Key features and details
- Mouse monoclonal [HSn 7D2] to Sialoadhesin/CD169
- Suitable for: WB, IHC-Fr
- Reacts with: Human
- Isotype: IgG1
Product nameAnti-Sialoadhesin/CD169 antibody [HSn 7D2]
See all Sialoadhesin/CD169 primary antibodies
DescriptionMouse monoclonal [HSn 7D2] to Sialoadhesin/CD169
Tested applicationsSuitable for: WB, IHC-Frmore details
Species reactivityReacts with: Human
Full length native protein (purified) corresponding to Human Sialoadhesin/CD169.
EpitopeWithin domains 1-4.
- WB: Human spleen and human lymph node whole tissue lysate. IHC-Fr: Human liver normal tissue.
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact firstname.lastname@example.org.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.02% Sodium azide
Concentration information loading...
PurityProtein G purified
Clone numberHSn 7D2
Light chain typekappa
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab18619 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml.
Use a concentration of 5 µg/ml.
Use a concentration of 1 µg/ml.
Use a concentration of 5 µg/ml.
FunctionActs as an endocytic receptor mediating clathrin dependent endocytosis. Macrophage-restricted adhesion molecule that mediates sialic-acid dependent binding to lymphocytes, including granulocytes, monocytes, natural killer cells, B-cells and CD8 T-cells. Preferentially binds to alpha-2,3-linked sialic acid (By similarity). Binds to SPN/CD43 on T-cells (By similarity). May play a role in hemopoiesis.
Tissue specificityExpressed by macrophages in various tissues. High levels are found in spleen, lymph node, perivascular macrophages in brain and lower levels in bone marrow, liver Kupffer cells and lamina propria of colon and lung. Also expressed by inflammatory macrophages in rheumatoid arthritis.
Sequence similaritiesBelongs to the immunoglobulin superfamily. SIGLEC (sialic acid binding Ig-like lectin) family.
Contains 16 Ig-like C2-type (immunoglobulin-like) domains.
Contains 1 Ig-like V-type (immunoglobulin-like) domain.
Cellular localizationSecreted and Cell membrane.
- Information by UniProt
- CD169 antibody
- CD169 antigen antibody
- dJ1009E24.1 antibody
IHC image of Sialoadhesin/CD169 staining in human liver frozen tissue section, fixed in 10% paraformaldehyde (10 min). Staining was performed on a Leica Bond system using the standard protocol F. The section was then incubated with ab18619, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
All lanes : Anti-Sialoadhesin/CD169 antibody [HSn 7D2] - BSA and Azide free (ab269566) at 1 µg/ml
Lane 1 : Human spleen whole tissue lysate
Lane 2 : Human lymph node whole tissue lysate
Lysates/proteins at 30 µg per lane.
All lanes : HRP conjugated Goat Anti-Mouse IgG (H+L) at 1/5000 dilution
Performed under reducing conditions.
Exposure time: 20 minutes
This blot was produced using a 3-8% Tris-Acetate gel under the TA buffer system. The gel was run at 150V for 60 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes.
The membrane was then blocked for an hour using 2% BSA before being incubated with ab269566 overnight at 4°C. Antibody binding was detected using a Goat anti-mouse antibody conjugated to HRP, and visualised using ECL development solution ab133406.
This image was produced using ab269566, the same antibody clone but in a different PBS only formulation
ab18619 has been referenced in 8 publications.
- Li S et al. Siglec1 enhances inflammation through miR-1260-dependent degradation of I?Ba in COPD. Exp Mol Pathol 113:104398 (2020). PubMed: 32007531
- Murakami T et al. Secondary lymphoid organ fibroblastic reticular cells mediate trans-infection of HIV-1 via CD44-hyaluronan interactions. Nat Commun 9:2436 (2018). PubMed: 29934525
- Oliveira JJ et al. The plasma biomarker soluble SIGLEC-1 is associated with the type I interferon transcriptional signature, ethnic background and renal disease in systemic lupus erythematosus. Arthritis Res Ther 20:152 (2018). PubMed: 30053827
- Jans J et al. Siglec-1 inhibits RSV-induced interferon gamma production by adult T cells in contrast to newborn T cells. Eur J Immunol 48:621-631 (2018). PubMed: 29266251
- Cutler AJ et al. Capturing the systemic immune signature of a norovirus infection: an n-of-1 case study within a clinical trial. Wellcome Open Res 2:28 (2017). PubMed: 28815218
- Fujita E et al. Statin attenuates experimental anti-glomerular basement membrane glomerulonephritis together with the augmentation of alternatively activated macrophages. Am J Pathol 177:1143-54 (2010). ICC/IF ; Human . PubMed: 20696778
- Hartnell A et al. Characterization of human sialoadhesin, a sialic acid binding receptor expressed by resident and inflammatory macrophage populations. Blood 97:288-96 (2001). PubMed: 11133773
- Steiniger B et al. The species-specific structure of microanatomical compartments in the human spleen: strongly sialoadhesin-positive macrophages occur in the perifollicular zone, but not in the marginal zone. Immunology 92:307-16 (1997). PubMed: 9415041