Key features and details
- Rabbit polyclonal to Siah2 - C-terminal
- Suitable for: ICC/IF, IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-Siah2 antibody - C-terminal
See all Siah2 primary antibodies
DescriptionRabbit polyclonal to Siah2 - C-terminal
Tested applicationsSuitable for: ICC/IF, IHC-P, WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Zebrafish
Synthetic peptide corresponding to Human Siah2 (C terminal) conjugated to keyhole limpet haemocyanin.
Database link: O43255
- IHC-P: Human colon tissue. ICC/IF: MCF7 cells. WB: MCF7 whole cell lysate.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.30
Preservative: 0.01% Sodium azide
Constituents: PBS, 30% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab230523 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||1/100 - 1/500.|
|WB||1/500 - 1/1000. Predicted molecular weight: 34 kDa.|
FunctionE3 ubiquitin-protein ligase that mediates ubiquitination and subsequent proteasomal degradation of target proteins. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Mediates E3 ubiquitin ligase activity either through direct binding to substrates or by functioning as the essential RING domain subunit of larger E3 complexes. Triggers the ubiquitin-mediated degradation of many substrates, including proteins involved in transcription regulation (POU2AF1, PML, NCOR1), a cell surface receptor (DCC), an antiapoptotic protein (BAG1), and a protein involved in synaptic vesicle function in neurons (SYP). It is thereby involved in apoptosis, tumor suppression, cell cycle, transcription and signaling processes. Has some overlapping function with SIAH1. Triggers the ubiquitin-mediated degradation of TRAF2, whereas SIAH1 can not. Promotes monoubiquitination of SNCA.
Tissue specificityWidely expressed at low level.
PathwayProtein modification; protein ubiquitination.
Sequence similaritiesBelongs to the SINA (Seven in absentia) family.
Contains 1 RING-type zinc finger.
Contains 1 SIAH-type zinc finger.
DomainThe RING-type zinc finger domain is essential for ubiquitin ligase activity.
The SBD domain (substrate-binding domain) mediates the homodimerization and the interaction with substrate proteins. It is related to the TRAF family.
Cellular localizationCytoplasm. Nucleus. Predominantly cytoplasmic (Probable). Partially nuclear.
- Information by UniProt
- E3 ubiquitin-protein ligase Siah2 antibody
- hSiah2 antibody
- Seven in absentia homolog 2 antibody
Anti-Siah2 antibody - C-terminal (ab230523) at 1/500 dilution + MCF7 (Human breast adenocarcinoma cell line) whole cell lysate
Developed using the ECL technique.
Predicted band size: 34 kDa
Formalin-fixed, paraffin-embedded human colon tissue stained for Siah2 with ab230523 at a 1/100 dilution in immunohistochemical analysis.
MCF7 (Human breast adenocarcinoma cell line) cells stained for Siah2 using ab230523 at a dilution of 1/100 in ICC/IF.
Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with ab230523 in 3% BSA-PBS and incubated overnight at 4°C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight® 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab230523 has not yet been referenced specifically in any publications.