Product nameAnti-SIAH1 antibody
See all SIAH1 primary antibodies
DescriptionRabbit polyclonal to SIAH1
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Chicken, Cow, Dog, Drosophila melanogaster, Zebrafish
- Placenta lysate
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 2% Sucrose, PBS
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab49177 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1.25 µg/ml. Detects a band of approximately 34 kDa (predicted molecular weight: 31 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.|
FunctionE3 ubiquitin-protein ligase that mediates ubiquitination and subsequent proteasomal degradation of target proteins. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Mediates E3 ubiquitin ligase activity either through direct binding to substrates or by functioning as the essential RING domain subunit of larger E3 complexes. Triggers the ubiquitin-mediated degradation of many substrates, including proteins involved in transcription regulation (MYB, POU2AF1, PML and RBBP8), a cell surface receptor (DCC), the cell-surface receptor-type tyrosine kinase FLT3, the cytoplasmic signal transduction molecules (KLF10/TIEG1 and NUMB), an antiapoptotic protein (BAG1), a microtubule motor protein (KIF22), a protein involved in synaptic vesicle function in neurons (SYP), a structural protein (CTNNB1) and SNCAIP. Confers constitutive instability to HIPK2 through proteasomal degradation. It is thereby involved in many cellular processes such as apoptosis, tumor suppression, cell cycle, axon guidance, transcription regulation, spermatogenesis and TNF-alpha signaling. Has some overlapping function with SIAH2. Induces apoptosis in cooperation with PEG3. Upon nitric oxid (NO) generation that follows apoptotic stimulation, interacts with S-nitrosylated GAPDH, mediating the translocation of GAPDH to the nucleus. GAPDH acts as a stabilizer of SIAH1, facilitating the degradation of nuclear proteins.
Tissue specificityWidely expressed at a low level. Down-regulated in advanced hepatocellular carcinomas.
PathwayProtein modification; protein ubiquitination.
Sequence similaritiesBelongs to the SINA (Seven in absentia) family.
Contains 1 RING-type zinc finger.
Contains 1 SIAH-type zinc finger.
DomainThe RING-type zinc finger domain is essential for ubiquitin ligase activity.
The SBD domain (substrate-binding domain) mediates the homodimerization and the interaction with substrate proteins. It is related to the TRAF family.
modificationsPhosphorylated on Ser-19 by ATM and ATR. This phosphorylation disrupts SIAH1 interaction with HIPK2, and subsequent proteasomal degradation of HIPK2.
Cellular localizationCytoplasm. Nucleus. Predominantly cytoplasmic. Partially nuclear.
- Information by UniProt
- E3 ubiquitin-protein ligase SIAH1 antibody
- FLJ08065 antibody
- hSIAH1 antibody
Anti-SIAH1 antibody (ab49177) at 1.25 µg/ml + Human placenta lysate at 10 µg with 5% skimmed milk in PBS
HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Developed using the ECL technique.
Predicted band size: 31 kDa
Observed band size: 34 kDa why is the actual band size different from the predicted?
Gel concentration: 12%
This product has been referenced in:
- Das L et al. Membrane-bound ß-catenin degradation is enhanced by ETS2-mediated Siah1 induction in Helicobacter pylori-infected gastric cancer cells. Oncogenesis 6:e327 (2017). FM ; Human . Read more (PubMed: 28481365) »
- Delehouzé C et al. CDK/CK1 inhibitors roscovitine and CR8 downregulate amplified MYCN in neuroblastoma cells. Oncogene N/A:N/A (2013). Human . Read more (PubMed: 24317512) »