Overview

  • Product name
  • Description
    Rabbit polyclonal to Siglec 7
  • Host species
    Rabbit
  • Tested applications
    Suitable for: IHC-P, ELISA, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide (10-30 amino acids in length) conjugated to KLH, from the N terminal of first 50 amino acids of Human Siglec 7.

  • Positive control
    • Mouse liver tissue lysate, HL60 cell lysate, Human breast carcinoma tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab38574 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/100.
ELISA 1/1000.
WB 1/100 - 1/500. Detects a band of approximately 51 kDa (predicted molecular weight: 51 kDa).

Target

  • Function
    Putative adhesion molecule that mediates sialic-acid dependent binding to cells. Preferentially binds to alpha-2,3- and alpha-2,6-linked sialic acid. Also binds disialogangliosides (disialogalactosyl globoside, disialyl lactotetraosylceramide and disialyl GalNAc lactotetraoslylceramide). The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface. In the immune response, may act as an inhibitory receptor upon ligand induced tyrosine phosphorylation by recruiting cytoplasmic phosphatase(s) via their SH2 domain(s) that block signal transduction through dephosphorylation of signaling molecules. Mediates inhibition of natural killer cells cytotoxicity. May play a role in hemopoiesis. Inhibits differentiation of CD34+ cell precursors towards myelomonocytic cell lineage and proliferation of leukemic myeloid cells (in vitro).
  • Tissue specificity
    Predominantly expressed by resting and activated natural killer cells and at lower levels by granulocytes and monocytes. High expression found in placenta, liver, lung, spleen, and peripheral blood leukocytes.
  • Sequence similarities
    Belongs to the immunoglobulin superfamily. SIGLEC (sialic acid binding Ig-like lectin) family.
    Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
    Contains 1 Ig-like V-type (immunoglobulin-like) domain.
  • Domain
    Contains 1 copy of a cytoplasmic motif that is referred to as the immunoreceptor tyrosine-based inhibitor motif (ITIM). This motif is involved in modulation of cellular responses. The phosphorylated ITIM motif can bind the SH2 domain of several SH2-containing phosphatases.
  • Post-translational
    modifications
    Tyrosine phosphorylated.
  • Cellular localization
    Membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Adhesion inhibitory receptor molecule 1 antibody
    • Adhesion inhibitory receptor molecule 1, siglec 7 antibody
    • AIRM 1 antibody
    • AIRM-1 antibody
    • AIRM1 antibody
    • CD328 antibody
    • CD328 antigen antibody
    • CDw328 antibody
    • D siglec antibody
    • D-siglec antibody
    • HLDA 8 antibody
    • p75 antibody
    • p75/AIRM1 antibody
    • QA79 antibody
    • QA79 membrane protein antibody
    • Sialic acid binding Ig like lectin 7 antibody
    • Sialic acid binding immunoglobulin like lectin 7 antibody
    • Sialic acid-binding Ig-like lectin 7 antibody
    • SIGL7_HUMAN antibody
    • Siglec-7 antibody
    • SIGLEC19P antibody
    • SIGLEC7 antibody
    • SIGLECP2 antibody
    see all

Images

  • ab38574 staining Siglec 7 in Human breast carcinoma by Immunohistochemistry, Formalin fixed and Paraffin embedded tissue. ab38574 was peroxidase-conjugated to the secondary antibody, followed by AEC staining.
  • Western blot analysis of anti-SIGLEC7 Pab (ab38574) from left to right in HL60, k562, NCI-H460 cell line lysate (35ug/lane).

References

ab38574 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Answer

Vielen Dank für Ihre Antwort und für diese weiteren Informationen. Herzlichen Dank auch, dass Sie und andere Kollegen wirklich alles versucht haben, um mit diesen Siglec-Antikörpern positive Resultate zu erzielen. Ich weiß, dass Sie sehr viel Zeit, Energie und Ressourcen in Ihre Experimente investiert haben, und darum tut es mir umso mehr leid, dass Sie bisher noch kein positives Ergebnis haben! Da Sie offenbar gar kein Signal bekommen - insbesondere nicht auf den Positivkontrollen - würde ich Ihnen gerne den kostenlosen Ersatz für ab38574 und ab59942 anbieten. Dies könnte jeweils ein neues Vial desselben Antikörpers sein, oder alternative Siglec-7 bzw. -10-Antikörper wie beispielsweise ab38573 (bei der Auswahl bin ich gerne behilflich) oder jeder andere primäre Antikörper Ihrer Wahl. Falls Sie von all dem die Nase voll haben, stelle ich Ihnen natürlich auch gerne eine Gutschrift/Rückerstattung aus. Vielen Dank nochmal für Ihre Kooperation und Hilfsbereitschaft. Ich freue mich, wieder von Ihnen zu hören.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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