee covers the use of ab171972 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/10000 - 1/50000. Predicted molecular weight: 26 kDa.|
|IHC-P||1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|ICC/IF||1/100 - 1/250.|
|IP||1/10 - 1/100.|
|Flow Cyt||1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
Immunohistochemical analysis of paraffin-embedded Human Kidney tissue labeling SIAH Interacting Protein with ab171972 at 1/100 dilution.
Immunohistochemical analysis of paraffin-embedded Human gliocytoma tissue labeling SIAH Interacting Protein with ab171972 at 1/100 dilution.
Immunohistochemical analysis of paraffin-embedded Human gastric carcinoma tissue labeling SIAH Interacting Protein with ab171972 at 1/100 dilution.
Immunofluorescent analysis of MCF7 cells labeling SIAH Interacting Protein with ab171972 at 1/100 dilution. DAPI nuclear staining (blue).
Western blot analysis on immunoprecipitation pellet from 293T cell lysate (lane 1) labeling SIAH Interacting Protein with ab171972 at 1/10 dilution or 1X PBS (lane 2) negative control. HRP-conjugated anti-Rabbit IgG preferentially detecting the non-reduced form of Rabbit IgG.
Flow cytometric analysis of permeabilized 293T cells labeling SIAH Interacting Protein with ab171972 at 1/10 dilution (red) or Rabbit IgG (negative) (green).
ab171972 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"