Overview

  • Product name

    Anti-Siah2 antibody - C-terminal
    See all Siah2 primary antibodies
  • Description

    Rabbit polyclonal to Siah2 - C-terminal
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, IHC-P, WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Zebrafish
  • Immunogen

    Synthetic peptide corresponding to Human Siah2 (C terminal) conjugated to keyhole limpet haemocyanin.
    Database link: O43255

  • Positive control

    • IHC-P: Human colon tissue. ICC/IF: MCF7 cells. WB: MCF7 whole cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab230523 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/100 - 1/500.
IHC-P 1/100.
WB 1/500 - 1/1000. Predicted molecular weight: 34 kDa.

Target

  • Function

    E3 ubiquitin-protein ligase that mediates ubiquitination and subsequent proteasomal degradation of target proteins. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Mediates E3 ubiquitin ligase activity either through direct binding to substrates or by functioning as the essential RING domain subunit of larger E3 complexes. Triggers the ubiquitin-mediated degradation of many substrates, including proteins involved in transcription regulation (POU2AF1, PML, NCOR1), a cell surface receptor (DCC), an antiapoptotic protein (BAG1), and a protein involved in synaptic vesicle function in neurons (SYP). It is thereby involved in apoptosis, tumor suppression, cell cycle, transcription and signaling processes. Has some overlapping function with SIAH1. Triggers the ubiquitin-mediated degradation of TRAF2, whereas SIAH1 can not. Promotes monoubiquitination of SNCA.
  • Tissue specificity

    Widely expressed at low level.
  • Pathway

    Protein modification; protein ubiquitination.
  • Sequence similarities

    Belongs to the SINA (Seven in absentia) family.
    Contains 1 RING-type zinc finger.
    Contains 1 SIAH-type zinc finger.
  • Domain

    The RING-type zinc finger domain is essential for ubiquitin ligase activity.
    The SBD domain (substrate-binding domain) mediates the homodimerization and the interaction with substrate proteins. It is related to the TRAF family.
  • Cellular localization

    Cytoplasm. Nucleus. Predominantly cytoplasmic (Probable). Partially nuclear.
  • Information by UniProt
  • Database links

  • Alternative names

    • E3 ubiquitin-protein ligase Siah2 antibody
    • hSiah2 antibody
    • Seven in absentia homolog 2 antibody
    • Siah E3 ubiquitin protein ligase 2 antibody
    • Siah-2 antibody
    • siah2 antibody
    • SIAH2_HUMAN antibody
    • Ubiquitin Ligase Siah2 antibody
    see all

Images

  • Anti-Siah2 antibody - C-terminal (ab230523) at 1/500 dilution + MCF7 (Human breast adenocarcinoma cell line) whole cell lysate

    Developed using the ECL technique.

    Predicted band size: 34 kDa

  • Formalin-fixed, paraffin-embedded human colon tissue stained for Siah2 with ab230523 at a 1/100 dilution in immunohistochemical analysis.

  • MCF7 (Human breast adenocarcinoma cell line) cells stained for Siah2 using ab230523 at a dilution of 1/100 in ICC/IF.

    Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with ab230523 in 3% BSA-PBS and incubated overnight at 4°C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight® 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

References

ab230523 has not yet been referenced specifically in any publications.

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