Product nameAnti-Sialoadhesin antibody [HSn 7D2]
See all Sialoadhesin primary antibodies
DescriptionMouse monoclonal [HSn 7D2] to Sialoadhesin
Tested applicationsSuitable for: ICC/IF, WB, RIA, Flow Cyt, ELISA, IHC-P, IHC-Frmore details
Species reactivityReacts with: Human
Full length native protein (purified) corresponding to Human Sialoadhesin. Full length hSn(d1-4)Fc protein.
EpitopeWithin domains 1-4.
- WB: Human spleen and human lymph node whole tissue lysate. IHC-Fr: Immunohistochemistry (Frozen sections) d) Frozen Human liver normal tissue.
This antibody clone is manufactured by Abcam. If you require a different buffer formulation or a particular conjugate for your experiments, please contact firstname.lastname@example.org.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.02% Sodium azide
Concentration information loading...
PurityProtein G purified
Clone numberHSn 7D2
Light chain typekappa
Our Abpromise guarantee covers the use of ab18619 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use at an assay dependent concentration. PubMed: 20696778|
|WB||Use a concentration of 1 µg/ml.|
|RIA||Use at an assay dependent concentration.|
|Flow Cyt||Use at an assay dependent concentration.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
|ELISA||Use at an assay dependent concentration.|
|IHC-P||Use at an assay dependent concentration.|
|IHC-Fr||Use a concentration of 5 µg/ml.|
FunctionActs as an endocytic receptor mediating clathrin dependent endocytosis. Macrophage-restricted adhesion molecule that mediates sialic-acid dependent binding to lymphocytes, including granulocytes, monocytes, natural killer cells, B-cells and CD8 T-cells. Preferentially binds to alpha-2,3-linked sialic acid (By similarity). Binds to SPN/CD43 on T-cells (By similarity). May play a role in hemopoiesis.
Tissue specificityExpressed by macrophages in various tissues. High levels are found in spleen, lymph node, perivascular macrophages in brain and lower levels in bone marrow, liver Kupffer cells and lamina propria of colon and lung. Also expressed by inflammatory macrophages in rheumatoid arthritis.
Sequence similaritiesBelongs to the immunoglobulin superfamily. SIGLEC (sialic acid binding Ig-like lectin) family.
Contains 16 Ig-like C2-type (immunoglobulin-like) domains.
Contains 1 Ig-like V-type (immunoglobulin-like) domain.
Cellular localizationSecreted and Cell membrane.
- Information by UniProt
- CD169 antibody
- CD169 antigen antibody
- dJ1009E24.1 antibody
All lanes : Anti-Sialoadhesin antibody [HSn 7D2] (ab18619) at 1 µg/ml
Lane 1 : Human spleen whole tissue lysate
Lane 2 : Human lymph node whole tissue lysate
Lysates/proteins at 40 µg per lane.
All lanes : HRP conjugated Goat Anti-Mouse IgG (H+L) at 1/5000 dilution
Performed under reducing conditions.
Exposure time: 20 minutes
This blot was produced using a 3-8% Tris-Acetate gel under the TA buffer system. The gel was run at 100V for 60 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab18619 overnight at 4°C. Antibody binding was detected using a Goat anti-mouse antibody conjugated to HRP, and visualised using ECL development solution ab133406.
IHC image of Sialoadhesin staining in human liver frozen tissue section, fixed in 10% paraformaldehyde (10 min). Staining was performed on a Leica Bond system using the standard protocol F. The section was then incubated with ab18619, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
This product has been referenced in:
- Murakami T et al. Secondary lymphoid organ fibroblastic reticular cells mediate trans-infection of HIV-1 via CD44-hyaluronan interactions. Nat Commun 9:2436 (2018). Read more (PubMed: 29934525) »
- Oliveira JJ et al. The plasma biomarker soluble SIGLEC-1 is associated with the type I interferon transcriptional signature, ethnic background and renal disease in systemic lupus erythematosus. Arthritis Res Ther 20:152 (2018). Read more (PubMed: 30053827) »