Overview

  • Product name
    Anti-SIGLEC15 antibody - C-terminal
    See all SIGLEC15 primary antibodies
  • Description
    Rabbit Polyclonal to SIGLEC15 - C-terminal
  • Host species
    Rabbit
  • Tested applications
    Suitable for: IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human SIGLEC15 aa 281-310 (C terminal) conjugated to Keyhole Limpet Haemocyanin (KLH). The exact sequence is proprietary.
    Database link: Q6ZMC9

  • Positive control
    • Human lung tissue; K562 cell line lysate.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.09% Sodium azide
    Constituent: 99% BSA
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    ab170314 is purified through a protein A column, followed by peptide affinity purification.
  • Clonality
    Polyclonal
  • Isotype
    IgG

Applications

Our Abpromise guarantee covers the use of ab170314 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/10 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/100 - 1/500. Predicted molecular weight: 36 kDa.

Target

  • Function
    Binds sialylated glycoproteins.
  • Tissue specificity
    Expressed in macrophage and/or dendritic cells of spleen and lymph nodes.
  • Sequence similarities
    Belongs to the immunoglobulin superfamily. SIGLEC (sialic acid binding Ig-like lectin) family.
    Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
    Contains 1 Ig-like V-type (immunoglobulin-like) domain.
  • Cellular localization
    Membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • CD33 antigen-like 3 antibody
    • CD33 molecule-like 3 antibody
    • CD33L3 antibody
    • HsT1361 antibody
    • Sialic acid-binding Ig-like lectin 15 antibody
    • SIG15_HUMAN antibody
    • Siglec-15 antibody
    • SIGLEC15 antibody
    see all

Images

  • Anti-SIGLEC15 antibody - C-terminal (ab170314) at 1/100 dilution + K562 cell line lysate at 35 µg

    Predicted band size: 36 kDa

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human lung tissue labeling SIGLEC15 with ab170314 at 1/10 dilution, followed by a peroxidase conjugated secondary antibody and DAB staining.

References

This product has been referenced in:

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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