Key features and details
- Rat monoclonal [3D6.112] to Sialoadhesin/CD169
- Suitable for: IHC-Fr
- Reacts with: Mouse
- Isotype: IgG2a
Product nameAnti-Sialoadhesin/CD169 antibody [3D6.112]
See all Sialoadhesin/CD169 primary antibodies
DescriptionRat monoclonal [3D6.112] to Sialoadhesin/CD169
Tested Applications & Species
Application Species IHC-FrMouse
Full length native protein (purified) corresponding to Mouse Sialoadhesin/CD169.
Fusion partners: Spleen cells from an immunised AO rat were fused with the cells of the Y3 rat myeloma cell line.
This product was previously labelled as Sialoadhesin
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
Storage bufferPreservative: 0.09% Sodium azide
Concentration information loading...
PurityProtein G purified
Purification notesPurified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant.
Primary antibody notesFusion partners: Spleen cells from an immunised AO rat were fused with the cells of the Y3 rat myeloma cell line.
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab53443 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
1/50 - 1/100.
1/50 - 1/100.
FunctionActs as an endocytic receptor mediating clathrin dependent endocytosis. Macrophage-restricted adhesion molecule that mediates sialic-acid dependent binding to lymphocytes, including granulocytes, monocytes, natural killer cells, B-cells and CD8 T-cells. Preferentially binds to alpha-2,3-linked sialic acid (By similarity). Binds to SPN/CD43 on T-cells (By similarity). May play a role in hemopoiesis.
Tissue specificityExpressed by macrophages in various tissues. High levels are found in spleen, lymph node, perivascular macrophages in brain and lower levels in bone marrow, liver Kupffer cells and lamina propria of colon and lung. Also expressed by inflammatory macrophages in rheumatoid arthritis.
Sequence similaritiesBelongs to the immunoglobulin superfamily. SIGLEC (sialic acid binding Ig-like lectin) family.
Contains 16 Ig-like C2-type (immunoglobulin-like) domains.
Contains 1 Ig-like V-type (immunoglobulin-like) domain.
Cellular localizationSecreted and Cell membrane.
- Information by UniProt
- CD169 antibody
- CD169 antigen antibody
- dJ1009E24.1 antibody
ab53443 has been referenced in 4 publications.
- Zheng J et al. Evaluation of metastatic niches in distant organs after surgical removal of tumor-bearing lymph nodes. BMC Cancer 18:608 (2018). PubMed: 29848296
- Ibrahim MK et al. Deficiency of lymph node-resident dendritic cells (DCs) and dysregulation of DC chemoattractants in a malnourished mouse model of Leishmania donovani infection. Infect Immun 82:3098-112 (2014). PubMed: 24818662
- Ibrahim MK et al. The malnutrition-related increase in early visceralization of Leishmania donovani is associated with a reduced number of lymph node phagocytes and altered conduit system flow. PLoS Negl Trop Dis 7:e2329 (2013). Mouse . PubMed: 23967356
- Xiong YS et al. Contribution of monocytes Siglec-1 in stimulating T cells proliferation and activation in atherosclerosis. Atherosclerosis 224:58-65 (2012). PubMed: 22789514