Anti-Sialoadhesin/CD169 antibody [3D6.112] (ab53443)

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Immunohistochemistry (Frozen sections) - Anti-Sialoadhesin/CD169 antibody [3D6.112] (ab53443)

    Key features and details

    • Rat monoclonal [3D6.112] to Sialoadhesin/CD169
    • Suitable for: IHC-Fr
    • Reacts with: Mouse
    • Isotype: IgG2a

    Overview

    • Product name

      Anti-Sialoadhesin/CD169 antibody [3D6.112]
      See all Sialoadhesin/CD169 primary antibodies

    • Description

      Rat monoclonal [3D6.112] to Sialoadhesin/CD169

    • Host species

      Rat

    • Tested Applications & Species

      Application Species
      IHC-Fr
      Mouse
      See all applications and species data

    • Immunogen

      Full length native protein (purified) corresponding to Mouse Sialoadhesin/CD169.

    • General notes


      Fusion partners: Spleen cells from an immunised AO rat were fused with the cells of the Y3 rat myeloma cell line.

       This product was previously labelled as Sialoadhesin

       

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

      One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

      Learn more here.

    Properties

    • Form

      Liquid

    • Storage instructions

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.

    • Storage buffer

      Preservative: 0.09% Sodium azide
      Constituent: PBS
    • Concentration information loading...

    • Purity

      Protein G purified

    • Purification notes

      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant.

    • Primary antibody notes

      Fusion partners: Spleen cells from an immunised AO rat were fused with the cells of the Y3 rat myeloma cell line.

    • Clonality

      Monoclonal

    • Clone number

      3D6.112

    • Isotype

      IgG2a

    • Research areas

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab53443 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Guaranteed

    Tested applications are guaranteed to work and covered by our Abpromise guarantee.

    Predicted

    Predicted to work for this combination of applications and species but not guaranteed.

    Incompatible

    Does not work for this combination of applications and species.

    Application Species
    IHC-Fr
    Mouse
    Application Abreviews Notes
    IHC-Fr
    1/50 - 1/100.
    Notes
    IHC-Fr
    1/50 - 1/100.

    Target

    • Function

      Acts as an endocytic receptor mediating clathrin dependent endocytosis. Macrophage-restricted adhesion molecule that mediates sialic-acid dependent binding to lymphocytes, including granulocytes, monocytes, natural killer cells, B-cells and CD8 T-cells. Preferentially binds to alpha-2,3-linked sialic acid (By similarity). Binds to SPN/CD43 on T-cells (By similarity). May play a role in hemopoiesis.

    • Tissue specificity

      Expressed by macrophages in various tissues. High levels are found in spleen, lymph node, perivascular macrophages in brain and lower levels in bone marrow, liver Kupffer cells and lamina propria of colon and lung. Also expressed by inflammatory macrophages in rheumatoid arthritis.

    • Sequence similarities

      Belongs to the immunoglobulin superfamily. SIGLEC (sialic acid binding Ig-like lectin) family.
      Contains 16 Ig-like C2-type (immunoglobulin-like) domains.
      Contains 1 Ig-like V-type (immunoglobulin-like) domain.

    • Cellular localization

      Secreted and Cell membrane.
    • Information by UniProt

    • Database links

      • Alternative names

        • CD169 antibody
        • CD169 antigen antibody
        • dJ1009E24.1 antibody
        • DKFZp667F058 antibody
        • FLJ00051 antibody
        • FLJ00055 antibody
        • FLJ00073 antibody
        • FLJ00411 antibody
        • FLJ32150 antibody
        • Sialic acid binding Ig like lectin 1 antibody
        • sialic acid binding Ig-like lectin 1, sialoadhesin antibody
        • Sialic acid binding immunoglobulin like lectin 1 antibody
        • Sialic acid-binding Ig-like lectin 1 antibody
        • Sialoadhesin antibody
        • Siglec 1 antibody
        • Siglec-1 antibody
        • Siglec1 antibody
        • SN antibody
        • SN_HUMAN antibody
        see all

      Images

      • Immunohistochemistry (Frozen sections) - Anti-Sialoadhesin/CD169 antibody [3D6.112] (ab53443)
        Immunohistochemistry (Frozen sections) - Anti-Sialoadhesin/CD169 antibody [3D6.112] (ab53443)

        ab53443 staining Sialoadhesin/CD169 in mouse spleen by Immunohistochemistry (Frozen sections).

      References (4)

      Publishing research using ab53443? Please let us know so that we can cite the reference in this datasheet.

      ab53443 has been referenced in 4 publications.

      • Zheng J  et al. Evaluation of metastatic niches in distant organs after surgical removal of tumor-bearing lymph nodes. BMC Cancer 18:608 (2018). PubMed: 29848296
      • Ibrahim MK  et al. Deficiency of lymph node-resident dendritic cells (DCs) and dysregulation of DC chemoattractants in a malnourished mouse model of Leishmania donovani infection. Infect Immun 82:3098-112 (2014). PubMed: 24818662
      • Ibrahim MK  et al. The malnutrition-related increase in early visceralization of Leishmania donovani is associated with a reduced number of lymph node phagocytes and altered conduit system flow. PLoS Negl Trop Dis 7:e2329 (2013). Mouse . PubMed: 23967356
      • Xiong YS  et al. Contribution of monocytes Siglec-1 in stimulating T cells proliferation and activation in atherosclerosis. Atherosclerosis 224:58-65 (2012). PubMed: 22789514

      Customer reviews and Q&As

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      1-2 of 2 Abreviews or Q&A

      Question

      I was told that Abcam had CD169-biotin but I cant seem to find this on the list. I would like to use it for staining mouse spleen sections (immunohistochemistry). Do you have this product?

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      Answer

      Thank you for contacting us.

      Unfortunately, we only have the non-conjugated antibody or with a FITC conjugate.

      However, you could conjugate the antibody yourself with one of our biotin conjugation kits. Please see the following datasheet links:

      https://www.abcam.com/SialoadhesinCD169-antibody-3D6112-ab53443.html (or use the following: https://www.abcam.com/SialoadhesinCD169-antibody-3D6112-ab53443.html).

      https://www.abcam.com/Biotin-type-A-Conjugation-Kit-ab102865.html (or use the following: https://www.abcam.com/Biotin-type-A-Conjugation-Kit-ab102865.html).



      I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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      Question

      Dear Sir or Madam, can you please tell me if antibody ab53443 is directed against the N-terminus of the the target protein and if I can thus use it for the detection of only the N-terminal domain? --

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      Answer

      Thank you for your inquiry. The immunogen for this antibody was the full length protein from mouse. The epitope has not been mapped by us, or anyone else to our knowledge. Therefore I am unable to answer your question. We do not know where this antibody binds to within the protein. I am sorry I did not have a positive answer for you on this occasion and hope this information is nevertheless helpful.

      Read More

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