Key features and details
- Mouse monoclonal [1A5] to SIGLEC5
- Suitable for: IHC-Fr, ELISA, Flow Cyt, ICC/IF, IP, Neutralising, WB
- Reacts with: Human
- Isotype: IgG1
Product nameAnti-SIGLEC5 antibody [1A5]
See all SIGLEC5 primary antibodies
DescriptionMouse monoclonal [1A5] to SIGLEC5
SpecificityThis antibody cross reacts with SEGLEC14.
Tested applicationsSuitable for: IHC-Fr, ELISA, Flow Cyt, ICC/IF, IP, Neutralising, WBmore details
Species reactivityReacts with: Human
Siglec-5-Fc protein, containing entire extracellular region of siglec-5 fused with the Fc region of human IgG1.
- Siglec 5 transfected CHO cells.
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Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Storage bufferConstituent: PBS
Concentration information loading...
PurityProtein A/G purified
Our Abpromise guarantee covers the use of ab97426 in the following tested applications.
|IHC-Fr||Use at an assay dependent concentration.|
|ELISA||Use at an assay dependent concentration.|
|Flow Cyt||Use 1µg for 106 cells.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
|ICC/IF||Use at an assay dependent concentration.|
|IP||Use at an assay dependent concentration.|
|Neutralising||Use at an assay dependent concentration.|
|WB||Use at an assay dependent concentration. Predicted molecular weight: 61 kDa.|
FunctionPutative adhesion molecule that mediates sialic-acid dependent binding to cells. Binds equally to alpha-2,3-linked and alpha-2,6-linked sialic acid. The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface.
Tissue specificityExpressed by monocytic/myeloid lineage cells. Found at high levels in peripheral blood leukocytes, spleen, bone marrow and at lower levels in lymph node, lung, appendix, placenta, pancreas and thymus. Expressed by monocytes and neutrophils but absent from leukemic cell lines representing early stages of myelomonocytic differentiation.
Sequence similaritiesBelongs to the immunoglobulin superfamily. SIGLEC (sialic acid binding Ig-like lectin) family.
Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
Contains 1 Ig-like V-type (immunoglobulin-like) domain.
DomainContains 1 copy of a cytoplasmic motif that is referred to as the immunoreceptor tyrosine-based inhibitor motif (ITIM). This motif is involved in modulation of cellular responses. The phosphorylated ITIM motif can bind the SH2 domain of several SH2-containing phosphatases.
- Information by UniProt
- CD170 antibody
- CD170 antigen antibody
- CD33 antigen-like 2 antibody
Overlay histogram showing THP1 cells stained with ab97426 (red line). The cells were fixed with 80% methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab97426, 1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.
ab97426 has been referenced in 1 publication.
- Cornish AL et al. Characterization of siglec-5, a novel glycoprotein expressed on myeloid cells related to CD33. Blood 92:2123-32 (1998). PubMed: 9731071