Anti-SIAH1 antibody (ab2237)
Key features and details
- Goat polyclonal to SIAH1
- Suitable for: WB, ICC/IF
- Reacts with: Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-SIAH1 antibody
See all SIAH1 primary antibodies -
Description
Goat polyclonal to SIAH1 -
Host species
Goat -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Rat, Human
Predicted to work with: Mouse, Cow, Dog -
Immunogen
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Positive control
- WB: Rat liver lysate, human liver lysate. ICC/IF- U2OS cells and HepG2 cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.05% Tris, 0.5% BSA -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab2237 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (1) |
Use a concentration of 1 - 3 µg/ml. Predicted molecular weight: 31 kDa.
1 hour primary incubation at room temperature is recommended for this product. |
ICC/IF |
Use a concentration of 10 µg/ml.
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Notes |
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WB
Use a concentration of 1 - 3 µg/ml. Predicted molecular weight: 31 kDa. 1 hour primary incubation at room temperature is recommended for this product. |
ICC/IF
Use a concentration of 10 µg/ml. |
Target
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Function
E3 ubiquitin-protein ligase that mediates ubiquitination and subsequent proteasomal degradation of target proteins. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Mediates E3 ubiquitin ligase activity either through direct binding to substrates or by functioning as the essential RING domain subunit of larger E3 complexes. Triggers the ubiquitin-mediated degradation of many substrates, including proteins involved in transcription regulation (MYB, POU2AF1, PML and RBBP8), a cell surface receptor (DCC), the cell-surface receptor-type tyrosine kinase FLT3, the cytoplasmic signal transduction molecules (KLF10/TIEG1 and NUMB), an antiapoptotic protein (BAG1), a microtubule motor protein (KIF22), a protein involved in synaptic vesicle function in neurons (SYP), a structural protein (CTNNB1) and SNCAIP. Confers constitutive instability to HIPK2 through proteasomal degradation. It is thereby involved in many cellular processes such as apoptosis, tumor suppression, cell cycle, axon guidance, transcription regulation, spermatogenesis and TNF-alpha signaling. Has some overlapping function with SIAH2. Induces apoptosis in cooperation with PEG3. Upon nitric oxid (NO) generation that follows apoptotic stimulation, interacts with S-nitrosylated GAPDH, mediating the translocation of GAPDH to the nucleus. GAPDH acts as a stabilizer of SIAH1, facilitating the degradation of nuclear proteins. -
Tissue specificity
Widely expressed at a low level. Down-regulated in advanced hepatocellular carcinomas. -
Pathway
Protein modification; protein ubiquitination. -
Sequence similarities
Belongs to the SINA (Seven in absentia) family.
Contains 1 RING-type zinc finger.
Contains 1 SIAH-type zinc finger. -
Domain
The RING-type zinc finger domain is essential for ubiquitin ligase activity.
The SBD domain (substrate-binding domain) mediates the homodimerization and the interaction with substrate proteins. It is related to the TRAF family. -
Post-translational
modificationsPhosphorylated on Ser-19 by ATM and ATR. This phosphorylation disrupts SIAH1 interaction with HIPK2, and subsequent proteasomal degradation of HIPK2. -
Cellular localization
Cytoplasm. Nucleus. Predominantly cytoplasmic. Partially nuclear. - Information by UniProt
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Database links
- Entrez Gene: 534655 Cow
- Entrez Gene: 6477 Human
- Entrez Gene: 20437 Mouse
- Entrez Gene: 140941 Rat
- Omim: 602212 Human
- SwissProt: Q8IUQ4 Human
- SwissProt: P61092 Mouse
- SwissProt: Q920M9 Rat
see all -
Alternative names
- E3 ubiquitin-protein ligase SIAH1 antibody
- FLJ08065 antibody
- hSIAH1 antibody
see all
Images
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Anti-SIAH1 antibody (ab2237) at 1 mg/ml + Human liver lysate at 35 µg
Predicted band size: 31 kDa
Observed band size: 37 kDa why is the actual band size different from the predicted?Primary incubation 1 hour at room temperature. Detected by chemiluminescence. RIPA buffer was used.
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Anti-SIAH1 antibody (ab2237) at 1 µg/ml + Rat liver lysate at 35 µg
Predicted band size: 31 kDa
Observed band size: 30, 37 kDa why is the actual band size different from the predicted?Primary incubation 1 hour at room temperature. Detected by chemiluminescence. RIPA buffer was used.
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Immunofluorescence analysis of paraformaldehyde fixed, 0.15% Triton permeabilized HepG2 (human liver hepatocellular carcinoma cell) cells labelling SIAH1 with primary anti-SIAH1 antibody (ab2237) at 10ug/ml for 1 hour, followed by Alexa Fluor 488 secondary antibody at 2ug/ml. Image showing nuclear and cytoplasmic staining. The nuclear counterstain is DAPI (blue).
Negative control: Unimmunized goat IgG at 10ug/ml followed by Alexa Fluor 488 secondary antibody.
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Immunofluorescence analysis of paraformaldehyde fixed, 0.15% Triton permeabilized U2OS (human bone osteosarcoma epithelial cell) cells labelling SIAH1 with primary anti-SIAH1 antibody (ab2237) at 10ug/ml for 1 hour, followed by Alexa Fluor 488 secondary antibody at 2ug/ml. Image showing nuclear and cytoplasmic staining. The nuclear counterstain is DAPI (blue).
Negative control: Unimmunized goat IgG at 10ug/ml followed by Alexa Fluor 488 secondary antibody.
Datasheets and documents
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Datasheet download
References (33)
ab2237 has been referenced in 33 publications.
- Lin L et al. Reactive oxygen species-induced SIAH1 promotes granulosa cells' senescence in premature ovarian failure. J Cell Mol Med 26:2417-2427 (2022). PubMed: 35261172
- Gao W et al. SIAH1 reverses chemoresistance in epithelial ovarian cancer via ubiquitination of YBX-1. Oncogenesis 11:13 (2022). PubMed: 35273154
- Schmitz ML et al. SIAH ubiquitin E3 ligases as modulators of inflammatory gene expression. Heliyon 8:e09029 (2022). PubMed: 35284677
- Xia L et al. m6A-induced repression of SIAH1 facilitates alternative splicing of androgen receptor variant 7 by regulating CPSF1. Mol Ther Nucleic Acids 28:219-230 (2022). PubMed: 35402071
- Huang F et al. P-TEFb is degraded by Siah1/2 in quiescent cells. Nucleic Acids Res 50:5000-5013 (2022). PubMed: 35524561