RecombinantRabMAb

Recombinant Anti-Sialoadhesin/CD169 antibody [SP216] - BSA and Azide free (ab245742)

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Flow Cytometry - Anti-Sialoadhesin/CD169 antibody [SP216] - BSA and Azide free (ab245742)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sialoadhesin/CD169 antibody [SP216] - BSA and Azide free (ab245742)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sialoadhesin/CD169 antibody [SP216] - BSA and Azide free (ab245742)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sialoadhesin/CD169 antibody [SP216] - BSA and Azide free (ab245742)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sialoadhesin/CD169 antibody [SP216] - BSA and Azide free (ab245742)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sialoadhesin/CD169 antibody [SP216] - BSA and Azide free (ab245742)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sialoadhesin/CD169 antibody [SP216] - BSA and Azide free (ab245742)
  • Anti-Sialoadhesin/CD169 antibody [SP216] - BSA and Azide free (ab245742)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [SP216] to Sialoadhesin/CD169 - BSA and Azide free
  • Suitable for: Flow Cyt, IHC-P
  • Reacts with: Human

Overview

  • Product name

    Anti-Sialoadhesin/CD169 antibody [SP216] - BSA and Azide free
    See all Sialoadhesin/CD169 primary antibodies

  • Description

    Rabbit monoclonal [SP216] to Sialoadhesin/CD169 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: Flow Cyt, IHC-Pmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide within Human Sialoadhesin/CD169 aa 1650 to the C-terminus (internal sequence). The exact sequence is proprietary.
    Database link: Q9BZZ2

  • Positive control

    • IHC-P: Human bone marrow, HK lymphoma, squamous cell cervical carcinoma, squamous cell lung carcinoma, lung and lymph node tissue. Flow Cyt: Human PBMC treated with 20ng/ml IFN-gamma for 24 hours cells

  • General notes

    FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.

    ab245742 is the carrier-free version of ab183356 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    Ab245742 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product was previously labelled as Sialoadhesin

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Applications

Our Abpromise guarantee covers the use of ab245742 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    Acts as an endocytic receptor mediating clathrin dependent endocytosis. Macrophage-restricted adhesion molecule that mediates sialic-acid dependent binding to lymphocytes, including granulocytes, monocytes, natural killer cells, B-cells and CD8 T-cells. Preferentially binds to alpha-2,3-linked sialic acid (By similarity). Binds to SPN/CD43 on T-cells (By similarity). May play a role in hemopoiesis.

  • Tissue specificity

    Expressed by macrophages in various tissues. High levels are found in spleen, lymph node, perivascular macrophages in brain and lower levels in bone marrow, liver Kupffer cells and lamina propria of colon and lung. Also expressed by inflammatory macrophages in rheumatoid arthritis.

  • Sequence similarities

    Belongs to the immunoglobulin superfamily. SIGLEC (sialic acid binding Ig-like lectin) family.
    Contains 16 Ig-like C2-type (immunoglobulin-like) domains.
    Contains 1 Ig-like V-type (immunoglobulin-like) domain.

  • Cellular localization

    Secreted and Cell membrane.
  • Information by UniProt

  • Database links

    • Alternative names

      • CD169 antibody
      • CD169 antigen antibody
      • dJ1009E24.1 antibody
      • DKFZp667F058 antibody
      • FLJ00051 antibody
      • FLJ00055 antibody
      • FLJ00073 antibody
      • FLJ00411 antibody
      • FLJ32150 antibody
      • Sialic acid binding Ig like lectin 1 antibody
      • sialic acid binding Ig-like lectin 1, sialoadhesin antibody
      • Sialic acid binding immunoglobulin like lectin 1 antibody
      • Sialic acid-binding Ig-like lectin 1 antibody
      • Sialoadhesin antibody
      • Siglec 1 antibody
      • Siglec-1 antibody
      • Siglec1 antibody
      • SN antibody
      • SN_HUMAN antibody
      see all

    Images

    • Flow Cytometry - Anti-Sialoadhesin/CD169 antibody [SP216] - BSA and Azide free (ab245742)
      Flow Cytometry - Anti-Sialoadhesin/CD169 antibody [SP216] - BSA and Azide free (ab245742)

      Flow Cytometry analysis of Human PBMC treated with 20ng/ml IFN-gamma for 24 hours cells labeling Sialoadhesin/CD169 with purified ab183356 at 1/20 dilution (47 µg/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 0.1% Tween-20. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150081) secondary antibody was used at 1/2000 dilution. Isotype control - Rabbit monoclonal IgG (ab172730) / Black. Unlabeled control - Unlabelled cells / blue.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab183356).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sialoadhesin/CD169 antibody [SP216] - BSA and Azide free (ab245742)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sialoadhesin/CD169 antibody [SP216] - BSA and Azide free (ab245742)

      Immunohistochemical analysis of formalin fixed, paraffin embedded human bone marrow tissue sections labeling Sialoadhesin/CD169 using ab183356 at a 1/100 dilution.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (ab183356).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sialoadhesin/CD169 antibody [SP216] - BSA and Azide free (ab245742)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sialoadhesin/CD169 antibody [SP216] - BSA and Azide free (ab245742)

      Immunohistochemical analysis of formalin fixed, paraffin embedded human HK lymphoma tissue sections labeling Sialoadhesin/CD169 using ab183356 at a 1/100 dilution.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (ab183356).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sialoadhesin/CD169 antibody [SP216] - BSA and Azide free (ab245742)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sialoadhesin/CD169 antibody [SP216] - BSA and Azide free (ab245742)

      Immunohistochemical analysis of formalin fixed, paraffin embedded human squamous cell cervical carcinoma tissue sections labeling Sialoadhesin/CD169 using ab183356 at a 1/100 dilution.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (ab183356).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sialoadhesin/CD169 antibody [SP216] - BSA and Azide free (ab245742)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sialoadhesin/CD169 antibody [SP216] - BSA and Azide free (ab245742)

      Immunohistochemical analysis of formalin fixed, paraffin embedded human squamous cell lung carcinoma tissue sections labeling Sialoadhesin/CD169 using ab183356 at a 1/100 dilution.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (ab183356).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sialoadhesin/CD169 antibody [SP216] - BSA and Azide free (ab245742)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sialoadhesin/CD169 antibody [SP216] - BSA and Azide free (ab245742)

      Immunohistochemical analysis of formalin fixed, paraffin embedded human lung tissue sections labeling Sialoadhesin/CD169 using ab183356 at a 1/100 dilution.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (ab183356).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sialoadhesin/CD169 antibody [SP216] - BSA and Azide free (ab245742)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sialoadhesin/CD169 antibody [SP216] - BSA and Azide free (ab245742)

      Immunohistochemical analysis of formalin fixed, paraffin embedded human lymph node tissue sections labeling Sialoadhesin/CD169 using ab183356 at a 1/100 dilution.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (ab183356).

    • Anti-Sialoadhesin/CD169 antibody [SP216] - BSA and Azide free (ab245742)
      Anti-Sialoadhesin/CD169 antibody [SP216] - BSA and Azide free (ab245742)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (0)

    Publishing research using ab245742? Please let us know so that we can cite the reference in this datasheet.

    ab245742 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com