Recombinant
RabMAb

Recombinant Anti-Sialoadhesin/CD169 antibody [SP216] - BSA and Azide free (ab245742)

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Overview

  • Product name

    Anti-Sialoadhesin/CD169 antibody [SP216] - BSA and Azide free
    See all Sialoadhesin/CD169 primary antibodies

  • Description

    Rabbit monoclonal [SP216] to Sialoadhesin/CD169 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: Flow Cyt, IHC-Pmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide within Human Sialoadhesin/CD169 aa 1650 to the C-terminus (internal sequence). The exact sequence is proprietary.
    Database link: Q9BZZ2

  • Positive control

    • IHC-P: Human bone marrow, HK lymphoma, squamous cell cervical carcinoma, squamous cell lung carcinoma, lung and lymph node tissue. FC: Human PBMC treated with 20ng/ml IFN-gamma for 24 hours cells

  • General notes

    ab245742 is the carrier-free version of ab183356 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    Ab245742 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product was previously labelled as Sialoadhesin

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab245742 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    Acts as an endocytic receptor mediating clathrin dependent endocytosis. Macrophage-restricted adhesion molecule that mediates sialic-acid dependent binding to lymphocytes, including granulocytes, monocytes, natural killer cells, B-cells and CD8 T-cells. Preferentially binds to alpha-2,3-linked sialic acid (By similarity). Binds to SPN/CD43 on T-cells (By similarity). May play a role in hemopoiesis.

  • Tissue specificity

    Expressed by macrophages in various tissues. High levels are found in spleen, lymph node, perivascular macrophages in brain and lower levels in bone marrow, liver Kupffer cells and lamina propria of colon and lung. Also expressed by inflammatory macrophages in rheumatoid arthritis.

  • Sequence similarities

    Belongs to the immunoglobulin superfamily. SIGLEC (sialic acid binding Ig-like lectin) family.
    Contains 16 Ig-like C2-type (immunoglobulin-like) domains.
    Contains 1 Ig-like V-type (immunoglobulin-like) domain.

  • Cellular localization

    Secreted and Cell membrane.
  • Information by UniProt

  • Database links

    • Alternative names

      • CD169 antibody
      • CD169 antigen antibody
      • dJ1009E24.1 antibody
      • DKFZp667F058 antibody
      • FLJ00051 antibody
      • FLJ00055 antibody
      • FLJ00073 antibody
      • FLJ00411 antibody
      • FLJ32150 antibody
      • Sialic acid binding Ig like lectin 1 antibody
      • sialic acid binding Ig-like lectin 1, sialoadhesin antibody
      • Sialic acid binding immunoglobulin like lectin 1 antibody
      • Sialic acid-binding Ig-like lectin 1 antibody
      • Sialoadhesin antibody
      • Siglec 1 antibody
      • Siglec-1 antibody
      • Siglec1 antibody
      • SN antibody
      • SN_HUMAN antibody
      see all

    Images

    • Flow Cytometry - Anti-Sialoadhesin/CD169 antibody [SP216] - BSA and Azide free (ab245742)
      Flow Cytometry - Anti-Sialoadhesin/CD169 antibody [SP216] - BSA and Azide free (ab245742)

      Flow Cytometry analysis of Human PBMC treated with 20ng/ml IFN-gamma for 24 hours cells labeling Sialoadhesin/CD169 with purified ab183356 at 1/20 dilution (47 µg/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 0.1% Tween-20. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150081) secondary antibody was used at 1/2000 dilution. Isotype control - Rabbit monoclonal IgG (ab172730) / Black. Unlabeled control - Unlabelled cells / blue.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab183356).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sialoadhesin/CD169 antibody [SP216] - BSA and Azide free (ab245742)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sialoadhesin/CD169 antibody [SP216] - BSA and Azide free (ab245742)

      Immunohistochemical analysis of formalin fixed, paraffin embedded human bone marrow tissue sections labeling Sialoadhesin/CD169 using ab183356 at a 1/100 dilution.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (ab183356).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sialoadhesin/CD169 antibody [SP216] - BSA and Azide free (ab245742)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sialoadhesin/CD169 antibody [SP216] - BSA and Azide free (ab245742)

      Immunohistochemical analysis of formalin fixed, paraffin embedded human HK lymphoma tissue sections labeling Sialoadhesin/CD169 using ab183356 at a 1/100 dilution.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (ab183356).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sialoadhesin/CD169 antibody [SP216] - BSA and Azide free (ab245742)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sialoadhesin/CD169 antibody [SP216] - BSA and Azide free (ab245742)

      Immunohistochemical analysis of formalin fixed, paraffin embedded human squamous cell cervical carcinoma tissue sections labeling Sialoadhesin/CD169 using ab183356 at a 1/100 dilution.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (ab183356).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sialoadhesin/CD169 antibody [SP216] - BSA and Azide free (ab245742)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sialoadhesin/CD169 antibody [SP216] - BSA and Azide free (ab245742)

      Immunohistochemical analysis of formalin fixed, paraffin embedded human squamous cell lung carcinoma tissue sections labeling Sialoadhesin/CD169 using ab183356 at a 1/100 dilution.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (ab183356).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sialoadhesin/CD169 antibody [SP216] - BSA and Azide free (ab245742)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sialoadhesin/CD169 antibody [SP216] - BSA and Azide free (ab245742)

      Immunohistochemical analysis of formalin fixed, paraffin embedded human lung tissue sections labeling Sialoadhesin/CD169 using ab183356 at a 1/100 dilution.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (ab183356).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sialoadhesin/CD169 antibody [SP216] - BSA and Azide free (ab245742)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sialoadhesin/CD169 antibody [SP216] - BSA and Azide free (ab245742)

      Immunohistochemical analysis of formalin fixed, paraffin embedded human lymph node tissue sections labeling Sialoadhesin/CD169 using ab183356 at a 1/100 dilution.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (ab183356).

    References

    ab245742 has not yet been referenced specifically in any publications.

    Publishing research using ab245742? Please let us know so that we can cite the reference in this datasheet.

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