Product nameAnti-SIAH Interacting Protein antibody [EPR12374]
See all SIAH Interacting Protein primary antibodies
DescriptionRabbit monoclonal [EPR12374] to SIAH Interacting Protein
Tested applicationsSuitable for: WB, IHC-P, ICC/IF, IP, Flow Cytmore details
Species reactivityReacts with: Human
Does not react with: Mouse, Rat
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human SIAH Interacting Protein aa 200 to the C-terminus (Cysteine residue). The exact sequence is proprietary.
Database link: Q9HB71
- Human kidney, gastric carcinoma and gliocytoma tissues; MCF7, HeLa, 293T and PC3 cell lysates; MCF7 and 293T cells.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
This product is a recombinant rabbit monoclonal antibody.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab171972 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/10000 - 1/50000. Predicted molecular weight: 26 kDa.|
|IHC-P||1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|ICC/IF||1/100 - 1/250.|
|IP||1/10 - 1/100.|
|Flow Cyt||1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
FunctionMay be involved in calcium-dependent ubiquitination and subsequent proteasomal degradation of target proteins. Probably serves as a molecular bridge in ubiquitin E3 complexes. Participates in the ubiquitin-mediated degradation of beta-catenin (CTNNB1).
Sequence similaritiesContains 1 CS domain.
Contains 1 SGS domain.
modificationsPhosphorylated on serine residues. Phosphorylated upon induction by RA or at high calcium concentrations.
Cellular localizationNucleus. Cytoplasm. Cytoplasmic at low calcium concentrations. In neuroblastoma cells, after a retinoic acid (RA) induction and calcium increase, it localizes in both the nucleus and cytoplasm. The nuclear fraction may be phosphorylated.
- Information by UniProt
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All lanes : Anti-SIAH Interacting Protein antibody [EPR12374] (ab171972) at 1/10000 dilution
Lane 1 : MCF7 cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : 293T cell lysate
Lane 4 : PC3 cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 26 kDa
Immunohistochemical analysis of paraffin-embedded Human Kidney tissue labeling SIAH Interacting Protein with ab171972 at 1/100 dilution.
Immunohistochemical analysis of paraffin-embedded Human gliocytoma tissue labeling SIAH Interacting Protein with ab171972 at 1/100 dilution.
Immunohistochemical analysis of paraffin-embedded Human gastric carcinoma tissue labeling SIAH Interacting Protein with ab171972 at 1/100 dilution.
Immunofluorescent analysis of MCF7 cells labeling SIAH Interacting Protein with ab171972 at 1/100 dilution. DAPI nuclear staining (blue).
Western blot analysis on immunoprecipitation pellet from 293T cell lysate (lane 1) labeling SIAH Interacting Protein with ab171972 at 1/10 dilution or 1X PBS (lane 2) negative control. HRP-conjugated anti-Rabbit IgG preferentially detecting the non-reduced form of Rabbit IgG.
Flow cytometric analysis of permeabilized 293T cells labeling SIAH Interacting Protein with ab171972 at 1/10 dilution (red) or Rabbit IgG (negative) (green).
ab171972 has not yet been referenced specifically in any publications.