Anti-SIAH Interacting Protein antibody [EPR12374] (ab171972)
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Overview
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Product nameAnti-SIAH Interacting Protein antibody [EPR12374]
See all SIAH Interacting Protein primary antibodies -
DescriptionRabbit monoclonal [EPR12374] to SIAH Interacting Protein
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Host speciesRabbit
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Tested applicationsSuitable for: WB, IHC-P, ICC/IF, IP, Flow Cytmore details
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Species reactivityReacts with: Human
Does not react with: Mouse, Rat -
Immunogen
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human SIAH Interacting Protein aa 200 to the C-terminus (Cysteine residue). The exact sequence is proprietary.
Database link: Q9HB71 -
Positive control
- Human kidney, gastric carcinoma and gliocytoma tissues; MCF7, HeLa, 293T and PC3 cell lysates; MCF7 and 293T cells.
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General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
This product is a recombinant rabbit monoclonal antibody.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
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Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant -
PurityTissue culture supernatant
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ClonalityMonoclonal
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Clone numberEPR12374
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IsotypeIgG
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Research areas
Associated products
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Isotype control
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab171972 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/10000 - 1/50000. Predicted molecular weight: 26 kDa. | |
| IHC-P | 1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. | |
| ICC/IF | 1/100 - 1/250. | |
| IP | 1/10 - 1/100. | |
| Flow Cyt | 1/10 - 1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
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Target
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FunctionMay be involved in calcium-dependent ubiquitination and subsequent proteasomal degradation of target proteins. Probably serves as a molecular bridge in ubiquitin E3 complexes. Participates in the ubiquitin-mediated degradation of beta-catenin (CTNNB1).
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Sequence similaritiesContains 1 CS domain.
Contains 1 SGS domain. -
Post-translational
modificationsPhosphorylated on serine residues. Phosphorylated upon induction by RA or at high calcium concentrations. -
Cellular localizationNucleus. Cytoplasm. Cytoplasmic at low calcium concentrations. In neuroblastoma cells, after a retinoic acid (RA) induction and calcium increase, it localizes in both the nucleus and cytoplasm. The nuclear fraction may be phosphorylated.
- Information by UniProt
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Database links
- Entrez Gene: 27101 Human
- Omim: 606186 Human
- SwissProt: Q9HB71 Human
- Unigene: 508524 Human
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Alternative names
- CacyBP antibody
- Calcyclin binding protein antibody
- Calcyclin-binding protein antibody
see all
Images
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![Western blot - Anti-SIAH Interacting Protein antibody [EPR12374] (ab171972)](https://www.abcam.com/ps/products/171/ab171972/Images/ab171972-194476-8.jpg)
Western blot - Anti-SIAH Interacting Protein antibody [EPR12374] (ab171972)All lanes : Anti-SIAH Interacting Protein antibody [EPR12374] (ab171972) at 1/10000 dilution
Lane 1 : MCF7 cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : 293T cell lysate
Lane 4 : PC3 cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 26 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SIAH Interacting Protein antibody [EPR12374] (ab171972)](https://www.abcam.com/ps/products/171/ab171972/Images/ab171972-194474-6.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SIAH Interacting Protein antibody [EPR12374] (ab171972)Immunohistochemical analysis of paraffin-embedded Human Kidney tissue labeling SIAH Interacting Protein with ab171972 at 1/100 dilution.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SIAH Interacting Protein antibody [EPR12374] (ab171972)](https://www.abcam.com/ps/products/171/ab171972/Images/ab171972-194473-5.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SIAH Interacting Protein antibody [EPR12374] (ab171972)Immunohistochemical analysis of paraffin-embedded Human gliocytoma tissue labeling SIAH Interacting Protein with ab171972 at 1/100 dilution.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SIAH Interacting Protein antibody [EPR12374] (ab171972)](https://www.abcam.com/ps/products/171/ab171972/Images/ab171972-194472-4.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SIAH Interacting Protein antibody [EPR12374] (ab171972)Immunohistochemical analysis of paraffin-embedded Human gastric carcinoma tissue labeling SIAH Interacting Protein with ab171972 at 1/100 dilution.
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![Immunocytochemistry/ Immunofluorescence - Anti-SIAH Interacting Protein antibody [EPR12374] (ab171972)](https://www.abcam.com/ps/products/171/ab171972/Images/ab171972-194471-3.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-SIAH Interacting Protein antibody [EPR12374] (ab171972)Immunofluorescent analysis of MCF7 cells labeling SIAH Interacting Protein with ab171972 at 1/100 dilution. DAPI nuclear staining (blue).
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![Immunoprecipitation - Anti-SIAH Interacting Protein antibody [EPR12374] (ab171972)](https://www.abcam.com/ps/products/171/ab171972/Images/ab171972-194475-7.jpg)
Immunoprecipitation - Anti-SIAH Interacting Protein antibody [EPR12374] (ab171972)Western blot analysis on immunoprecipitation pellet from 293T cell lysate (lane 1) labeling SIAH Interacting Protein with ab171972 at 1/10 dilution or 1X PBS (lane 2) negative control. HRP-conjugated anti-Rabbit IgG preferentially detecting the non-reduced form of Rabbit IgG.
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![Flow Cytometry - Anti-SIAH Interacting Protein [EPR12374] antibody (ab171972)](https://www.abcam.com/ps/products/171/ab171972/Images/ab171972-194469-ab1719721944692.jpg)
Flow Cytometry - Anti-SIAH Interacting Protein [EPR12374] antibody (ab171972)Flow cytometric analysis of permeabilized 293T cells labeling SIAH Interacting Protein with ab171972 at 1/10 dilution (red) or Rabbit IgG (negative) (green).
Datasheets and documents
Protocols
References
ab171972 has not yet been referenced specifically in any publications.
