Overview

  • Product name
    Anti-Sialoadhesin antibody [HSn 7D2]
    See all Sialoadhesin primary antibodies
  • Description
    Mouse monoclonal [HSn 7D2] to Sialoadhesin
  • Host species
    Mouse
  • Tested applications
    Suitable for: ICC/IF, WB, RIA, Flow Cyt, ELISA, IHC-P, IHC-Frmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length native protein (purified) corresponding to Human Sialoadhesin. Full length hSn(d1-4)Fc protein.

  • Epitope
    Within domains 1-4.
  • Positive control
    • WB: Human spleen and human lymph node whole tissue lysate. IHC-Fr: Immunohistochemistry (Frozen sections) d) Frozen Human liver normal tissue.
  • General notes

    This antibody clone is manufactured by Abcam. If you require a different buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.02% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • Purity
    Protein G purified
  • Clonality
    Monoclonal
  • Clone number
    HSn 7D2
  • Isotype
    IgG1
  • Light chain type
    kappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab18619 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration. PubMed: 20696778
WB Use a concentration of 1 µg/ml.
RIA Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent concentration.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

ELISA Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration.
IHC-Fr Use a concentration of 5 µg/ml.

Target

  • Function
    Acts as an endocytic receptor mediating clathrin dependent endocytosis. Macrophage-restricted adhesion molecule that mediates sialic-acid dependent binding to lymphocytes, including granulocytes, monocytes, natural killer cells, B-cells and CD8 T-cells. Preferentially binds to alpha-2,3-linked sialic acid (By similarity). Binds to SPN/CD43 on T-cells (By similarity). May play a role in hemopoiesis.
  • Tissue specificity
    Expressed by macrophages in various tissues. High levels are found in spleen, lymph node, perivascular macrophages in brain and lower levels in bone marrow, liver Kupffer cells and lamina propria of colon and lung. Also expressed by inflammatory macrophages in rheumatoid arthritis.
  • Sequence similarities
    Belongs to the immunoglobulin superfamily. SIGLEC (sialic acid binding Ig-like lectin) family.
    Contains 16 Ig-like C2-type (immunoglobulin-like) domains.
    Contains 1 Ig-like V-type (immunoglobulin-like) domain.
  • Cellular localization
    Secreted and Cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • CD169 antibody
    • CD169 antigen antibody
    • dJ1009E24.1 antibody
    • DKFZp667F058 antibody
    • FLJ00051 antibody
    • FLJ00055 antibody
    • FLJ00073 antibody
    • FLJ00411 antibody
    • FLJ32150 antibody
    • Sialic acid binding Ig like lectin 1 antibody
    • sialic acid binding Ig-like lectin 1, sialoadhesin antibody
    • Sialic acid binding immunoglobulin like lectin 1 antibody
    • Sialic acid-binding Ig-like lectin 1 antibody
    • Sialoadhesin antibody
    • Siglec 1 antibody
    • Siglec-1 antibody
    • Siglec1 antibody
    • SN antibody
    • SN_HUMAN antibody
    see all

Images

  • All lanes : Anti-Sialoadhesin antibody [HSn 7D2] (ab18619) at 1 µg/ml

    Lane 1 : Human spleen whole tissue lysate
    Lane 2 : Human lymph node whole tissue lysate

    Lysates/proteins at 40 µg per lane.

    Secondary
    All lanes : HRP conjugated Goat Anti-Mouse IgG (H+L) at 1/5000 dilution

    Performed under reducing conditions.

    Exposure time: 20 minutes


    This blot was produced using a 3-8% Tris-Acetate gel under the TA buffer system. The gel was run at 100V for 60 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab18619 overnight at 4°C. Antibody binding was detected using a Goat anti-mouse antibody conjugated to HRP, and visualised using ECL development solution ab133406.

  • IHC image of Sialoadhesin staining in human liver frozen tissue section, fixed in 10% paraformaldehyde (10 min). Staining was performed on a Leica Bond system using the standard protocol F. The section was then incubated with ab18619, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

References

This product has been referenced in:
  • Murakami T  et al. Secondary lymphoid organ fibroblastic reticular cells mediate trans-infection of HIV-1 via CD44-hyaluronan interactions. Nat Commun 9:2436 (2018). Read more (PubMed: 29934525) »
  • Oliveira JJ  et al. The plasma biomarker soluble SIGLEC-1 is associated with the type I interferon transcriptional signature, ethnic background and renal disease in systemic lupus erythematosus. Arthritis Res Ther 20:152 (2018). Read more (PubMed: 30053827) »
See all 7 Publications for this product

Customer reviews and Q&As

1-4 of 4 Abreviews or Q&A

Question
Answer

One reference listed in the 'Specific references' section does relate use of ab18619 in IHC. This is the Hartnell et al paper. They do not describe the use of antigen retrieval.

I would suggest trying no antigen retrieval along with a citrate buffer pH6 antigen retrieval and compare the results to see which gives the best staining.

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (Monocytes and Macrophages)
Loading amount
100000 cells
Specification
Monocytes and Macrophages
Treatment
Untreated monocytes or monocytes treated for 5 days with PMA plus 1 day PMA and hydrocortisone to differentiate them to macrophages
Gel Running Conditions
Reduced Denaturing (Pierce Precise 4 - 20 % gradient protein gels)
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C

Abcam user community

Verified customer

Submitted Sep 23 2009

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Flow Cytometry
Sample
Human Cell (Peripheral blood mononuclear cells)
Specification
Peripheral blood mononuclear cells
Preparation
Cell harvesting/tissue preparation method: Cells harvested from whole blood and PBMC isolated through a Ficoll gradient
Sample buffer: FACS buffer (PBS 0.5% FCS 0.5M EDTA)
Fixation
Paraformaldehyde
Permeabilization
No
Gating Strategy
Lympocyte and Monocyte populations

Abcam user community

Verified customer

Submitted Sep 02 2008

Answer

I can confirm that ab18619 is supplied in PBS buffer only (sterile and filtered). Please let me know if you need more information about this product,

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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