Product nameAnti-SIGLEC6 antibody
See all SIGLEC6 primary antibodies
DescriptionRabbit polyclonal to SIGLEC6
Tested applicationsSuitable for: ELISA, WB, IHC-Pmore details
Species reactivityReacts with: Mouse, Human
Synthetic peptide (10-30 aa in length) at the C-term of last 100 aa of human SIGLEC6, conjugated to KLH.
- mouse spleen tissue lysate, human breast carcinoma
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Concentration information loading...
PurityProtein G purified
Our Abpromise guarantee covers the use of ab38581 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-P: Use at an assay dependent dilution (PMID 18974375).
WB: 1/100 - 1/500. Detects a band of approximately 58 kDa (predicted molecular weight: 49 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionPutative adhesion molecule that mediates sialic-acid dependent binding to cells. Binds to alpha-2,6-linked sialic acid. The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface.
Tissue specificityExpressed at high levels in placenta (cyto- and syncytiotrophoblastic cells) and at lower levels in spleen, peripheral blood leukocytes (predominantly B-cells) and small intestine.
Sequence similaritiesBelongs to the immunoglobulin superfamily. SIGLEC (sialic acid binding Ig-like lectin) family.
Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
Contains 1 Ig-like V-type (immunoglobulin-like) domain.
DomainContains 1 copy of a cytoplasmic motif that is referred to as the immunoreceptor tyrosine-based inhibitor motif (ITIM). This motif is involved in modulation of cellular responses. The phosphorylated ITIM motif can bind the SH2 domain of several SH2-containing phosphatases.
Cellular localizationSecreted and Cell membrane.
- Information by UniProt
- CD327 antibody
- CD33 antigen-like 1 antibody
- CD33L antibody
Immunohistochemical analysis of SIGLEC6 expression in formalin-fixed and paraffin-embedded human breast carcinoma tissue using 1/50 ab38581.
Anti-SIGLEC6 antibody (ab38581) at 1/100 dilution + Mouse spleen tissue lysate
Predicted band size: 49 kDa
Observed band size: 56 kDa why is the actual band size different from the predicted?
This product has been referenced in:
- Li H et al. Differential Proteomic Analysis of Syncytiotrophoblast Extracellular Vesicles from Early-Onset Severe Preeclampsia, using 8-Plex iTRAQ Labeling Coupled with 2D Nano LC-MS/MS. Cell Physiol Biochem 36:1116-30 (2015). Read more (PubMed: 26113202) »
- Chng WJ et al. Gene expression profiling of pulmonary mucosa-associated lymphoid tissue (MALT) lymphoma identifies new biological insights with potential diagnostic and therapeutic applications. Blood : (2008). IHC-P ; Human . Read more (PubMed: 18974375) »