Recombinant
RabMAb

Recombinant Anti-Sialoadhesin/CD169 antibody [SP213] - C-terminal (ab227672)

Overview

  • Product name

    Anti-Sialoadhesin/CD169 antibody [SP213] - C-terminal
    See all Sialoadhesin/CD169 primary antibodies
  • Description

    Rabbit monoclonal [SP213] to Sialoadhesin/CD169 - C-terminal
  • Host species

    Rabbit
  • Tested applications

    Suitable for: Flow Cyt, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human Sialoadhesin/CD169 aa 1650 to the C-terminus. The exact sequence is proprietary. Near the C-terminal.
    Database link: Q9BZZ2

  • Positive control

    • IHC-P: Human liver, bone marrow, colon, lung, and adrenal gland tissue; FC: Human PBMC treated with 20ng/ml IFN-gamma for 24 hours cells.
  • General notes

     This product was previously labelled as Sialoadhesin

     

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.6
    Preservative: 0.1% Sodium azide
    Constituents: PBS, 1% BSA
  • Concentration information loading...
  • Purity

    Protein A/G purified
  • Purification notes

    Purified from TCS by protein A/G.
  • Clonality

    Monoclonal
  • Clone number

    SP213
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab227672 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/20.
IHC-P 1/100.

Antigen retrieval: Boil tissue section in 10mM citrate buffer, pH 6.0 for 10 minutes followed by cooling at room temperature for 20 minutes.

Target

  • Function

    Acts as an endocytic receptor mediating clathrin dependent endocytosis. Macrophage-restricted adhesion molecule that mediates sialic-acid dependent binding to lymphocytes, including granulocytes, monocytes, natural killer cells, B-cells and CD8 T-cells. Preferentially binds to alpha-2,3-linked sialic acid (By similarity). Binds to SPN/CD43 on T-cells (By similarity). May play a role in hemopoiesis.
  • Tissue specificity

    Expressed by macrophages in various tissues. High levels are found in spleen, lymph node, perivascular macrophages in brain and lower levels in bone marrow, liver Kupffer cells and lamina propria of colon and lung. Also expressed by inflammatory macrophages in rheumatoid arthritis.
  • Sequence similarities

    Belongs to the immunoglobulin superfamily. SIGLEC (sialic acid binding Ig-like lectin) family.
    Contains 16 Ig-like C2-type (immunoglobulin-like) domains.
    Contains 1 Ig-like V-type (immunoglobulin-like) domain.
  • Cellular localization

    Secreted and Cell membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • CD169 antibody
    • CD169 antigen antibody
    • dJ1009E24.1 antibody
    • DKFZp667F058 antibody
    • FLJ00051 antibody
    • FLJ00055 antibody
    • FLJ00073 antibody
    • FLJ00411 antibody
    • FLJ32150 antibody
    • Sialic acid binding Ig like lectin 1 antibody
    • sialic acid binding Ig-like lectin 1, sialoadhesin antibody
    • Sialic acid binding immunoglobulin like lectin 1 antibody
    • Sialic acid-binding Ig-like lectin 1 antibody
    • Sialoadhesin antibody
    • Siglec 1 antibody
    • Siglec-1 antibody
    • Siglec1 antibody
    • SN antibody
    • SN_HUMAN antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human colon tissue sections labeling Sialoadhesin/CD169 with ab227672 at 1/100 dilution (1.17 µg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0, epitope retrieval solution 1) for 10 mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human liver tissue sections labeling Sialoadhesin/CD169 with ab227672 at 1/100 dilution (1.17 µg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0, epitope retrieval solution 1) for 10 mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
  • Flow Cytometry analysis of Human PBMC treated with 20ng/ml IFN-gamma for 24 hours cells labeling Sialoadhesin/CD169 with purified ab227672 at 1/20 dilution (6 µg/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 0.1% Tween-20. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150081) secondary antibody was used at 1/2000 dilution. Isotype control - Rabbit monoclonal IgG (ab172730). Unlabeled control - Unlabelled cells.

  • Formalin-fixed, paraffin-embedded human liver tissue stained for Sialoadhesin/CD169 with ab227672 at 1/100 dilution in immunohistochemical analysis.

  • Formalin-fixed, paraffin-embedded human bone marrow tissue stained for Sialoadhesin/CD169 with ab227672 at 1/100 dilution in immunohistochemical analysis.

  • Formalin-fixed, paraffin-embedded human colon tissue stained for Sialoadhesin/CD169 with ab227672 at 1/100 dilution in immunohistochemical analysis.

  • Formalin-fixed, paraffin-embedded human lung tissue stained for Sialoadhesin/CD169 with ab227672 at 1/100 dilution in immunohistochemical analysis.

  • Formalin-fixed, paraffin-embedded human adrenal gland tissue stained for Sialoadhesin/CD169 with ab227672 at 1/100 dilution in immunohistochemical analysis.

References

ab227672 has not yet been referenced specifically in any publications.

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