Key features and details
- Rabbit polyclonal to SIGLEC6
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-SIGLEC6 antibody
See all SIGLEC6 primary antibodies
DescriptionRabbit polyclonal to SIGLEC6
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Human
- IHC-P: Human placenta, liver, colon and testis tissue. WB: SIGLEC6-over expressed HEK-293T lysate.
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Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab262851 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/500 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||Use a concentration of 0.4 µg/ml.|
FunctionPutative adhesion molecule that mediates sialic-acid dependent binding to cells. Binds to alpha-2,6-linked sialic acid. The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface.
Tissue specificityExpressed at high levels in placenta (cyto- and syncytiotrophoblastic cells) and at lower levels in spleen, peripheral blood leukocytes (predominantly B-cells) and small intestine.
Sequence similaritiesBelongs to the immunoglobulin superfamily. SIGLEC (sialic acid binding Ig-like lectin) family.
Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
Contains 1 Ig-like V-type (immunoglobulin-like) domain.
DomainContains 1 copy of a cytoplasmic motif that is referred to as the immunoreceptor tyrosine-based inhibitor motif (ITIM). This motif is involved in modulation of cellular responses. The phosphorylated ITIM motif can bind the SH2 domain of several SH2-containing phosphatases.
Cellular localizationSecreted and Cell membrane.
- Information by UniProt
- CD327 antibody
- CD33 antigen-like 1 antibody
- CD33L antibody
Paraffin-embedded Human placenta tissue stained for SIGLEC6 using ab262851 at 1/500 dilution in immunohistochemical analysis.
Anti-SIGLEC6 antibody (ab262851) at 0.4 µg/ml + SIGLEC6-overexpressed HEK-293T lysate
Paraffin-embedded Human colon tissue stained for SIGLEC6 using ab262851 at 1/500 dilution in immunohistochemical analysis.
Paraffin-embedded Human testis tissue stained for SIGLEC6 using ab262851 at 1/500 dilution in immunohistochemical analysis.
Paraffin-embedded Human liver tissue stained for SIGLEC6 using ab262851 at 1/500 dilution in immunohistochemical analysis.
Paraffin-embedded Human prostate tissue stained for SIGLEC6 using ab262851 at 1/500 dilution in immunohistochemical analysis.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab262851 has not yet been referenced specifically in any publications.