Anti-Sialoadhesin/CD169 antibody [3D6.112] (ab53443)

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Overview

  • Product name

    Anti-Sialoadhesin/CD169 antibody [3D6.112]
    See all Sialoadhesin/CD169 primary antibodies

  • Description

    Rat monoclonal [3D6.112] to Sialoadhesin/CD169

  • Host species

    Rat

  • Tested applications

    Suitable for: IHC-Fr, Flow Cytmore details

  • Species reactivity

    Reacts with: Mouse

  • Immunogen

    Full length native protein (purified) corresponding to Mouse Sialoadhesin/CD169.

  • General notes


    Fusion partners: Spleen cells from an immunised AO rat were fused with the cells of the Y3 rat myeloma cell line.

     This product was previously labelled as Sialoadhesin

     

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.

  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituent: PBS
  • Concentration information loading...

  • Purity

    Protein G purified

  • Purification notes

    Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant.

  • Primary antibody notes

    Fusion partners: Spleen cells from an immunised AO rat were fused with the cells of the Y3 rat myeloma cell line.

  • Clonality

    Monoclonal

  • Clone number

    3D6.112

  • Isotype

    IgG2a

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab53443 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr 1/50 - 1/100.
Flow Cyt 1/100 - 1/1000.

ab18450 - Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

 

Target

  • Function

    Acts as an endocytic receptor mediating clathrin dependent endocytosis. Macrophage-restricted adhesion molecule that mediates sialic-acid dependent binding to lymphocytes, including granulocytes, monocytes, natural killer cells, B-cells and CD8 T-cells. Preferentially binds to alpha-2,3-linked sialic acid (By similarity). Binds to SPN/CD43 on T-cells (By similarity). May play a role in hemopoiesis.

  • Tissue specificity

    Expressed by macrophages in various tissues. High levels are found in spleen, lymph node, perivascular macrophages in brain and lower levels in bone marrow, liver Kupffer cells and lamina propria of colon and lung. Also expressed by inflammatory macrophages in rheumatoid arthritis.

  • Sequence similarities

    Belongs to the immunoglobulin superfamily. SIGLEC (sialic acid binding Ig-like lectin) family.
    Contains 16 Ig-like C2-type (immunoglobulin-like) domains.
    Contains 1 Ig-like V-type (immunoglobulin-like) domain.

  • Cellular localization

    Secreted and Cell membrane.
  • Information by UniProt

  • Database links

    • Alternative names

      • CD169 antibody
      • CD169 antigen antibody
      • dJ1009E24.1 antibody
      • DKFZp667F058 antibody
      • FLJ00051 antibody
      • FLJ00055 antibody
      • FLJ00073 antibody
      • FLJ00411 antibody
      • FLJ32150 antibody
      • Sialic acid binding Ig like lectin 1 antibody
      • sialic acid binding Ig-like lectin 1, sialoadhesin antibody
      • Sialic acid binding immunoglobulin like lectin 1 antibody
      • Sialic acid-binding Ig-like lectin 1 antibody
      • Sialoadhesin antibody
      • Siglec 1 antibody
      • Siglec-1 antibody
      • Siglec1 antibody
      • SN antibody
      • SN_HUMAN antibody
      see all

    Images

    • Immunohistochemistry (Frozen sections) - Anti-Sialoadhesin/CD169 antibody [3D6.112] (ab53443)
      Immunohistochemistry (Frozen sections) - Anti-Sialoadhesin/CD169 antibody [3D6.112] (ab53443)

      ab53443 staining Sialoadhesin/CD169 in mouse spleen by Immunohistochemistry (Frozen sections).

    References

    This product has been referenced in:

    • Zheng J  et al. Evaluation of metastatic niches in distant organs after surgical removal of tumor-bearing lymph nodes. BMC Cancer 18:608 (2018). Read more (PubMed: 29848296) »
    • Ibrahim MK  et al. Deficiency of lymph node-resident dendritic cells (DCs) and dysregulation of DC chemoattractants in a malnourished mouse model of Leishmania donovani infection. Infect Immun 82:3098-112 (2014). Read more (PubMed: 24818662) »
    See all 3 Publications for this product

    Publishing research using ab53443? Please let us know so that we can cite the reference in this datasheet.

    Customer reviews and Q&As

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    1-2 of 2 Abreviews or Q&A

    Question

    I was told that Abcam had CD169-biotin but I cant seem to find this on the list. I would like to use it for staining mouse spleen sections (immunohistochemistry). Do you have this product?

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    Answer

    Thank you for contacting us.

    Unfortunately, we only have the non-conjugated antibody or with a FITC conjugate.

    However, you could conjugate the antibody yourself with one of our biotin conjugation kits. Please see the following datasheet links:

    https://www.abcam.com/index.html?datasheet=53443 (or use the following: https://www.abcam.com/index.html?datasheet=53443).

    https://www.abcam.com/index.html?datasheet=102865 (or use the following: https://www.abcam.com/index.html?datasheet=102865).



    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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    Question

    Dear Sir or Madam, can you please tell me if antibody ab53443 is directed against the N-terminus of the the target protein and if I can thus use it for the detection of only the N-terminal domain? --

    Read More
    Answer

    Thank you for your inquiry. The immunogen for this antibody was the full length protein from mouse. The epitope has not been mapped by us, or anyone else to our knowledge. Therefore I am unable to answer your question. We do not know where this antibody binds to within the protein. I am sorry I did not have a positive answer for you on this occasion and hope this information is nevertheless helpful.

    Read More

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